Extraction and analysis of ellagic acid from novel complex sources

2008 ◽  
Vol 62 (4) ◽  
Author(s):  
Antonio Aguilera-Carbo ◽  
Christopher Augur ◽  
Lilia Prado-Barragan ◽  
Cristóbal Aguilar ◽  
Ernesto Favela-Torres
Keyword(s):  
Ellagic Acid ◽  
High Performance ◽  
Punica Granatum ◽  
Reversed Phase ◽  
Semiarid Region ◽  
Photodiode Array Detection ◽  
Dragon’S Blood ◽  
Turnera Diffusa ◽  
Photodiode Array ◽  
Array Detection

AbstractEllagic acid (EA) was quantified by reversed-phase high-performance liquid chromatography (RPHPLC) coupled with photodiode array detection (DAD) in five fine-powdered plants collected from the semiarid region of Mexico. Samples analysed included Jatropha dioica branches (Dragon’s blood), Euphorbia antisyphyllitica branches (Candelilla), Turnera diffusa Willd leaves (Damiana), Flourensia cernua leaves (hojasén) and Punica granatum husk (pomegranate) at two maturity stages (“turning” or intermediate and maturated fruit, considered as positive controls). The results demonstrated high EA concentrations in all tested samples which are novel sources of this natural antioxidant. The method developed for the EA analysis is fast and it showed an excellent linearity range, repeatability, intra-and inter-day precision and accuracy with respect to the methods reported for the EA analysis.

Analysis of supplemented vitamin K1(20) in serum microsamples by solid-phase extraction and narrow-bone HPLC with multichannel ultraviolet detection.

1989 ◽  
Vol 35 (7) ◽  
pp. 1288-1292 ◽  
Author(s):  
E M Kirk ◽  
A F Fell
Keyword(s):  
Solid Phase Extraction ◽  
High Performance ◽  
Solid Phase ◽  
Reversed Phase ◽  
Phase Extraction ◽  
Calibration Data ◽  
Vitamin K1 ◽  
Photodiode Array Detection ◽  
Photodiode Array ◽  
Array Detection

Abstract A sensitive method for the determination of vitamin K1(20) in serum microsamples (50 microL) has been developed, utilizing solid-phase extraction with C8 Bond-Elut columns and reversed-phase narrow-bore high-performance liquid chromatography [2.1 mm (i.d.), column] with a nonaqueous eluent. Recovery from serum (49 ng/mL) was 76% (n = 2). Peak homogeneity was assessed by photodiode array detection with absorbance ratio, spectral normalization, and transformation to the first- and second-derivative chromatograms. Calibration data at 248 nm over two ranges (20-200 ng/mL, 200-4000 ng/mL) varied linearly with concentration and were suitable for studies of vitamin K1 supplementation. By comparison with conventional columns, sensitivity was increased twofold.

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