scholarly journals Comparative experimental study of 5-ALA and 5-ALA hexyl ester specific activity

2018 ◽  
Vol 7 (3) ◽  
pp. 43-46 ◽  
Author(s):  
R. I. Yakubovskaya ◽  
A. A. Pankratov ◽  
E. V. Filonenko ◽  
E. A. Lukyanets ◽  
V. I. Ivanova-Radkevich ◽  
...  
Keyword(s):  
Experimental Study ◽  
Fluorescence Intensity ◽  
Aminolevulinic Acid ◽  
Specific Activity ◽  
Protoporphyrin Ix ◽  
Bladder Epithelium ◽  
Hexyl Ester ◽  
Rabbit Bladder

A comparative experimental study of the specific activity of drugs based on 5-aminolevulinic acid (5-ALA) and its hexyl ester (5-ALA HE) was carried out. Their ability to induce the synthesis of photoactive protoporphyrin IX in the healthy tissues of the rabbit bladder when instilling the drug solutions at various concentrations has been estimated. It was shown that 5-ALA HE results in the induction and accumulation of PPIX in the rabbit bladder epithelium at much lower concentrations than 5-ALA. Thus, a significant increase in the fluorescence intensity in comparison with the control was achieved by instillation of 5-ALA HE solution in the rabbit’ bladder at a concentration of only 0.0001% (fluorescence intensity 2.20±0.60 a.u.), and for 5-ALA – only when using a solution at a concentration of 0.3% (fluorescence intensity 2.60±1.02 a.u.).

Experimental Study to Understand Nonspecific Protoporphyrin IX Fluorescence in Brain Tissues Near Tumors After 5-Aminolevulinic Acid Administration

2013 ◽  
Vol 31 (9) ◽  
pp. 428-433 ◽  
Author(s):  
Takahiro Masubuchi ◽  
Yoshinaga Kajimoto ◽  
Shinji Kawabata ◽  
Naosuke Nonoguchi ◽  
Takahiro Fujishiro ◽  
...  
Keyword(s):  
Experimental Study ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix ◽  
Acid Administration ◽  
Brain Tissues

scholarly journals Динамика накопления протопорфирина IX, индуцированного 5-аминолевулиновой кислотой в трех клеточных линиях разного происхождения

2021 ◽  
Vol 91 (1) ◽  
pp. 162
Author(s):  
Д.А. Горбенко ◽  
А.В. Белашов ◽  
Т.Н. Беляева ◽  
Е.С. Корнилова ◽  
И.К. Литвинов ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Lung Adenocarcinoma ◽  
Cell Cultures ◽  
Fluorescence Intensity ◽  
Human Lung ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix ◽  
3T3 Cells ◽  
Confocal Fluorescence ◽  
Lung Adenocarcinoma A549

By applying the confocal fluorescence microscopy, we investigated the dynamics of the synthesis and accumulation of protoporphyrin IX induced by 5-aminolevulinic acid (5-ALA) in three cell lines: malignant - human epithelial carcinoma HeLa and human lung adenocarcinoma A549 - and fibroblast-like immortalized mouse embryonic 3T3 cells. The accumulation of protoporphyrin IX in cell cultures was evaluated based on the analysis of its fluorescence intensity. The dynamics of changes in fluorescence intensity was studied depending upon the duration of cells incubation with 5-ALA and its concentration.

Matrix mediated fluorescence enhancement — why nanoparticles and PPIX make a friendly couple

2012 ◽  
Vol 16 (07n08) ◽  
pp. 878-884
Author(s):  
Gesine Heuck ◽  
Norbert Lange
Keyword(s):  
Aminolevulinic Acid ◽  
Polymeric Nanoparticles ◽  
Protoporphyrin Ix ◽  
Direct Interaction ◽  
Neoplastic Tissue ◽  
Systemic Bioavailability ◽  
Bladder Carcinoma Cell Line ◽  
Hexyl Ester ◽  
Particle Size And Morphology ◽  
Size And Morphology

The hexyl ester of 5-aminolevulinic acid (HAL) is an efficient inducer of the endogenous photosensitizer protoporphyrin IX (PPIX) in neoplastic tissue and approved for the fluorescence photodetection of bladder cancer. It is, however, limited to topical application due to unsuitable pharmacokinetic and pharmacodynamic properties following systemic administration. In this study we sought to prepare HAL laden polymeric nanoparticles made from polylactic acid (PLA) as possible means to improve the systemic bioavailability of HAL. Nanoparticles were prepared by nanoprecipitation. Particle size and morphology of freshly prepared and dehydrated nanoparticles were assessed and the ability to generate PPIX fluorescence was tested on a T24 human bladder carcinoma cell line. Fluorescence intensities of cells incubated with nanoencapsulated HAL were significantly higher than in presence of free HAL. Interestingly, the effect was — at least partly — provoked by the direct interaction of exogenously induced PPIX with the nanoparticles.

scholarly journals Quantitative Spectroscopic Analysis of 5-Aminolevulinic Acid-Induced Protoporphyrin IX Fluorescence Intensity in Diffusely Infiltrating Astrocytomas

2007 ◽  
Vol 47 (2) ◽  
pp. 53-57 ◽  
Author(s):  
Ryutaro ISHIHARA ◽  
Yoichi KATAYAMA ◽  
Takao WATANABE ◽  
Atsuo YOSHINO ◽  
Takao FUKUSHIMA ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Spectroscopic Analysis ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix

Protoporphyrin IX Fluorescence Photobleaching and the Response of Rat Barrett's Esophagus Following 5-aminolevulinic Acid Photodynamic Therapy

2006 ◽  
Vol 82 (6) ◽  
pp. 1638 ◽  
Author(s):  
Ingrid A. Boere ◽  
Dominic J. Robinson ◽  
Henriette S. de Bruijn ◽  
Jolanda Kluin ◽  
Hugo W. Tilanus ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Barrett’S Esophagus ◽  
Barrett's Esophagus ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix
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