scholarly journals Comparison of classic and derivative UV spectrophotometric methods for determination of dextromethorphani hydrobromidum

2012 ◽  
Vol 59 (1) ◽  
pp. 22-29
Author(s):  
J. Drozd
Keyword(s):  
Pharmaceutical Preparations ◽  
Derivative Spectrophotometry ◽  
Order Derivative ◽  
Third Order ◽  
Fast Determination ◽  
Spectrophotometric Methods ◽  
Calibration Curves

Comparison of classic and derivative UV spectrophotometric methods for determination of dextromethorphani hydrobromidumA method for the fast determination of dextromethorphani hydrobromidum in pharmaceutical preparations by classic spectrophotometry - zero and first-, second- and third-order derivative spectrophotometry, using "peak - peak" (P - P) and "peak - zero" (P - O) measurements has been performed. The calibration curves are linear within the concentration range of 1.0 - 25.0 μg ml-1 for dextromethorphani hydrobromidum. The procedure is simple, rapid and the results are reliable.

scholarly journals High-performance liquid chromatography and derivative spectrophotometry for simultaneous determination of pravastatin and fenofibrate in the dosage form

2014 ◽  
Vol 64 (4) ◽  
pp. 433-446 ◽  
Author(s):  
Mohamed M. Hefnawy ◽  
Mostafa S. Mohamed ◽  
Mohammed A. Abounassif ◽  
Amer M. Alanazi ◽  
Gamal A. E. Mostafa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Particle Diameter ◽  
Second Order ◽  
Derivative Spectrophotometry ◽  
Order Derivative ◽  
Calibration Curves

Abstract High performance liquid chromatography (HPLC) and second-order derivative spectrophotometry have been used for simultaneous determination of pravastatin (PS) and fenofibrate (FF) in pharmaceutical formulations. HPLC separation was performed on a phenyl HYPERSIL C18 column (125 mm × 4.6 mm i.d., 5 μm particle diameter) in the isocratic mode using a mobile phase acetonitrile/0.1 % diethyl amine (50:50, V/V, pH 4.5) pumped at a flow rate of 1.0 mL min-1. Measurement was made at 240 nm. Both drugs were well resolved on the stationary phase, with retention times of 2.15 and 5.79 min for PS and FF, respectively. Calibration curves were linear (R = 0.999 for PS and 0.996 for FF) in the concentration range of 5-50 and 20-200 µg mL-1 for PS and FF, respectively. Pravastatin and fenofibrate were quantitated in combined preparations also using the second-order derivative response at 237.6 and 295.1 nm for PS and FF, respectively. Calibration curves were linear, with the correlation coefficient R = 0.999 for pravastatin and fenofibrate, in the concentration range of 5-20 and 3-20 µg mL-1 for PS and FF, respectively. Both methods were fully validated and compared, the results confirmed that they were highly suitable for their intended purpose.

Third Order Derivative Spectrophotometry Determination of Mercury (II) as the Ternary Complex with Iodide and Pyronine G

1992 ◽  
Vol 25 (10) ◽  
pp. 1941-1959 ◽  
Author(s):  
Saji Mathew ◽  
Rugmini Sukumar ◽  
T. Prasada Rao ◽  
A. D. Damodaran
Keyword(s):  
Ternary Complex ◽  
Derivative Spectrophotometry ◽  
Order Derivative ◽  
Third Order

Rapid Determination of Amphotericin B in Serum and Urine by Third-Order Derivative Spectrophotometry

1996 ◽  
Vol 85 (4) ◽  
pp. 402-406 ◽  
Author(s):  
Nickos A. Botsoglou ◽  
Dimitrios J. Fletouris ◽  
George E. Papageorgiou ◽  
Panagiota Florou-Paneri ◽  
Antonios J. Mantis
Keyword(s):  
Amphotericin B ◽  
Rapid Determination ◽  
Derivative Spectrophotometry ◽  
Order Derivative ◽  
Third Order ◽  
Serum And Urine

Colorimetric Determination of Amoxicillin in Pure and some Pharmaceutical Formulations Via Reaction with Potassium Permanganate as Oxidant Reagent

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Abbas Shebeeb Al-kadumi ◽  
Sahar Rihan Fadhel ◽  
Mohammed Abdullah Ahmed ◽  
Luma Amer Musa
Keyword(s):  
Potassium Permanganate ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Atomic Emission ◽  
Basic Medium ◽  
Photometric Method ◽  
Colorimetric Determination ◽  
Spectrophotometric Methods ◽  
Label Claim

We proposed two simple, rapid, and convenient spectrophotometric methods are described for the determination of Amoxicillin in bulk and its pharmaceutical preparations. They are based on the measurement of the flame atomic emission of potassium ion (in first method) and colorimetric determination of the green colored solution for manganite ion at 610 nm formed after reaction of Amoxicillin with potassium permanganate as oxidant agent (in the second method) in basic medium. The working conditions of the methods were investigated and optimized. Beer's law plot showed a good correlation in the concentration range of 5-45 μg/ml. The detection limits and relative standared deviations were (2.573, 2.814 μg/ml) (2.137, 2.498) for the flame emission photometric method and (1.844, 2.016 μg/ml) (1.645,1.932) for colorimetric methods for capsules and suspensions respectively. The methods were successfully applied to the determination of Amoxicillin in capsules and suspensions, and the obtained results were in good agreement with the label claim. No interference was observed from the commonly encountered additives and expectancies.

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