Restoration of Male Fertility in Seasonally Dependent Male Sterile Mutant Tomato, Lycopersicon esculentum cv. First.

Abstract Maize male sterile mutant 40 (ms40) was obtained from the progeny of ethyl methanesulfonate (EMS) treated inbred line RP125. Genetic analysis showed that it was controlled by a single recessive nuclear gene. Cytological observation of anthers revealed that abnormal cuticles and disappearing of Ubisch bodies presented in ms40. Moreover, its tapetum exhibited delayed degradation and blocked the formation of abnormal microspore. Using map-based cloning, ms40 locus was located in a 282-kb interval on chromosome 4, five annotated genes were predicted within this region. PCR-based sequencing detected a single nonsynonymous SNP (G>A) which changed glycine (G) to arginine (A) in the seventh exon of Zm00001d053895, while no difference was found for the other four genes between ms40 and RP125. Zm00001d053895 encodes the bHLH transcription factor bHLH51 which protein was located at nuclear. Phylogenetic analysis presented that bHLH51 had the highest homology with Sb04g001650, a tapetum degeneration retardation (TDR) bHLH transcription factor in Sorghum bicolor. Co-expression analysis exposed a total of 1192 genes coexpressed with Zm00001d053895 in maize, 647 out of 1192 were anther-specific genes. In summary, these findings are conducive to the marker-assisted selection of ms40 in hybrid breeding and laid a foundation for further studies on the mechanisms of male fertility.

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Tasselyzer, a machine learning method to quantify anther extrusion in maize, based on PlantCV

10.1101/2021.09.27.461799 ◽

2021 ◽

Author(s):

Chong Teng ◽

Noah Fahlgren ◽

Blake C. Meyers

Keyword(s):

Male Fertility ◽

Large Scale ◽

Smart Phone ◽

Temperature Sensitive ◽

Male Sterile ◽

Automated Method ◽

Anther Extrusion ◽

Quantitative Image ◽

Sterile Mutant

Male fertility in maize is controlled by development and genetic programming and is directly impacted by environmental factors such as light, temperature, water, and nutrient availability; the control of this trait has substantial agronomic utility. Maize anthers emerge from male florets, which are clustered to form the tassel at the top of the plant separated from the female ear. Quantification of anther extrusion is one important aspect in the determination of male fertility. To address the lack of an automated method to measure anther extrusion on a large scale, we developed 'Tasselyzer', a quantitative, image-based color trait analysis pipeline for tassel image segmentation, based on the existing PlantCV platform, and we applied it to determine the proportion of anther extrusion. We evaluated Tasselyzer in maize during the seven-day period of pollen shedding as well as in the temperature-sensitive male sterile mutant dcl5. With tassel images obtained with a smart phone camera, we show that the anther scores positively correlate with anther extrusion, and such methods can be used to measure environmental impacts on the dcl5 mutant. Altogether, this work establishes an automated and inexpensive method to quantify anther extrusion in maize, which would be useful for research and breeding.

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Genetic Analysis and Molecular Mapping of a Male Sterile Mutant in Rice

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2009.01151 ◽

2009 ◽

Vol 35 (6) ◽

pp. 1151-1155

Author(s):

Ming-Guang CHU ◽

Shuang-Cheng LI ◽

Shi-Quan WANG ◽

Qi-Ming DENG ◽

Jing ZHANG ◽

...

Keyword(s):

Genetic Analysis ◽

Molecular Mapping ◽

Male Sterile ◽

Sterile Mutant

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The Dynamics of Gynodioecy in Plantago lanceolatu L. II. Mode of Action and Frequencies of Restorer Alleles

Genetics ◽

10.1093/genetics/147.3.1317 ◽

1997 ◽

Vol 147 (3) ◽

pp. 1317-1328

Author(s):

Anita A de Haan ◽

Hans P Koelewijn ◽

Maria P J Hundscheid ◽

Jos M M Van Damme

Keyword(s):

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Allele Frequency ◽

Mode Of Action ◽

Male Fertility ◽

Fertility Restoration ◽

Plantago Lanceolata ◽

Allele Frequencies ◽

Male Sterile ◽

Male Fertility Restoration

Male fertility in Plantago lanceolata is controlled by the interaction of cytoplasmic and nuclear genes. Different cytoplasmic male sterility (CMS) types can be either male sterile or hermaphrodite, depending on the presence of nuclear restorer alleles. In three CMS types of P. lanceolata (CMSI, CMSIIa, and CMSIIb) the number of loci involved in male fertility restoration was determined. In each CMS type, male fertility was restored by multiple genes with either dominant or recessive action and capable either of restoring male fertility independently or in interaction with each other (epistasis). Restorer allele frequencies for CMSI, CMSIIa and CMSIIb were determined by crossing hermaphrodites with “standard” male steriles. Segregation of male steriles vs. non-male steriles was used to estimate overall restorer allele frequency. The frequency of restorer alleles was different for the CMS types: restorer alleles for CMSI were less frequent than for CMSIIa and CMSIIb. On the basis of the frequencies of male steriles and the CMS types an “expected” restorer allele frequency could be calculated. The correlation between estimated and expected restorer allele frequency was significant.

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TMT-based comparative proteomic analysis of the male-sterile mutant ms01 sheds light on sporopollenin production and pollen development in wucai (Brassica campestris L.)

Journal of Proteomics ◽

10.1016/j.jprot.2021.104475 ◽

2022 ◽

pp. 104475

Author(s):

Xiaoyan Tang ◽

Mingming Liu ◽

Guohu Chen ◽

Lingyun Yuan ◽

Jinfeng Hou ◽

...

Keyword(s):

Proteomic Analysis ◽

Pollen Development ◽

Brassica Campestris ◽

Male Sterile ◽

Comparative Proteomic Analysis ◽

Sterile Mutant

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OsMYB80 Regulates Anther Development and Pollen Fertility by Targeting Multiple Biological Pathways

Plant and Cell Physiology ◽

10.1093/pcp/pcaa025 ◽

2020 ◽

Vol 61 (5) ◽

pp. 988-1004 ◽

Cited By ~ 3

Author(s):

Xiaoying Pan ◽

Wei Yan ◽

Zhenyi Chang ◽

Yingchao Xu ◽

Ming Luo ◽

...

Keyword(s):

Pollen Development ◽

Male Fertility ◽

Gene Networks ◽

Affinity Purification ◽

Anther Development ◽

Rna Seq ◽

Male Sterile ◽

New Information ◽

Gel Shift Assay

Abstract Pollen development is critical to the reproductive success of flowering plants, but how it is regulated is not well understood. Here, we isolated two allelic male-sterile mutants of OsMYB80 and investigated how OsMYB80 regulates male fertility in rice. OsMYB80 was barely expressed in tissues other than anthers, where it initiated the expression during meiosis, reached the peak at the tetrad-releasing stage and then quickly declined afterward. The osmyb80 mutants exhibited premature tapetum cell death, lack of Ubisch bodies, no exine and microspore degeneration. To understand how OsMYB80 regulates anther development, RNA-seq analysis was conducted to identify genes differentially regulated by OsMYB80 in rice anthers. In addition, DNA affinity purification sequencing (DAP-seq) analysis was performed to identify DNA fragments interacting with OsMYB80 in vitro. Overlap of the genes identified by RNA-seq and DAP-seq revealed 188 genes that were differentially regulated by OsMYB80 and also carried an OsMYB80-interacting DNA element in the promoter. Ten of these promoter elements were randomly selected for gel shift assay and yeast one-hybrid assay, and all showed OsMYB80 binding. The 10 promoters also showed OsMYB80-dependent induction when co-expressed in rice protoplast. Functional annotation of the 188 genes suggested that OsMYB80 regulates male fertility by directly targeting multiple biological processes. The identification of these genes significantly enriched the gene networks governing anther development and provided much new information for the understanding of pollen development and male fertility.

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