Research on gene expression mechanism of mechanical products based on the behavior surface

Various algorithms have been devised to mathematically model the dynamic mechanism of the gene expression data. Gillespie’s stochastic simulation (GSSA) has been exceptionally primal for chemical reaction synthesis with future ameliorations. Several other mathematical techniques such as differential equations, thermodynamic models and Boolean models have been implemented to optimally and effectively represent the gene functioning. We present a novel mathematical framework of gene expression, undertaking the mathematical modeling of the transcription and translation phases, which is a detour from conventional modeling approaches. These subprocesses are inherent to every gene expression, which is implicitly an experimental outcome. As we foresee, there can be modeled a generality about some basal translation or transcription values that correspond to a particular assay.

Novel gene expression mechanism in a fission yeast retroelement: Tf1 proteins are derived from a single primary translation product.

The EMBO Journal ◽

10.1002/j.1460-2075.1993.tb06178.x ◽

1993 ◽

Vol 12 (12) ◽

pp. 4885-4895 ◽

Cited By ~ 41

Author(s):

H.L. Levin ◽

D.C. Weaver ◽

J.D. Boeke

Keyword(s):

Gene Expression ◽

Fission Yeast ◽

Translation Product ◽

Novel Gene ◽

Primary Translation Product ◽

Expression Mechanism

Tat has a dual role in simian immunodeficiency virus transcription

Journal of General Virology ◽

10.1099/vir.0.044511-0 ◽

2012 ◽

Vol 93 (10) ◽

pp. 2279-2289 ◽

Cited By ~ 6

Author(s):

Gisela J. van der Velden ◽

Monique A. Vink ◽

Ben Berkhout ◽

Atze T. Das

Keyword(s):

Gene Expression ◽

Simian Immunodeficiency Virus ◽

Cultured Cells ◽

Virus Transcription ◽

Immunodeficiency Virus ◽

Dna Elements ◽

Tar Rna ◽

Essential Function ◽

Expression Mechanism

Tat has a pivotal role in human and simian immunodeficiency virus (HIV and SIV) replication because it stimulates transcription by binding to the trans-activator response (TAR) element. In addition, several other Tat functions have been proposed. Most studies have focused on HIV-1 Tat and much less is known about SIV Tat. An SIVmac239 variant was constructed previously in which the Tat–TAR transcription mechanism is functionally replaced by the doxycycline-inducible Tet-On gene expression mechanism (SIV-rtTA). In this study, SIV-rtTA variants were used to analyse the functions of SIV Tat. It was shown that Tat-minus SIV-rtTA variants replicated efficiently in PM1 T-cells, ruling out an additional essential Tat function. Nevertheless, replication was suboptimal in other cells, and evolutionary pressure to repair Tat expression was documented. It was demonstrated that SIV-rtTA required Tat for optimal gene expression, despite the absence of the Tat–TAR axis. This Tat effect was lost upon replacement of the long terminal repeat promoter region by a non-related promoter. These results indicate that Tat can activate SIV transcription via TAR RNA and U3 DNA elements but has no other essential function in replication in cultured cells. The experiments were limited to cell lines and PBMCs, and did not exclude an accessory Tat function under specific conditions or in vivo.

Complementation hypothesis: the necessity of a monoallelic gene expression mechanism in mammalian development

Cytogenetic and Genome Research ◽

10.1159/000090811 ◽

2006 ◽

Vol 113 (1-4) ◽

pp. 24-30 ◽

Cited By ~ 16

Author(s):

T. Kaneko-Ishino ◽

T. Kohda ◽

R. Ono ◽

F. Ishino

Keyword(s):

Gene Expression ◽

Mammalian Development ◽

Expression Mechanism

Regulation of interferon gene expression: mechanism of action of the If-1 locus.

Journal of Virology ◽

10.1128/jvi.62.4.1125-1131.1988 ◽

1988 ◽

Vol 62 (4) ◽

pp. 1125-1131 ◽

Cited By ~ 4

Author(s):

L Daigneault ◽

B Coulombe ◽

D Skup

Keyword(s):

Gene Expression ◽

Mechanism Of Action ◽

Interferon Gene ◽

Expression Mechanism

Calcium-mediated Gene Expression: Mechanism for Neuronal Plasticity and Survival

The Neuroscientist ◽

10.1177/107385849500100603 ◽

1995 ◽

Vol 1 (6) ◽

pp. 317-320 ◽

Cited By ~ 4

Author(s):

Steven Finkbeiner

Keyword(s):

Gene Expression ◽

Neuronal Plasticity ◽

Expression Mechanism

Alginate-Dependent Gene Expression Mechanism in Sphingomonas sp. Strain A1

Journal of Bacteriology ◽

10.1128/jb.01666-14 ◽

2014 ◽

Vol 196 (14) ◽

pp. 2691-2700 ◽

Cited By ~ 11

Author(s):

C. Hayashi ◽

R. Takase ◽

K. Momma ◽

Y. Maruyama ◽

K. Murata ◽

...

Keyword(s):

Gene Expression ◽

Expression Mechanism

Minor introns are embedded molecular switches regulated by highly unstable U6atac snRNA

eLife ◽

10.7554/elife.00780 ◽

2013 ◽

Vol 2 ◽

Cited By ~ 42

Author(s):

Ihab Younis ◽

Kimberly Dittmar ◽

Wei Wang ◽

Shawn W Foley ◽

Michael G Berg ◽

...

Keyword(s):

Gene Expression ◽

Tumor Suppressor ◽

Mrna Expression ◽

Cytokine Production ◽

Molecular Switches ◽

Evolutionary Conservation ◽

Cell Stress ◽

Rna Polymerases ◽

High Conservation ◽

Expression Mechanism

Eukaryotes have two types of spliceosomes, comprised of either major (U1, U2, U4, U5, U6) or minor (U11, U12, U4atac, U6atac; <1%) snRNPs. The high conservation of minor introns, typically one amidst many major introns in several hundred genes, despite their poor splicing, has been a long-standing enigma. Here, we discovered that the low abundance minor spliceosome’s catalytic snRNP, U6atac, is strikingly unstable (t½<2 hr). We show that U6atac level depends on both RNA polymerases II and III and can be rapidly increased by cell stress-activated kinase p38MAPK, which stabilizes it, enhancing mRNA expression of hundreds of minor intron-containing genes that are otherwise suppressed by limiting U6atac. Furthermore, p38MAPK-dependent U6atac modulation can control minor intron-containing tumor suppressor PTEN expression and cytokine production. We propose that minor introns are embedded molecular switches regulated by U6atac abundance, providing a novel post-transcriptional gene expression mechanism and a rationale for the minor spliceosome’s evolutionary conservation.