Fasciola hepatica — the pilot study of in vitro assessing immune response against native and recombinant antigens of the fluke

2013 ◽  
Vol 58 (4) ◽  
Author(s):  
Piotr Bąska ◽  
Anna Zawistowska-Deniziak ◽  
Anna Zdziarska ◽  
Katarzyna Wasyl ◽  
Marcin Wiśniewski ◽  
...  
Keyword(s):  
Immune Response ◽  
Cell Line ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Effective Vaccine ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Recombinant Antigens ◽  
Tnf Α

AbstractFasciola hepatica is a liver fluke that infects 2.4 million of people and causes great economical loss in animal production. To date a 100% effective vaccine has not been developed and the disease is controlled by drug therapy. Great efforts are put into development of effective vaccine against parasite what is difficult since Fasciola spp. (like other helmints) during evolutionary process has developed sophisticated and efficient methods to evade immune response. During preliminary experiments it is convenient to use cell lines which are relatively cheap and allow for reproducible comparison of results between laboratories. We stimulated BOMA (bovine monocyte/macrophage cell line) and BOMAC (bovine macrophage cell line) with native or recombinant antigens of Fasciola hepatica and assessed IFN-γ, IL-4 and TNF-α level upon stimulation. We observed diminished secretion of proinflammatory TNF-α in LPS activated BOMA cells stimulated with Excretory/Secretory products of adult fluke (Fh-ES). We also observed greater changes in gene expression in LPS activated BOMA cells than in non activated BOMA cells upon stimulation using Fh-ES. The results show possibility of using cell lines for in vitro research of bovine immune response against liver fluke, although this model still requires validation and further characterization.

In vitro differentiation of the murine macrophage cell line BDM-1 into osteoclast-like cells.

Endocrinology ◽  
1995 ◽  
Vol 136 (10) ◽  
pp. 4285-4292 ◽  
Author(s):  
J H Shin ◽  
A Kukita ◽  
K Ohki ◽  
T Katsuki ◽  
O Kohashi
Keyword(s):  
Cell Line ◽  
Murine Macrophage ◽  
In Vitro Differentiation ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line

Identification of tyrosine 706 in the kinase insert as the major colony-stimulating factor 1 (CSF-1)-stimulated autophosphorylation site in the CSF-1 receptor in a murine macrophage cell line

1990 ◽  
Vol 10 (6) ◽  
pp. 2991-3002
Author(s):  
P van der Geer ◽  
T Hunter
Keyword(s):  
Cell Line ◽  
Murine Macrophage ◽  
Colony Stimulating Factor ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Major Site ◽  
Murine Macrophage Cell Line ◽  
Stimulating Factor

The receptor for colony-stimulating factor 1 (CSF-1) is a ligand-activated protein-tyrosine kinase. It has been shown previously that the CSF-1 receptor is phosphorylated on serine in vivo and that phosphorylation on tyrosine can be induced by stimulation with CSF-1. We studied the phosphorylation of the CSF-1 receptor by using the BAC1.2F5 murine macrophage cell line, which naturally expresses CSF-1 receptors. Two-dimensional tryptic phosphopeptide mapping showed that the CSF-1 receptor is phosphorylated on several different serine residues in vivo. Stimulation with CSF-1 at 37 degrees C resulted in rapid phosphorylation on tyrosine at one major site and one or two minor sites. We identified the major site as Tyr-706. The identity of Tyr-706 was confirmed by mutagenesis. This residue is located within the kinase insert domain. There was no evidence that Tyr-973 (equivalent to Tyr-969 in the human CSF-1 receptor) was phosphorylated following CSF-1 stimulation. When cells were stimulated with CSF-1 at 4 degrees C, additional phosphotyrosine-containing phosphopeptides were detected and the level of phosphorylation of the individual phosphotyrosine-containing phosphopeptides was substantially increased. In addition, we show that CSF-1 receptors are capable of autophosphorylation at six to eight major sites in vitro.

Comparison of the In Vivo and In Vitro Proliferation of Monoblasts, Promonocytes, and the Macrophage Cell Line J774

1982 ◽  
pp. 175-187 ◽  
Author(s):  
R. van Furth ◽  
Th. J. L. M. Goud ◽  
J. W. M. van der Meer ◽  
A. Blussé van Oud Alblas ◽  
M. M. C. Diesselhoff-den Dulk ◽  
...  
Keyword(s):  
Cell Line ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
In Vitro Proliferation

scholarly journals Anti-inflammatory effects of Anredera cordifolia and Piper crocatum extracts on lipopolysaccharide-stimulated macrophage cell line

2017 ◽  
Vol 12 (1) ◽  
pp. 35 ◽  
Author(s):  
Dian Ratih Laksmitawati ◽  
Anisa Widyastuti ◽  
Nadia Karami ◽  
Ervi Afifah ◽  
Dwi Davidson Rihibiha ◽  
...  
Keyword(s):  
Cell Line ◽  
Tumor Necrosis ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Α Level ◽  
Necrosis Factor

<p class="Abstract">In this study, the anti-inflammatory potential of <em>Anredera </em>cordifolia and <em>Piper </em>crocatum extracts on lipopolysaccharide-induced murine macrophage cell line (RAW 264.7) was observed. Cell viability assay was performed with MTS assay. Parameters measured to determine the anti-inflammatory activity were interleukin-1β (IL-1β), tumor necrosis factor (TNF)-α, nitric oxide (NO) and IL-6. Both <em>A. </em>cordifolia and<em> P. </em>crocatum at concentration of 50 µg/mL in cell line resulted significant decrease in TNF-α level (250.3 and 242.5 pg/mL respectively). <em>A. </em>cordifolia showed significant decrease in IL-1β level at 50 µg/mL and IL-6 level at 10 µg/mL, whilst <em>P. </em>crocatum  showed significant decrease IL-1β level in three concentrations with lowest level at 50 µg/mL.<em> A. </em>cordifolia showed lowest decrease in NO level at 50 µg/mL but not comparable with normal cells, whilst <em>P. </em>crocatum showed significant decrease in NO level at 50 µg/mL. This research revealed that <em>A. </em>cordifolia and<em> P. </em>crocatum possess the anti-inflammatory potential indicated by the inhibitory activity of the inflammatory mediators including, TNF-α, IL-1β, IL-6, and NO.</p>

BIOAVAILABILITY OF BERYLLIUM OXIDE PARTICLES: AN IN VITRO STUDY IN THE MURINE J774A.1 MACROPHAGE CELL LINE MODEL

2005 ◽  
Vol 31 (3) ◽  
pp. 341-360 ◽  
Author(s):  
Gregory A. Day ◽  
Mark D. Hoover ◽  
Aleksandr B. Stefaniak ◽  
Robert M. Dickerson ◽  
Eric J. Peterson ◽  
...  
Keyword(s):  
Cell Line ◽  
In Vitro Study ◽  
Beryllium Oxide ◽  
Vitro Study ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Line Model ◽  
Cell Line Model ◽  
Oxide Particles

scholarly journals Antileishmanial Activities of Several Classes of Aromatic Dications

2002 ◽  
Vol 46 (3) ◽  
pp. 797-807 ◽  
Author(s):  
James J. Brendle ◽  
Abram Outlaw ◽  
Arvind Kumar ◽  
David W. Boykin ◽  
Donald A. Patrick ◽  
...  
Keyword(s):  
Cell Line ◽  
Leishmania Donovani ◽  
Pneumocystis Carinii ◽  
Antileishmanial Activity ◽  
Microbial Pathogens ◽  
Mouse Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Mouse Macrophage Cell Line

ABSTRACT Aromatic dicationic molecules possess impressive activity against a broad spectrum of microbial pathogens, including Pneumocystis carinii, Cryptosporidium parvum, and Candida albicans. In this work, 58 aromatic cations were examined for inhibitory activity against axenic amastigote-like Leishmania donovani parasites. In general, the most potent of the compounds were substituted diphenyl furan and thiophene dications. 2,5-Bis-(4-amidinophenyl)thiophene was the most active compound. This agent displayed a 50% inhibitory concentration (IC50) of 0.42 ± 0.08 μM against L. donovani and an in vitro antileishmanial potency 6.2-fold greater than that of the clinical antileishmanial dication pentamidine and was 155-fold more toxic to the parasites than to a mouse macrophage cell line. 2,4-Bis-(4-amidinopheny)furan was twice as active as pentamidine (IC50, 1.30 ± 0.21 μM), while 2,5-bis-(4-amidinopheny)furan and pentamidine were essentially equipotent in our in vitro antileishmanial assay. Carbazoles, dibenzofurans, dibenzothiophenes, and benzimidazoles containing amidine or substituted amidine groups were generally less active than the diphenyl furans and thiophenes. In all cases, aromatic dications possessing strong antileishmanial activity were severalfold more toxic to the parasites than to a cultured mouse macrophage cell line. These structure-activity relationships demonstrate the potent antileishmanial activity of several aromatic dications and provide valuable information for the future design and synthesis of more potent antiparasitic agents.

scholarly journals Characterisation of a New Human Alveolar Macrophage-Like Cell Line (Daisy)

Lung ◽  
2019 ◽  
Vol 197 (6) ◽  
pp. 687-698
Author(s):  
Laura R. Sadofsky ◽  
Yvette A. Hayman ◽  
Jesse Vance ◽  
Jorge L. Cervantes ◽  
Simon D. Fraser ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Line ◽  
Ex Vivo ◽  
Human Macrophage ◽  
Macrophage Cell Line ◽  
Binding Assays ◽  
Macrophage Cell ◽  
Mitogenic Stimulation ◽  
Human Alveolar Macrophage

Abstract Purpose There is currently no true macrophage cell line and in vitro experiments requiring these cells currently require mitogenic stimulation of a macrophage precursor cell line (THP-1) or ex vivo maturation of circulating primary monocytes. In this study, we characterise a human macrophage cell line, derived from THP-1 cells, and compare its phenotype to the THP-1 cells. Methods THP-1 cells with and without mitogenic stimulation were compared to the newly derived macrophage-like cell line (Daisy) using microscopy, flow cytometry, phagocytosis assays, antigen binding assays and gene microarrays. Results We show that the cell line grows predominantly in an adherent monolayer. A panel of antibodies were chosen to investigate the cell surface phenotype of these cells using flow cytometry. Daisy cells expressed more CD11c, CD80, CD163, CD169 and CD206, but less CD14 and CD11b compared with mitogen-stimulated THP-1 cells. Unlike stimulated THP-1 cells which were barely able to bind immune complexes, Daisy cells showed large amounts of immune complex binding. Finally, although not statistically significant, the phagocytic ability of Daisy cells was greater than mitogen-stimulated THP-1 cells, suggesting that the cell line is more similar to mature macrophages. Conclusions The observed phenotype suggests that Daisy cells are a good model of human macrophages with a phenotype similar to human alveolar macrophages.

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