Molecular Detection of Human Herpes Virus-8 in Prostatic Adenocarcinoma and Benign Prostatic Hyperplasia Tissues by DNA -In Situ Hybridization

Human Herpes Virus-8 (HHV-8) is a sexually transmitted viral infection that can infect the prostate epithelium in immunocompromised adults. Recently, HHV-8 was related to the development and progression of several human malignancies like prostatic adenocarcinoma. This retrospective research was designed to analyze the distribution and possible impact of HHV-8 infection on prostatic adenocarcinogenesis. A total number of one hundred formalin-fixed prostatic tissues were enrolled in this research; forty Prostate Adenocarcinoma (PAC) biopsies, forty biopsies from Benign Prostatic Hyperplasia (BPH), and twenty Apparently Normal Prostatic Tissues (ANPT) as a control group. Detection of HHV -8 DNA was achieved by a highly-sensitive variant of Chromogenic In Situ Hybridization (CISH) technique. In the mean age of PAC patients was 64.08±7.54years. Detection of CISH reactions for HHV 8- DNA was observed in tissues of 70% (28 out of 40) of PAC patients and 30% (12 out of 40) of BPH tissues, whereas no positive reactions were detected in the ANPT group. Detection of CISH reactions for HHV 8- DNA was observed in 55.6% of tissues of PAC patients with well grade histopathological examination, 87.5% of moderate grade, and 69.6% of poorly differentiated grade. It can be concluded that HHV-8 infection might contribute in prostate oncogenesis, together with other essential oncogenic viruses.

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DNA-In Situ Localization of Human Herpes Virus-8 and Mutated P57 Genes Sequences in Tissues from a Group of Female Iraqi Patients with Breast Tumors

Indian Journal of Public Health Research & Development ◽

10.5958/0976-5506.2018.01959.9 ◽

2018 ◽

Vol 9 (12) ◽

pp. 879

Author(s):

Israa Habeeb Nasser ◽

Shakir H. Mohammed Al-Alwany

Keyword(s):

Herpes Virus ◽

Breast Tumors ◽

Human Herpes ◽

Human Herpes Virus 8 ◽

Human Herpes Virus ◽

Herpès Virus

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In situ Molecular Hybridization of Kaposi, s Sarcoma Associated Virus (Human Herpes Virus 8) in Nasopharyngeal Carcinoma Tissues

Indian Journal of Forensic Medicine & Toxicology ◽

10.37506/ijfmt.v15i1.13682 ◽

2021 ◽

Keyword(s):

Nasopharyngeal Carcinoma ◽

Herpes Virus ◽

Molecular Hybridization ◽

Human Herpes ◽

Human Herpes Virus 8 ◽

Human Herpes Virus ◽

Herpès Virus

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Detection of human herpes virus 6 in AIDS-associated retinitis by means of in situ hybridization, polymerase chain reaction and immunohistochemistry

Journal of Medical Virology ◽

10.1002/(sici)1096-9071(199608)49:4<289::aid-jmv6>3.0.co;2-# ◽

1996 ◽

Vol 49 (4) ◽

pp. 289-295 ◽

Cited By ~ 26

Author(s):

Anne-Marie Fillet ◽

Isabelle Reux ◽

Cathy Joberty ◽

Jean-Guy Fournier ◽

Jean-Jacques Hauw ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

In Situ Hybridization ◽

Herpes Virus ◽

Human Herpes ◽

Chain Reaction ◽

Human Herpes Virus ◽

Human Herpes Virus 6 ◽

Polymerase Chain ◽

Herpès Virus

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Chromogenic in Situ Hybridization for Human Cytomegalovirus-DNA Detection in Tissue Subsets with Prostatic Adenocarcinoma and Benign Hyperplasia

Iraqi Journal of Science ◽

10.24996/ijs.2021.62.9.6 ◽

2021 ◽

pp. 2894-2905

Author(s):

Tayseer Anmar Hassan ◽

Jenan M. Jawad AL-Saffar ◽

Saad Hasan Mohammed Ali

Keyword(s):

In Situ Hybridization ◽

Benign Prostatic Hyperplasia ◽

Human Cytomegalovirus ◽

Dna Detection ◽

Prostatic Hyperplasia ◽

Prostatic Adenocarcinoma ◽

Wide Range ◽

Chromogenic In Situ Hybridization ◽

Benign Hyperplasia

Human cytomegalovirus (HCMV) infects a wide range of human cells, resulting in both benign and malignant tumors. In the last few decades, proteins and/or nucleic acids of the virus were found to be often highly expressed in in patients with basal cell hyperplasia and prostatic neoplasia. This research aimed to unravel the rate of HCMV infections among prostatic tissue subsets from Iraqi patients with adenocarcinoma and benign hyperplasia. One hundred, formalin-fixed and paraffin embedded prostatic tissues were obtained from 40 tissue samples collected from different grades of prostate carcinoma; 40 from benign prostatic hyperplasia and 20 from apparently healthy prostatic tissues. These tissue specimens were collected from the archives of different public and private histopathological laboratories in Baghdad. Detection of HCMV-DNA was achieved by a highly sensitive version of chromogenic in situ hybridization technique. The signals of chromogenic in situ hybridization reactions for HCMV-DNA detection in prostatic adenocarcinoma tissues were found in 65% (26 out of 40) of the tissues, whereas in BPH (Benign Prostatic Hyperplasia), HCMV-DNA was detected in 57.5% (23 out of 40) of the tissues, and in the healthy control group in 25% (5 out of 20) of the tissues. The highest percentage of positive- HCMV- DNA-CISH reactions (57.5%) was found in prostatic adenocarcinomatous tissues that showed poor differentiation. Our results could show that HCMV might contribute to the development of the studied subsets of prostatic adenocarcinoma and benign prostatic hyperplasia.

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