Effects of single and binary applications of bisphenol A and NaCl on Ceratopyllum demersum

Determining the physiological effects of single and binary applications of BPA and NaCl on the Ceratophyllum demersum L., which is a rootless submerged aquatic macrophyte, is the aim of the present study. For this purpose, the macrophyte was collected from a local pond that was not exposed to any pollution. Macrophytes were acclimatized in 10% nutrient solution for 14 days, than they were treated with 17.2 mg/L BPA; 10 and 100 mM NaCl; 17.2 mg/L BPA plus 10 mM NaCl and 17.2 mg/L BPA plus 100 mM NaCl. The macrophyte without added BPA or/and NaCl served as control (without treatment). Increasing concentrations of NaCl induced a progressive accumulation of Na in the macrophyte tissues. On the other hand, BPA application partially reduced Na uptake. Nutrient uptake was affected differently by the applications. The contents of some nutrients such as Cu, Zn, Mn, K and Mg were generally reduced, whereas the Fe and Ca contents were increased. In general, increases in contents of total carbohydrate, total phenolic and non-protein sulfhydryl groups were found, when compared to control. Protein and photosynthetic pigment contents, on the contrary to these, were decreased. According to findings, the increase in H2O2 and MDA levels showed that single and combined applications of BPA and NaCl in C. demersum tissues induced oxidative stress.

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Physiological response of Moringa oleifera exposed to bisphenol A

Botanica Serbica ◽

10.2298/botserb2002183c ◽

2020 ◽

Vol 44 (2) ◽

pp. 183-189

Author(s):

Gulcan Cinar ◽

Muhittin Dogan

Keyword(s):

Bisphenol A ◽

Moringa Oleifera ◽

Phenolic Content ◽

Photosynthetic Pigment ◽

Total Phenolic ◽

Total Carbohydrate ◽

Total Carbohydrate Content ◽

Malondialdehyde Content ◽

Stems And Leaves ◽

Root Tissues

The physiological and morphological effects of different concentrations of bisphenol A (BPA) on Moringa oleifera seedlings were determined in this study. Significant chlorosis and abscission were observed in leaves exposed to 50 mg/L of BPA. Photosynthetic pigment levels were affected differently by varying doses of BPA. Although the total carbohydrate content of seedling parts was increased by BPA, protein content was lowered by it, except in the case of roots at 1.5 mg/L of BPA. However, it was determined that the content of nonprotein sulphhydryl groups of seedling parts did not change significantly. The total phenolic content of root tissues showed an insignificant change; however, it was found that phenolic content increased in the stems and leaves following application of BPA. The content of hydrogen peroxide (H2O2) in seedling tissues increased with increasing concentrations of BPA. Statistical analysis indicated that H2O2 content was significantly correlated with malondialdehyde content. These results clearly show that the application of BPA causes oxidative stress in seedling tissues.

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A reaction involving protein sulfhydryl groups, a bound spin-label, and K3Fe(CN)6 as a probe of sulfhydryl proximity in myosin

Biochemistry ◽

10.1021/bi00542a006 ◽

1980 ◽

Vol 19 (1) ◽

pp. 33-39 ◽

Cited By ~ 30

Author(s):

Philip Graceffa ◽

John C. Seidel

Keyword(s):

Spin Label ◽

Sulfhydryl Groups ◽

Protein Sulfhydryl ◽

Protein Sulfhydryl Groups

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Interaction of surfactant mixtures with reactive oxygen and nitrogen species

Journal of Applied Physiology ◽

10.1152/jappl.1995.78.5.1800 ◽

1995 ◽

Vol 78 (5) ◽

pp. 1800-1805 ◽

Cited By ~ 21

Author(s):

J. Cifuentes ◽

J. Ruiz-Oronoz ◽

C. Myles ◽

B. Nieves ◽

W. A. Carlo ◽

...

Keyword(s):

Lung Surfactant ◽

Alveolar Epithelium ◽

Reactive Species ◽

Surfactant Mixtures ◽

Surfactant Deficiency ◽

High Lipid ◽

Fenton Reagents ◽

Protein Sulfhydryl ◽

Nitrogen Reactive Species ◽

Protein Sulfhydryl Groups

Increased concentrations of partially reduced oxygen and nitrogen reactive species damage the alveolar epithelium and either cause or exacerbate surfactant deficiency. For this reason, there is a quest to identify surfactant replacement mixtures, which in addition to repleting depleted surfactant stores can also reduce the steady-state concentrations of reactive species in the alveolar space. Herein, we evaluated the ability of natural lung surfactant (NLS) and two mixtures (Exosurf and Survanta) used clinically for the correction of surfactant deficiency to scavenge hydroxyl radical-type species (.OH), generated either by the decomposition of peroxynitrite or by Fenton reagents (FeCl3 + H2O2). Exosurf or Survanta decreased .OH only when present at high lipid concentrations (6.5 mM). On the other hand, 40 microM of NLS decreased .OH concentrations from 75 +/- 2 to 53 +/- 2 microM (P < 0.05), most likely because of the interaction of .OH with protein sulfhydryl groups. Similarly, 40 microM of NLS incubated with a bolus of H2O2 (400 microM) decreased the H2O2 concentration in the supernatant by approximately 50%, due to the presence of catalase-type activity. In contrast to NLS, neither Exosurf nor Survanta scavenged H2O2, even when present at millimolar lipid concentrations. We concluded that Exosurf and Survanta contain limited antioxidant activity compared with NLS.

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A simple method for stabilizing protein-sulfhydryl groups during SDS-gel electrophoresis

Analytical Biochemistry ◽

10.1016/0003-2697(78)90792-3 ◽

1978 ◽

Vol 86 (2) ◽

pp. 655-664 ◽

Cited By ~ 162

Author(s):

Leslie C. Lane

Keyword(s):

Gel Electrophoresis ◽

Sulfhydryl Groups ◽

Simple Method ◽

Protein Sulfhydryl ◽

Protein Sulfhydryl Groups

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Gastroprotective effect of the alkaloid boldine: Involvement of non-protein sulfhydryl groups, prostanoids and reduction on oxidative stress

Chemico-Biological Interactions ◽

10.1016/j.cbi.2020.109166 ◽

2020 ◽

Vol 327 ◽

pp. 109166

Author(s):

Thaise Boeing ◽

Luisa Natália Bolda Mariano ◽

Ana Caroline dos Santos ◽

Bianca Tolentino ◽

Angela Cadorin Vargas ◽

...

Keyword(s):

Oxidative Stress ◽

Sulfhydryl Groups ◽

Gastroprotective Effect ◽

Protein Sulfhydryl ◽

Protein Sulfhydryl Groups

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Lipopolysaccharide from Escherichia coli prevents indomethacin-induced gastric damage in rats: role of non-protein sulfhydryl groups and leukocyte adherence

Inflammation Research ◽

10.1007/s00011-009-0040-8 ◽

2009 ◽

Vol 58 (10) ◽

pp. 717-723 ◽

Cited By ~ 4

Author(s):

Antoniella Souza Gomes ◽

Henrique Paula Lemos ◽

Jand Venes Rolim Medeiros ◽

Fernando Queiroz Cunha ◽

Marcellus Henrique Loiola Ponte Souza

Keyword(s):

Escherichia Coli ◽

Sulfhydryl Groups ◽

Gastric Damage ◽

Leukocyte Adherence ◽

Protein Sulfhydryl ◽

Protein Sulfhydryl Groups

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Amifostine (Wr-2721) Prevents Indomethacin-Induced Gastric Damage in Rats: Role of Non-Protein Sulfhydryl Groups and Leukocyte Adherence

Digestive Diseases and Sciences ◽

10.1007/s10620-006-9496-3 ◽

2006 ◽

Vol 52 (1) ◽

pp. 119-125 ◽

Cited By ~ 10

Author(s):

José Maurício S. C. Mota ◽

Pedro M. G. Soares ◽

Álvaro A. J. Menezes ◽

Henrique P. Lemos ◽

Fernando Q. Cunha ◽

...

Keyword(s):

Sulfhydryl Groups ◽

Gastric Damage ◽

Leukocyte Adherence ◽

Protein Sulfhydryl ◽

Protein Sulfhydryl Groups

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Evaluation of the Nutrient Status of Some Hydrophytes in the Water Courses of Nile Delta, Egypt

Journal of Botany ◽

10.1155/2009/862565 ◽

2009 ◽

Vol 2009 ◽

pp. 1-11 ◽

Cited By ~ 4

Author(s):

Kamal H. Shaltout ◽

Tarek M. Galal ◽

Thanaa M. El-Komi

Keyword(s):

Nile Delta ◽

Nutrient Status ◽

The Other ◽

Ceratophyllum Demersum ◽

Chemical Compositions ◽

Net Energy ◽

Ether Extract ◽

Total Carbohydrate ◽

Vegetative Phase ◽

Water Courses

The nutritive values of three dominant hydrophytes along the water courses in Nile Delta, Egypt (Echinochloa stagnina, Eichhornia crassipes, and Ceratophyllum demersum) were evaluated in terms of estimating their phytomass, organic, and inorganic chemical compositions. Shoots were collected seasonally from 25 permanent stands representing the distribution of the three species along 15 canals and 10 drains distributed in 5 localities within the Nile Delta. Living and dead parts and total phytomass were estimated. Their inorganic (Na, K, Ca, Mg, P, Cu, Mn, and Pb) and organic (carbohydrates, total nitrogen, total protein, ether extract, digestible nutrient, digestible energy, metabolized energy, and net energy) contents were estimated. The vegetative phase of E. stagnina extended during winter, spring, and summer, while it is flowering and fruiting during autumn. On the other hand, E. crassipes and C. demersum attained their maximum flowering during spring and maximum fruiting during summer, while maximum vegetative phase during autumn and winter. E. stagnina had the highest mean annual phytomass, while C. demersum had the lowest. The living parts of C. demersum had the highest concentrations of Na, Ca, and Mg, while the living parts of E. crassipes had the highest of K and N. C. demersum had the ability to accumulate more concentrations of heavy metals than the other studied species. E. crassipes had the highest values of total carbohydrate and total proteins, while E. stagnina had the highest of crude fibers, and C. demersum had the highest of ether extract and ash contents. The living parts of E. crassipes and C. demersum were considered as excellent forages, while the dead parts of all species and the living parts of E. stagnina were evaluated as poor forage.

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