scholarly journals Prevalence of Toxoplasma gondii indirect fluorescent antibodies in naturally- and experimentally-infected chickens (Gallus domesticus) in Thailand

2009 ◽  
Vol 54 (3) ◽  
Author(s):  
Kamlang Chumpolbanchorn ◽  
Pacharaporn Anankeatikul ◽  
Wantanee Ratanasak ◽  
Jitbanjong Wiengcharoen ◽  
R. Andrew Thompson ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Health Risks ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Gallus Domesticus ◽  
Indirect Fluorescent Antibody ◽  
Serum Samples ◽  
Public Health Risks ◽  
Potential Public Health

AbstractToxoplasma gondii infections in free-range (FR) chickens (Gallus domesticus) are potential public health risks. Antibodies for T. gondii were found in 194 out of 303 serum samples (64.03%) from FR chickens in Thailand tested by the indirect fluorescent antibody test (IFAT, 1:16). To verify the validity of serologic data in this survey, sera from chickens experimentally infected with the RH strain of T. gondii were tested by the IFAT. Antibodies against T. gondii were detected as early as 7 days p.i., peaked at 2 weeks, and then declined by 10 weeks p.i.

scholarly journals Seroprevalence of Toxoplasma gondii infection in swine matrices in Nova Mutum and Diamantino, Mato Grosso, Brazil

2010 ◽  
Vol 19 (4) ◽  
pp. 254-255 ◽  
Author(s):  
Lívia Saab Muraro ◽  
João Garcia Caramori Júnior ◽  
Maria Regina Reis Amendoeira ◽  
Joyce Alves Pereira ◽  
João Xavier de Oliveira Filho ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
The State ◽  
Indirect Fluorescent Antibody ◽  
Risk Of Infection ◽  
Serum Samples ◽  
Mato Grosso ◽  
Toxoplasma Gondii Infection

This report aimed to assess the seroprevalence of Toxoplasma gondii infection in 708 swine matrices in Nova Mutum and Diamantino in the state of Mato Grosso, Central-West Brazil. Serum samples were examined by indirect fluorescent antibody test (IFAT). It was found a seroprevalence of 12.8%, considering titers >64. Therefore, the data reinforce the need for appropriate management of swine raising to minimize the risk of infection of pigs with T. gondii.

scholarly journals Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs

2004 ◽  
Vol 24 (4) ◽  
pp. 199-202 ◽  
Author(s):  
Alessandro Pelegrine Minho ◽  
Roberta Lemos Freire ◽  
Odilon Vidotto ◽  
Solange Maria Gennari ◽  
Elizabete Marangoni Marana ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Confidence Interval ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Antibody Detection ◽  
Indirect Fluorescent Antibody ◽  
Serum Samples ◽  
Modified Agglutination Test

The study determined the sensitivity and specificity of the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT) for anti-Toxoplasma gondii antibody detection by analyzing sera from 46 experimentally infected pigs. Values for sensitivity were 95.7% (confidence interval 95%: 84.0-99.2%) and for specificity 97.8% (confidence interval 95%: 87.0-99.9%) in both tests. There was an optimum agreement of results between IFAT and MAT evidenced by a Kappa test of 0.86. These results validate these tests for the detection of T. gondii infection in pigs. IFAT and MAT despite methodologies with different characteristics and readings have similar accuracy in pig serum samples.

Investigation of Anti-Toxoplasma gondii Antibodies in Cats of the Ankara Region of Turkey Using the Sabin-Feldman Dye Test and an Indirect Fluorescent Antibody Test

2008 ◽  
Vol 94 (4) ◽  
pp. 817-820 ◽  
Author(s):  
Ayşegül Taylan Özkan ◽  
Bekir Çelebi ◽  
Cahit Babür ◽  
Araceli Lucio-Forster ◽  
Dwight D. Bowman ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Indirect Fluorescent Antibody

scholarly journals Comparison of Enzyme-Linked Immunosorbent Assay, Indirect Fluorescent Antibody Test, and Direct Agglutination Test for Detecting Toxoplasma Gondii Antibodies in Naturally Aborted Ovine Fetuses

1989 ◽  
Vol 1 (2) ◽  
pp. 124-127 ◽  
Author(s):  
Sheryl L. Seefeldt ◽  
Clyde A. Kirkbride ◽  
Jitender P. Dubey
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Agglutination Test ◽  
Indirect Fluorescent Antibody ◽  
Special Equipment

Results obtained in an enzyme-linked immunosorbent assay (ELISA), an indirect fluorescent antibody test (IFA), and a modified direct agglutination test (MAT) for Toxoplasma gondii antibodies from examination of fetal fluids from 377 aborted ovine fetuses were compared. Sixty-seven samples were positive by MAT (titers 1:16 to > 1:65,536), 58 were positive by ELISA, and 62 were positive by immunoglobulin G-IFA. The MAT was preferred because it required less time, labor, and special equipment. It was simple to run, could be done on serum from any species without modification, and it was more effective than the IFA for detecting toxoplasma antibodies in severely autolyzed fetuses. No advantage was found in determining immunoglobulin M antibodies in ovine fetal sera.

scholarly journals A Comparative Study of an Indirect Fluorescent Antibody Test and an Immunoperoxidase Monolayer Assay for the Diagnosis of Porcine Proliferative Enteropathy

2002 ◽  
Vol 14 (5) ◽  
pp. 420-423 ◽  
Author(s):  
Roberto M. C. Guedes ◽  
Connie J. Gebhart ◽  
Nathan L. Winkelman ◽  
Rebecca A. Mackie-Nuss
Keyword(s):  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Indirect Fluorescent Antibody ◽  
Lawsonia Intracellularis ◽  
Serum Samples ◽  
Proliferative Enteropathy ◽  
Formalin Fixed Paraffin ◽  
Oral Inoculation ◽  
Formalin Fixed Paraffin Embedded

The currently used indirect fluorescent antibody test (IFAT) for the detection of antibodies against porcine proliferative enteropathy (PPE) was compared to an immunoperoxidase monolayer assay (IPMA). Serum samples used in this comparison were collected from 5-week-old pigs on day 0 (pre-experimental challenge) and on days 7, 14, 21, and 28 after oral inoculation with intestinal homogenate from pigs affected by PPE (28 challenged pigs) and sucrose phosphate glutamate solution (2 control pigs). All animals were euthanized 4 weeks after inoculation. Immunohistochemistry staining was applied to formalin-fixed, paraffin-embedded sections of ileum for the detection of Lawsonia intracellularis antigen. The serology results with each method agreed in all samples, except on days 0 and 7 in 1 control animal, which was positive by IPMA, but negative by IFAT. The percentage of agreement between IFAT and IPMA was 98.6%.

Comparison of indirect fluorescent antibody test and the modified agglutination test for the detection of Toxoplasma gondii antibodies in stray dogs from Southern Brazil

2016 ◽  
Vol 61 (4) ◽  
Author(s):  
Jonatas Campos de Almeida ◽  
Michelle Salmon Frehse ◽  
Italmar Teodorico Navarro ◽  
João Luis Garcia ◽  
Alexander Welker Biondo ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Indirect Fluorescent Antibody ◽  
Southern Brazil ◽  
Stray Dogs ◽  
Modified Agglutination Test

AbstractThe aim of the present study was to determine the prevalence of antibodies to

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