scholarly journals Alterations in Deciduous Dental Pulp Cells Cultured with Serum-free Medium

2015 ◽  
Vol 24 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Kyoko Harada ◽  
Saki Kawai ◽  
Xu Wen-an ◽  
Xu liang ◽  
Mie Sonomoto ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Pulp Cells ◽  
Cells Cultured

scholarly journals Characterization of human dental pulp cells grown in chemically defined serum‑free medium

2018 ◽  
Author(s):  
Sakiko Fujii ◽  
Katsumi Fujimoto ◽  
Noriko Goto ◽  
Yoshimitsu Abiko ◽  
Asayo Imaoka ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

Expression of Multiple Stem Cell Markers in Dental Pulp Cells Cultured in Serum-free Media

2010 ◽  
Vol 36 (7) ◽  
pp. 1139-1144 ◽  
Author(s):  
Thais Miyuki Hirata ◽  
Nikolay Ishkitiev ◽  
Ken Yaegaki ◽  
Bogdan Calenic ◽  
Hiroshi Ishikawa ◽  
...  
Keyword(s):  
Stem Cell ◽  
Dental Pulp ◽  
Stem Cell Markers ◽  
Dental Pulp Cells ◽  
Serum Free ◽  
Cell Markers ◽  
Pulp Cells ◽  
Free Media ◽  
Cells Cultured

scholarly journals Evaluation of growth, stemness, and angiogenic properties of dental pulp stem cells cultured in cGMP xeno-/serum-free medium

2019 ◽  
Vol 380 (1) ◽  
pp. 93-105 ◽  
Author(s):  
Chengjuan Qu ◽  
Maria Brohlin ◽  
Paul J Kingham ◽  
Peyman Kelk
Keyword(s):  
Stem Cells ◽  
Human Serum ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
In Vitro Assays ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Cells Cultured

AbstractThis study was aimed to investigate the effects of cGMP xeno-/serum-free medium (XSF, Irvine Scientific) on the properties of human dental pulp stem cells (DPSCs). DPSCs, from passage 2, were cultured in XSF or fetal bovine serum (FBS)-supplemented medium, and sub-cultured up to passage 8. Cumulative population doublings (PDs) and the number of colony-forming-units (CFUs) were determined. qRT-PCR, ELISA, and in vitro assays were used to assess angiogenic capacity. Flow cytometry was used to measure CD73, CD90, and CD105 expression. Differentiation into osteo-, adipo-, and chondrogenic cell lineages was performed. DPSCs showed more elongated morphology, a reduced rate of proliferation at later passages, and lower CFU counts in XSF compared with FBS. Expression of angiogenic factors at the gene and protein levels varied in the two media and with passage number, but cells grown in XSF had more in vitro angiogenic activity. The majority of early and late passage DPSCs cultured in XSF expressed CD73 and CD90. In contrast, the percentage of CD105 positive DPSCs in XSF medium was significantly lower with increased passage whereas the majority of cells cultured in FBS were CD105 positive. Switching XSF-cultured DPSCs to medium supplemented with human serum restored the expression of CD105. The tri-lineage differentiation of DPSCs cultured under XSF and FBS conditions was similar. We showed that despite reduced CD105 expression levels, DPSCs expanded in XSF medium maintained a functional MSC phenotype. Furthermore, restoration of CD105 expression is likely to occur upon in vivo transplantation, when cells are exposed to human serum.

Effect of Nifedipine on the Differentiation of Human Dental Pulp Cells Cultured with Mineral Trioxide Aggregate

2013 ◽  
Vol 39 (6) ◽  
pp. 801-805 ◽  
Author(s):  
Su-Mi Woo ◽  
Yun-Chan Hwang ◽  
Hoi-Soon Lim ◽  
Nam-Ki Choi ◽  
Sun-Hun Kim ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Mineral Trioxide Aggregate ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Cells Cultured

Vectorial release of carcinoembryonic antigen induced by IFN-γ in human colon cancer cells cultured in serum-free medium

1991 ◽  
Vol 27 (5) ◽  
pp. 599-604 ◽  
Author(s):  
Stephen Baghdiguian ◽  
Bernard Verrier ◽  
Monique Roccabianca ◽  
Gilbert Pommier ◽  
Jacques Marvaldi ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Human Colon ◽  
Serum Free Medium ◽  
Colon Cancer Cells ◽  
Free Medium ◽  
Human Colon Cancer ◽  
Serum Free ◽  
Ifn Γ ◽  
Human Colon Cancer Cells ◽  
Cells Cultured

scholarly journals Enhanced Differentiation of Dental Pulp Cells Cultured on Microtubular Polymer Scaffolds In Vitro

2017 ◽  
Vol 3 (2) ◽  
pp. 94-105 ◽  
Author(s):  
Morteza Haeri ◽  
Karen Sagomonyants ◽  
Mina Mina ◽  
Liisa T. Kuhn ◽  
A. Jon Goldberg
Keyword(s):  
Dental Pulp ◽  
Polymer Scaffolds ◽  
Dental Pulp Cells ◽  
Pulp Cells ◽  
Cells Cultured

scholarly journals Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation

2020 ◽  
Author(s):  
Ming Yan ◽  
Ola A. Nada ◽  
Ralf Smeets ◽  
Martin Gosau ◽  
Reinhard E. Friedrich ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Cells Cultured
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