Characterization of human dental pulp cells grown in chemically defined serum‑free medium

In this study, hydrogels based on chitosan cross-linked by glyoxal have been investigated for potential medical applications. Hydrogels were loaded with tannic acid at different concentrations. The thermal stability and the polyphenol-releasing rate were determined. For a preliminary assessment of the clinical usefulness of the hydrogels, they were examined for blood compatibility and in the culture of human dental pulp cells (hDPC). The results showed that after immersion in a polyphenol solution, chitosan/glyoxal hydrogels remain nonhemolytic for erythrocytes, and we also did not observe the cytotoxic effect of hydrogels immersed in tannic acid (TA) solutions with different concentration. Tannic acid was successfully released from hydrogels, and its addition improved material thermal stability. Thus, the current findings open the possibility to consider such hydrogels in clinics.

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In vitro characterization of human dental pulp cells: various isolation methods and culturing environments

Cell and Tissue Research ◽

10.1007/s00441-005-0117-9 ◽

2006 ◽

Vol 324 (2) ◽

pp. 225-236 ◽

Cited By ~ 118

Author(s):

George T.-J. Huang ◽

Wataru Sonoyama ◽

James Chen ◽

Sang Hyuk Park

Keyword(s):

Dental Pulp ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

In Vitro Characterization ◽

Isolation Methods ◽

Human Dental Pulp ◽

Pulp Cells

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Characterization of Human Dental Pulp Cells from Supernumerary Teeth by Using Flow Cytometry Analysis

THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY ◽

10.5933/jkapd.2019.46.3.337 ◽

2019 ◽

Vol 46 (3) ◽

pp. 337-342

Author(s):

Yonsook You ◽

Jongbin Kim ◽

Jisun Shin ◽

June-Haeng Lee ◽

Jongsoo Kim

Keyword(s):

Flow Cytometry ◽

Dental Pulp ◽

Dental Pulp Cells ◽

Flow Cytometry Analysis ◽

Supernumerary Teeth ◽

Human Dental Pulp Cells ◽

Human Dental Pulp ◽

Pulp Cells

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Expression, Purification, and Characterization of a Dentin Phosphoprotein Produced by Escherichia coli, and Its Odontoblastic Differentiation Effects on Human Dental Pulp Cells

The Protein Journal ◽

10.1007/s10930-012-9430-9 ◽

2012 ◽

Vol 31 (6) ◽

pp. 504-510 ◽

Cited By ~ 1

Author(s):

Ye-Rang Yun ◽

Eunyi Jeon ◽

Sujin Lee ◽

Wonmo Kang ◽

Sang-Gi Kim ◽

...

Keyword(s):

Escherichia Coli ◽

Dental Pulp ◽

Dental Pulp Cells ◽

Purification And Characterization ◽

Human Dental Pulp Cells ◽

Odontoblastic Differentiation ◽

Dentin Phosphoprotein ◽

Human Dental Pulp ◽

Pulp Cells

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Preparation and characterization of Jagged1-bound fibrinogen-based microspheres and their cytotoxicity against human dental pulp cells

Journal of Biomaterials Applications ◽

10.1177/0885328219898579 ◽

2020 ◽

Vol 34 (8) ◽

pp. 1105-1113

Author(s):

Chawan Manaspon ◽

Lawan Boonprakong ◽

Thantrira Porntaveetus ◽

Thanaphum Osathanon

Keyword(s):

Dental Pulp ◽

Biomedical Application ◽

Binding Capacity ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Significant Difference ◽

Human Dental Pulp ◽

Pulp Cells

Surface immobilization of Jagged1 promotes odonto/osteogenic differentiation in human dental pulp cells. On the contrary, soluble Jagged1 fails to activate target gene expression of Notch signaling which is important for differentiation of human dental pulp cells. Hence, Jagged1 delivery system is indeed required for transportation of immobilized Jagged1 to promote odontogenic differentiation of human dental pulp cells in vivo. The present study described the preparation and characterization of Jagged1-bound fibrinogen-based microspheres. Water-in-oil emulsion technique was employed to prepare fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres. The average size of fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres was 213.9 ± 35.9 and 199.9 ± 41.9 µm, respectively. These microspheres did not alter the human dental pulp cells’ cell viability. Human dental pulp cells were able to attach and spread on these microspheres. Jagged1 was conjugated on microspheres using 1-ethyl-3-(3-dimethylamino) propyl carbodiimide/N-hydroxysuccinimide. Binding capacity of Jagged1 on both fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres ranged from 25.8 ± 6.0 to 35.6 ± 9.1%. There was no significant difference in the size of microspheres between before and after Jagged1 conjugation process. In conclusion, fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres could be utilized as the alternative biomaterials for Jagged1 delivery for future biomedical application.

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