scholarly journals Activity of four entomopathogenic nematode species against different developmental stages of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera, Chrysomelidae)

2009 ◽  
Vol 46 (1) ◽  
pp. 14-20 ◽  
Author(s):  
S. Trdan ◽  
M. Vidrih ◽  
L. Andjus ◽  
Ž. Laznik
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Developmental Stages ◽  
Control Method ◽  
Entomopathogenic Nematode ◽  
Nematode Species ◽  
Potato Production ◽  
Steinernema Feltiae ◽  
Potato Beetle ◽  
Different Temperatures

AbstractFour entomopathogenic nematode species (Steinernema feltiae, S. carpocapsae, Heterorhabditis bacteriophora, and H. megidis) were tested in a laboratory bioassay for the efficacy of these pathogens in controlling the larvae and adults of the Colorado potato beetle, Leptinotarsa decemlineata. The main aim of the study was to develop an efficient sustainable control method against the pest. With this we could develop a strategy of potato production with the intention of diminishing or even preventing the appearance of pest resistance to insecticides. The activity of these biological agents was assessed at three different temperatures (15, 20, and 25 °C) and three concentrations (200, 1000, and 2000 infective juveniles per individual). Mortality of three stages (young and old larvae and adults) was determined 2, 4, and 7 days after treatment. At 15 °C entomopathogenic nematodes showed the lowest efficacy against all insect stages. No significant differences in efficacy was determined at 20 and 25 °C as all nematodes caused prompt death of all stages. At all temperatures young larvae were most susceptible. However, when controlling overwintered adults for the purpose of preventing the mass appearance of Colorado potato beetle, we recommend an application of higher concentrations of S. feltiae suspension.

scholarly journals Damage potential and phenology of the Colorado potato beetle [Coleoptera: Chrysomelidae] on potato in southern Alberta

2005 ◽  
Vol 83 (2) ◽  
pp. 89-98 ◽  
Author(s):  
C. Noronha ◽  
G.M. Duke ◽  
M.S. Goettel
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Potato Production ◽  
Maximum Density ◽  
The Other ◽  
Damage Potential ◽  
Potato Beetle ◽  
Southern Alberta ◽  
Overwintering Mortality ◽  
Experimental Plots

The phenology and damage potential of the Colorado potato beetle (Leptinotarsa decemlineata) were studied in the potato producing area in southern Alberta. Experimental plots were established at Lethbridge in 1998, 1999 and 2000, and at Vauxhall in 1998 and 1999. At each site, one plot was protected against the beetle by application of insecticides while the other was "unprotected." Natural potato beetle populations quickly colonized unprotected plots each year. Overwintered adults appeared in plots by mid June with mean densities reaching between 0.3 and 0.6 per plant. Eggs were laid on young plants with mean densities reaching two egg masses per plant by late June. Maximum larval densities reached 9.5 per plant for each of 1st, 2nd and 3rd instars and 14 per plant for 4th instars. Maximum density for newly emerged adults was 57 per plant in mid-July at the 2000 Lethbridge unprotected plot. Defoliation was very low at the beginning of the season but increased sharply when 3rd and 4th instar populations peaked and continued to rise as new adults emerged. Maximum defoliation occurred at the Lethbridge plot in 2000 with 100% defoliation by 10 August. Total yields in all unprotected plots ranged from 10 to 40% lower than in the protected plots. Mean density of overwintering adults within potato plots was 76 beetles m-2 with a maximum of 232 m-2. Mean overwintering mortality was 22% and mean depth of overwintering adults was 12 cm, with 63% of the beetles collected at depths ≤ 10 cm. Our results indicate that the phenology of the beetle is similar to that reported in areas where population buildups were rapid and devastating soon after insecticide resistant populations appeared. Consequently the beetle must be considered as a serious threat to potato production in southern Alberta.

A Day in the Life: Identification of Developmentally Regulated MicroRNAs in the Colorado Potato Beetle (Leptinotarsa decemlineata; Coleoptera: Chrysomelidae)

2020 ◽  
Vol 113 (3) ◽  
pp. 1445-1454
Author(s):  
K F Wiebe ◽  
O O Elebute ◽  
C M R LeMoine ◽  
B J Cassone
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Developmental Stages ◽  
Early Stage ◽  
Control Strategies ◽  
Solanum Tuberosum L ◽  
Potato Beetle ◽  
Important Pest ◽  
Leptinotarsa Decemlineata Say ◽  
Potential Applications

Abstract The Colorado potato beetle (Leptinotarsa decemlineata (Say)) is an important pest of the cultivated potato (Solanum tuberosum (L.) [Solanales: Solanaceae]). With its broad resistance toward commonly used insecticides, it is clear that more sophisticated control strategies are needed. Due to their importance in insect development, microRNAs (miRNAs) represent a potential tool to employ in insect control strategies. However, most studies conducted in this area have focused on model species with well-annotated genomes. In this study, next-generation sequencing was used to catalogue the miRNAs produced by L. decemlineata across all eight stages of its development, from eggs to adults. For most stages, the length of miRNAs peaked between 21 and 22 nt, though it was considerably longer for the egg stage (26 nt). Global profiling of miRNAs revealed three distinct developmental clusters: 1) egg stage; 2) early stage (first, second, and third instar); and 3) late stage (fourth instar, prepupae, pupae, and adult). We identified 86 conserved miRNAs and 33 bonafide novel miRNAs, including stage-specific miRNAs and those not previously identified in L. decemlineata. Most of the conserved miRNAs were found in multiple developmental stages, whereas the novel miRNAs were often stage specific with the bulk identified in the egg stage. The identified miRNAs have a myriad of putative functions, including growth, reproduction, and insecticide resistance. We discuss the putative roles of some of the most notable miRNAs in the regulation of L. decemlineata development, as well as the potential applications of this research in Colorado potato beetle management.

scholarly journals Contact Toxicities of Oxygenated Monoterpenes to Different Populations of Colorado Potato Beetle, Leptinotarsa Decemlineata Say (Coleoptera: Chrysomelidae)

2011 ◽  
Vol 51 (3) ◽  
pp. 225-233 ◽  
Author(s):  
Safaei Mahdi ◽  
Jafarnia Sasan ◽  
Khosroshahi Sara
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Developmental Stages ◽  
Linalyl Acetate ◽  
Potential Control ◽  
Oxygenated Monoterpenes ◽  
Potato Beetle ◽  
Leptinotarsa Decemlineata Say ◽  
Different Populations ◽  
Different Doses

Contact Toxicities of Oxygenated Monoterpenes to Different Populations of Colorado Potato Beetle,Leptinotarsa DecemlineataSay (Coleoptera: Chrysomelidae)In the present study, 12 pure oxygenated monoterpenes at 2 different doses were tested for their toxicity against second and third instar larvae and adults of three different populations of Colorado potato beetle (Leptinotarsa decemlineataSay). Some of tested compounds were found to be toxic to larvae and adults, but the degree of toxicity was variable. The mortality range was 20-100%. In general, fenchone, linalool, citronella and menthone showed a strong toxicity against the tested developmental stages; camphor, carvone and linalyl acetate showed moderate toxicity against larvae and adults of Colorado potato beetle and some compounds like fenchol, isomenthol, menthol, nerol and neryl acetate showed the least or no toxicity against the tested developmental stages ofL. decemlineata.Another important result was that although the tested populations of Colorado potato beetle showed some resistance to Endosulfan (50% WP), there was no resistance to tested oxygenated monoterpenes. The present results indicate that some of these compounds can be used as potential control agents against both larvae and adults of Colorado potato beetle.

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