Length measurements in digital images by using an anchored discrete convolution algorithm

The ribonucleic acid (RNA) of paramyxoviruses has been characterized by biochemical and physiochemical methods. However, paramyxovirus RNA molecules have not been studied by electron microscopy. The molecular weights of these single-stranded viral RNA molecules are not known as yet. Since electron microscopy has been found to be useful for the characterization of single-stranded RNA, this investigation was initiated to examine the morphology and length measurements of paramyxovirus RNA's.Sendai virus Z strain and Newcastle disease virus (NDV), Milano strain, were used. For these studies it was necessary to develop a method of extracting RNA molecules from purified virus particles. Highly purified Sendai virus was treated with pronase (300 μg/ml) at 37°C for 30 minutes and the RNA extracted by the sodium dodecyl sulfate (SDS)-phenol procedure.

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Nucleic Acid Molecules Prepared by Monolayer Techniques

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094140 ◽

1972 ◽

Vol 30 ◽

pp. 178-179

Author(s):

Dimitrij Lang

Keyword(s):

Electron Microscopy ◽

Standard Deviation ◽

Nucleic Acid ◽

Cytochrome C ◽

Nucleic Acids ◽

Contour Length ◽

Sample Standard Deviation ◽

Molecular Weights ◽

Length Measurements ◽

Protein Monolayer

The success of the protein monolayer technique for electron microscopy of individual DNA molecules is based on the prevention of aggregation and orientation of the molecules during drying on specimen grids. DNA adsorbs first to a surface-denatured, insoluble cytochrome c monolayer which is then transferred to grids, without major distortion, by touching. Fig. 1 shows three basic procedures which, modified or not, permit the study of various important properties of nucleic acids, either in concert with other methods or exclusively:1) Molecular weights relative to DNA standards as well as number distributions of molecular weights can be obtained from contour length measurements with a sample standard deviation between 1 and 4%.

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Reduction in size of digital images: does it lead to less detectability or loss of diagnostic information?

Dentomaxillofacial Radiology ◽

10.1038/sj.dmfr.4600329 ◽

1998 ◽

Vol 27 (2) ◽

pp. 93-96 ◽

Cited By ~ 12

Author(s):

C H Versteeg ◽

G C H Sanderink ◽

S R Lobach ◽

P F van der Stelt

Keyword(s):

Digital Images ◽

Diagnostic Information

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Development of a system for craniofacial analysis from monitor-displayed digital images

Dentomaxillofacial Radiology ◽

10.1038/sj.dmfr.4600420 ◽

1999 ◽

Vol 28 (2) ◽

pp. 123-126 ◽

Cited By ~ 28

Author(s):

E Gotfredsen ◽

J Kragskov ◽

A Wenzel

Keyword(s):

Digital Images

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Sonographic longitudinal cervical length measurements in nulliparous women at term; prediction of spontaneous onset of labour

Zeitschrift für Geburtshilfe und Neonatologie ◽

10.1055/s-2007-1002958 ◽

2007 ◽

Vol 211 (S 2) ◽

Author(s):

M Meijer-Hoogeveen ◽

C Van Holsbeke ◽

I van der Tweel ◽

P Stoutenbeek ◽

GHA Visser

Keyword(s):

Cervical Length ◽

Term Prediction ◽

Nulliparous Women ◽

Length Measurements ◽

Spontaneous Onset

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