scholarly journals Bazı Turunçgil Anaçlarının Klasik ve Yeni Nesil Doku Kültürü Teknikleri ile Mikroçoğaltımı

2019 ◽  
Vol 7 (9) ◽  
pp. 1469
Author(s):  
Melike Cengiz ◽  
Yıldız Aka Kaçar
Keyword(s):  
Culture Medium ◽  
Woody Plant ◽  
Naphthaleneacetic Acid ◽  
Solid Culture ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Rooting Medium ◽  
Solid Culture Medium ◽  
Isopentenyl Adenine ◽  
Bioreactor System

In this study, micropropagation and rooting of ‘Tuzcu 31-31 sour orange’ and ‘C-35 citrange’ citrus rootstocks were conducted by comparing with Plantform temporary immersion bioreactor system and traditional solid culture. Murashige and Skoog Medium (MS) and Woody Plant Medium (WPM) supplemented with 6-Benzylaminopurine (BAP) (0, 1.0, 2.0 mg L-1), Kinetin (KIN) (0, 0.5, 1.0 mg L-1) and 2-Isopentenyl adenine (2IP) (0, 1.0, 2.0 mg L-1) were used in solid culture experiments. For solid culture rooting experiments, MS, ½ MS and WPM media supplemented with different concentrations of 1-Naphthaleneacetic acid (NAA) (0, 0.5, 1.0, 2.0 mg L-1) and Indole-3-butyric acid (IBA) (0, 0.5, 1.0, 2.0 mg L-1) were used. In both genotypes, the best micropropagation and rooting results were obtained from MS medium containing 2.0 mg L-1 BAP and ½ MS nutrient medium containing 0.5 mg L-1 NAA, respectively. Plantform bioreactor system was studied with the best medium content determined for micropropagation and rooting. As a result of the study, Plantform system gave better results in terms of plant quality in the micropropagation medium for both genotypes. Plantform system in rooting medium was found to be more advantageous than solid culture medium. As a result of the screening with SSR markers, it was determined that there was no somaclonal variation in the plants micropropagated and rooted in Plantform system.

scholarly journals Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry

2019 ◽  
Vol 41 (1) ◽  
Author(s):  
Samila Silva Camargo ◽  
Leo Rufato ◽  
Maicon Magro ◽  
André Luiz Kulkamp de Souza
Keyword(s):  
Liquid Medium ◽  
Culture Medium ◽  
Culture Media ◽  
Time Factors ◽  
Efficient Technique ◽  
Control Treatment ◽  
Solid Culture ◽  
Temporary Immersion ◽  
Solid Culture Medium

Abstract The in vitro propagation technique via temporary immersion bioreactors is a tool that, through the culture in a liquid medium, allows an increase in the efficiency of seedling production. Several researches with the strawberry crop have shown greater efficiency of the system compared to the conventional process of micropropagation in solid medium. In this sense, the objective herein was to establish a protocol of multiplication and rooting of the ‘Pircinque’ strawberry, in temporary immersion bioreactors. Two distinct and independent studies were carried out, characterized by the multiplication and rooting stages of strawberry explants, newly introduced and registered in Brazil. Two culture media (MS and KNOP) were studied and, as a control treatment, the growth of the explants in solid culture medium was evaluated with the addition of 5 g L-1 of agar. Different immersion times of the culture medium were explored: five or eight times a day, for 15 minutes. The study was composed of the culture medium and immersion time factors, as well as the control (solid) treatment. It was verified that the use of temporary immersion bioreactors system is an efficient technique for the multiplication and rooting of explants of strawberry cv. Pircinque, when compared to the conventional method of micropropagation with the use of solid culture medium, making it possible to optimize the production of seedlings in biofactories. The MS liquid medium, in contact with explants of ‘Pircinque’ strawberry five times a day, increased the growth of the aerial part and the root system.

Use of temporary immersion bioreactors and solid culture medium in the in vitro propagation of pear rootstocks

2021 ◽  
pp. 113-120
Author(s):  
A.L. Arruda ◽  
F.R. Nerbass ◽  
A.A. Kretzschmar ◽  
L. Rufato ◽  
A.J. Posser ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Solid Culture ◽  
Temporary Immersion ◽  
Solid Culture Medium

scholarly journals THE USE OF CONTINUOUS, TEMPORARY IMMERSION BIOREACTOR SYSTEM AND SEMISOLID CULTURE MEDIUM FOR THE PRODUCTION Of Eucalyptus camaldulensis CLONES

2016 ◽  
Vol 26 (4) ◽  
pp. 1211 ◽  
Author(s):  
Evânia Galvão Mendonça ◽  
Vanessa Cristina Stein ◽  
Humberto Henrique De Carvalho ◽  
Breno Régis Santos ◽  
Luiz Alberto Beijo ◽  
...  
Keyword(s):  
Culture Medium ◽  
Eucalyptus Camaldulensis ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Bioreactor System

A micropropagação em sistemas de biorreatores é considerada como uma forma de reduzir os custos de produção por meio do escalonamento de automatização do processo. O objetivo desse trabalho foi desenvolverum protocolo eficiente de produção de mudas de Eucalyptus camaldulensis em diferentes tipos de sistema, incluindo biorreator de imersão continua e temporária. Para isso, meristemas apicais (1 mm) e meristemas apicais com tecido adjacente (2,5 mm) foram usados como explantes iniciais. Esses tecidos foram cultivados, por 60 dias, em meio de cultura suplementado com 1 mg L-1 de ácido indolacético (AIA) e 0.32 mg L-1 de benzilaminopurina (BAP). Após 60 dias, os meristemas com tecidos adjacentes foram transferidos para biorreatores de imersão contínua ou temporária e mantidos no escuro ou sob condições controladas de luminosidade. Para verificar o efeito da fonte de explante na multiplicação em biorreator foram testados explantes subcultivados de meristemas multiplicados em meio de cultura semissólido e meristemas multiplicados em biorreator de imersão contínua e mantidos no escuro. Despois de estabelecer esses parâmetros, os experimentos de multiplicação foram realizados em biorreatores de imersão contínua e temporária. Os explantes multiplicados foram enraizados em meio de cultura MS suplementado com 0, 2, 4, 8 e 20 mg L-1 de ácido indolbutírico (AIB) e mantidos no escuro ou sob condições controladas de luminosidade. Depois do enraizamento as plantas foram aclimatizadas em câmara de nebulização. Os meristemas com tecidos adjacentes favoreceram um maior número de gemas/explantes. O biorreator de imersão contínua e mantido no escuro promoveu maior número de brotações e maior taxa de multiplicação e o melhor enraizamento ocorreram no meio de cultura isento de auxina, mantido no escuro por 15 dias ou o meio de cultura suplementado com auxina, mantido na luz apresentando 100% de enraizamento. A aclimatização do Eucalyptus camaldulensis foi eficiente com taxa de sobrevivência de 76%. Portanto, foi possível desenvolver um método eficiente de micropropagação em biorreator para a produção de mudas Eucalyptus camaldulensis em larga escala.

scholarly journals Sucrose concentration and volume of liquid medium on the in vitro growth and development of blackberry cv. Tupy in temporary immersion systems

2019 ◽  
Vol 43 ◽  
Author(s):  
Ricardo Antônio Ayub ◽  
Jessé Neves dos Santos ◽  
Luiz Antônio Zanlorensi Junior ◽  
Daurimar Mendes da Silva ◽  
Tereza Cristina de Carvalho ◽  
...  
Keyword(s):  
Growth And Development ◽  
Culture Medium ◽  
Sucrose Concentration ◽  
Seedling Production ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Number Of Leaves ◽  
Bioreactor System ◽  
Temporary Immersion Systems

ABSTRACT Micropropagation of small fruits such as blackberry has been employed due to the need to obtain plants with high phytosanitary quality. Bioreactor technology has been used to improve efficiency in seedling production. Thus, the objective of this work was to evaluate the best culture medium volume and sucrose concentration for blackberry micropropagation in a temporary immersion bioreactor. In vitro blackberry shoots were segmented containing two buds and an internode (1.0 cm) and placed into MS medium supplemented with inositol (0.1 g L-1), BAP (1 mg L-1) and sucrose (10, 20, 30 or 40 g L-1) at different medium volumes (150, 175 and 200 mL). The total length, number of leaves, number of shoots, and number of hyperhydric shoots were evaluated 56 days after start of the project. For blackberry development and propagation in a bioreactor system, the best results were shown at a medium volume of 175 ml and a sucrose concentration of 20 g L-1.

Efficient production of virus-free apple plantlets using the temporary immersion bioreactor system

2020 ◽  
Vol 61 (4) ◽  
pp. 779-785
Author(s):  
Na-Young Kim ◽  
Ho-Dong Hwang ◽  
Jin-Ho Kim ◽  
Bo-Min Kwon ◽  
Daeil Kim ◽  
...  
Keyword(s):  
Efficient Production ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Bioreactor System
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