Development of technology for producing wheat bread enriched with soy ingredient

Nutritional supplements produced from soy and being rich in protein, fat and many other valuable substances have become a promising source of fortification of bakery products. This study aimed to develop a technology for producing wheat bread enriched with a protein-vitamin-mineral ingredient (PVMI) obtained from germinated soybeans. When kneading the dough, PVMI was added together with wheat flour and other dry components. Mathematical modeling of the wheat bread sensory evaluation showed the optimal content of PVMI in the recipe was 20% of the weight of wheat flour. As a result of the correlation-regression analysis, the most rational production characteristics for obtaining bread enriched with BVMI have been established. The amount of protein in the bread sample increased by 71.9%, dietary fiber - by 40.9%, vitamin E - by 34.7%, vitamin B1 - by 190%, vitamin B2 - by 80%, choline - by 186%. The fat quantity was 4.1 times more. At the same time, the total carbohydrate content decreased by 18.1% compared to the control sample. The improved chemical composition makes it possible to classify wheat bread with PVMI as an enriched food product.

Measurement of macroalgae total carbohydrate content found in Lendang Luar coast, Lombok, Indonesia for potential sources of bioethanol

Bioethanol is a renewable alternative energy through the process of fermenting sugar from carbohydrate sources by adding microorganisms such as yeast or bacteria. Macroalgae or known as seaweed is one of the producers in the marine environment which has a high carbohydrate content so that it can be used as raw material for bioethanol. Macroalgae contains specific carbohydrates including laminarin, mannitol, alginate, agar and polysaccharides. The high carbohydrate content indicates the ethanol content produced. The aim of the study was to initial screen total carbohydrates in red algae (G. latifolium and G rugosa) and brown algae (M rosea, S. crassifolium, S. cristaefolium, S. polycystum, P.australis and T. muray ana). Carbohydrate total analysis was performed by colorimetric assay using the BioVision kit. The overall carbohydrate content found in macroalgae samples in this study was 28.23 fig (DW)1. The highest carbohydrate content was obtained by G. latifolium (37.50 fig (DW)1), followed by G rugosa (34.27 fig (DW)1) and S. cristaefolium (33.33 fig (DW)1). Current results show that macroalgae exhibits sufficient amounts of carbohydrate which could potentially be further developed as source for biotehanol.

Comparison of Nutritional and Anti-Nutritional Qualities of Grewia forbesii Hav. Ex Mast and Grewia bicolor Juss Fruits from Kitapilimwa Forest Reserve in Iringa District

The Grewia forbesii and Grewia bicolor fruits were collected from Kitapilimwa Forest Reserve and were analysed for proximate, nutrients and anti-nutrients compositions. Results showed low moisture contents in G. forbesii and G. bicolor amounting to 14.14 and 13.98 percent, respectively. Crude fibre percent was slightly higher in G. bicolor (33.15%) than G. forbesii (31.95%). Lipid content was slightly higher in G. bicolor (1.37%) compared to G. forbesii (1.28%). The crude protein content of G. forbesii (7.44%) was slightly higher than G. bicolor (7.00%). Total carbohydrate content of G. forbesii (68.9%) was higher than that of G. bicolor (70%). Mineral analysis indicated substantial amounts of potassium 898 mg/100 g in G. forbesii and 879 mg/100 g in G. bicolor. Calcium content was high in G. forbesii and G. bicolor amounting to 453 mg/100 g and 582 mg/100 g, respectively. Iron was high in both species (20.73–24.45 mg/100 g). Manganese, copper and zinc were substantially low. The results revealed low levels of tannin (< 1.7%) and phylates (< 0.3%). The presence of substantial amounts of nutrients and low anti-nutrients revealed that the studied species may be potential sources of nutritional food. Keywords: Proximate analysis, mineral nutrients, anti-nutritional qualities, Grewia species, Kitapilimwa Forest Reserve

Isolation of Prebiotics from Artocarpus integer’s Seed

There has been a high amount of attention given to prebiotics due to their significant physiological function and health benefits. Prebiotics contain nondigestible compounds that allow specific changes, both in the growth and in the activity of bacteria in the host gastrointestinal tract, that provide benefits upon the host by promoting a healthy digestive system and preventing disease. This study aims at investigating the potential prebiotic activity of bioactive compounds extracted from the seeds of an underutilized indigenous plant Artocarpus integer (A. integer). The optimum microwave-assisted extraction conditions were a microwave power of 1500 W, extraction time of 180 s, and solvent-to-sample ratio of 1000 : 1. The maximum amount of the total carbohydrate content extracted from A. integer was 787 mg/L. The percentage hydrolysis levels of A. integer extract in gastric juice at pH 1, 2, 3, and 4 were 6.14%, 7.12%, 8.98%, and 10.23%, respectively. For enzymatic digestion, the percentage of hydrolysis was 0.16% at pH 7. A. integer extract was found to support the growth of probiotics such as L. acidophilus and L. casei. After 72 hours of incubation, L. acidophilus achieved 6.96 log 10 CFU, whereas L. casei reached 8.33 log 10 CFU. The study makes an important contribution to the development of the use of Sarawakian underutilized plants and to the identification of new sources of prebiotic materials to be used in food.

Nutritional, Textural, and Sensory Quality of Aloe Vera Leaf Gel Powder Fortified Plain Cake

Aloe Vera leaves have a great potential as an economic supplement with an adequate nutritional profile. In this study, Aloe Vera leaf gel (AVG) powder was used to fortify plain cakes. Freeze drying of AVG was performed for the production of Aloe Vera powder (ALP) and four plain cakes were prepared with different proportions of ALP for further investigation. Analysis suggested that ALP contained significantly (p&amp;lt;0.05) higher amount of protein (22.23 vs 12.24), ash (19.83 vs 0.64) and iron (175 vs 3.05) content than refined wheat flour (RWF). ALP also contained significant amount of total polyphenols and antioxidant. Moisture, protein, ash, weight, and minerals (Fe, Ca) content were higher (p&amp;lt;0.05) in ALP-cakes; whereas fat, volume, specific volume, height, baking loss, and total carbohydrate content were higher (p&amp;lt;0.05) in RWF-cakes. Incorporation of 6 and 8% ALP in the formulation increased the total polyphenols and anti-oxidant activity in plain cakes. Texture analysis revealed that hardness and chewiness increased in ALP-cakes but decreased in RWF-cakes, however, cohesiveness, springiness, and chewiness decreased in ALP-cakes. Sensory attributes suggested that 4% ALP incorporated cake was attributed as the best formulation. In conclusion, ALP can be supplemented in cakes up to 8% to improve the nutrient value.

Effects of carbohydrate content in seeds on the germination and viability after cryopreservation

This article is devoted to the study of the influence of cryopreservation on the preservation of sowing qualities of seeds of representatives of the genus Picea: P. asperata (Rough spruce) and P. pungens (Spiny spruce). As an alternative shock method of cryopreservation, the method of step-by-step 3-step temperature reduction was used 1 stage +4 °C, 2 stage –18 °C, 3 stage –196 °C liquid nitrogen. Additionally, the effect of 4 time intervals (1 hour, 24 hours, 72 hours and 168 hours) of step freezing on seed quality was investigated. To explain the results by photometry, the total carbohydrate content of the seeds is determined before the 3rd freezing step (cryo interaction). The analysis of the results of germination showed that the seeds of the studied species respond in different directions to the step cryo freezing. So, seeds of P. asperata germination in all tree experimental variants are higher on 4–32 % than in the control variant; and the maximum results are revealed in the variant with time intervals of 168 hours. For P. pungens seeds, the germination rates in experimental variants were on 18–22 % lower than in the control; and the largest germination rate of seeds among experimental variations are revealed in variant with interval 24 hours. The determination of the total carbohydrate content of the tested seeds at different cryogenic freezing intervals found that this indicator varied significantly depending on the time interval. The lowest values of total carbohydrates are found in variation with a time interval — 24 hours. The maximum total carbohydrate content is determined in a variation with a time interval — 1 hour. Correlation analysis between seed germination indices and total carbohydrate content established a high relationship between these indices. So, for P. asperata, the value of the Pearson coefficient was 0.86, and for P. pungens seeds — 0.91.

Differences in water relations and total carbohydrate content between precommercially thinned and unthinned Pinus nigra subsp. pallasiana stands

Variations in seasonal responses in water relations and total carbohydrate content (TCC) in one-yearold shoots from precommercially thinned (PCT) and unthinned Anatolian black pine stands were assessed during three seasons (sampling in May, July and September) in 2015–2017. Three different treatments were established: unthinned control with 4 941 stems·ha–1 and two thinned spacing levels (2–2.5 and 3–3.5 m) where 2 133 stems·ha^–1 and 1 093 stems·ha^–1 were left, respectively. Differences in osmotic potential at turgor loss point (Ψπ_TLP) between the thinned and unthinned plots appeared only during a water shortage (September) in the second season, with the thinned stands showing lower Ψπ_TLP than the unthinned stands. Seasonal variation in terms of Ψπ_TLP was detected in the 3–3.5 m spacing trees. PCT were effective on osmotic potential at full turgor (Ψπ₁₀₀), relative water content (RWC), symplastic water at saturated point per dry weight of the shoot and dry weight fraction. In both the thinned and unthinned plots, a gradual decline was observed in RWC from May to September in all three years. Generally, although TCC was found to be higher in the 3–3.5 m spacing, control plots were also high in carbohydrates in some periods. Results reflect the ability of this species to survive in changing environments by PCT during dry periods in the three studied seasons. Although PCT has an effect on water potential components and TCC, it is not clear exactly how effective the PCT density is in osmotic adjustment.

Optimization of bioethanol production from cassava peels

The bioethanol production from waste is acquiring attraction as a strategy for increasing energy security. This study aims to optimize the production of ethanol from cassava peel using Box Bhenken experimental design. The total carbohydrate content of about 90% in cassava peel was subjected to enzymatic hydrolysis using Alpha-amylase followed by Simultaneous Saccharification and Fermentation (SSF) by Saccharomyces cerevisiae for bioethanol production. The production of bioethanol from cassava peels was investigated for 1-4 hours (hydrolysis time), 0.5–1.5mg/L (enzyme loading), and 1-5 days (incubation time). A statistical model was developed and validated to predict the yield of bioethanol after fermentation, and the Response Surface Methodology (RSM) was used to optimize the conditions. The results revealed that the maximum ethanol yield of 1.911% was obtained at the optimum hydrolysis time, enzyme loading, and incubation time (i.e. 2.5 hours, 1 mg/L, and 3 days respectively).

Could the Content of Soluble Carbohydrates in the Young Shoots of Selected Willow Cultivars Be a Determinant of the Plants’ Attractiveness to Cervids (Cervidae, Mammalia)?

Ten willow cultivars grown in experimental plots were evaluated for performance, attractiveness to foragers, and the content and composition of soluble carbohydrates. The survival of willow cuttings in a thicket and in browse plots differed subject to cultivar, soil quality, and soil moisture content. The number of stump sprouts varied considerably, from 1.1 shoots in the weakest soils in Słonin, Poland, to 3.43 in the plot in Czempin, Poland. Browse plots were established in 2017. They were cut, and fencing was removed in early spring of 2019. Young shoots (10 cm shoot tip with buds, preferably eaten by animals) were sampled for analyses of soluble carbohydrates as potential attractors for foraging cervids. All willow cultivars contained the same soluble carbohydrates: glucose, fructose, galactose, sucrose, myo-inositol, galactinol, and raffinose. Total carbohydrate content ranged from 21.31 (S. amygdalina 1045) to 69.37 mg/g−1 DM (dry matter) (S. purpurea). Glucose was the predominant soluble sugar in the shoots of all willow cultivars, excluding S. viminalis. The fructose content of the shoots was approximately twice lower than their glucose content in all willow cultivars. Smaller differences were observed in the content of myo-inositol, which ranged from 4.61 (S. amygdalina 1045) to 8.26 mg/g−1 DM (S. fragilis cv. Kamon/Resko). The phloem of all willow species contained small quantities of galactinol and trace amounts of raffinose. Weak negative correlations were noted between total carbohydrate content, the content of glucose, fructose, and galactose vs. the attractiveness of willow shoots to foraging cervids. The remaining carbohydrates that occurred in smaller quantities in willow shoots were not correlated with their attractiveness to cervids.

Rice and rice bran from different cultivars: physicochemical, spectroscopic, and thermal analysis characterization

Different rice cultivars have been developed in order to obtain grains with optimal cultivation and compositional characteristics, which affect their potential applications. Therefore, the characterization of these different rice cultivars is required. In the present study, white rice and rice bran from the cultivars BRS AG, BRS Pampa, and BRS 358 provided by EMBRAPA were characterized by physicochemical means, infrared spectroscopy (FTIR-ATR), and thermal analyses. The moisture, lipid, and ash contents did not differ among the white rice cultivars. The cultivar BRS Pampa exhibited the highest protein and lowest total carbohydrate contents. Both BRS Pampa and BRS 358 showed a higher phytic acid content than BRS AG. The highest total carbohydrate content was observed in BRS AG white rice samples, which confirmed its suitability for use in ethanol production. Among the rice bran samples, BRS 358 demonstrated the highest contents of lipid, protein, and phytic acid, and the lowest total carbohydrate content. FTIR-ATR and thermal analyses were suitable for correlating the physicochemical properties of white rice and rice bran with the molecular composition in the respective cultivars studied. Both white rice and rice bran exhibited a thermal degradation temperature at 300 °C. Lipids, protein, ash and phytic acid were considerably higher in rice bran than white rice in all cultivars studied, which demonstrates the importance of the use of this by-product.