scholarly journals Gambaran Jumlah Eosinofil Darah Tepi Penderita Asma Bronkial di Bangsal Paru RSUP Dr. M. Djamil Padang

2013 ◽  
Vol 2 (3) ◽  
pp. 122
Author(s):  
Demas Nico M Manurung ◽  
Ellyza Nasrul ◽  
Irvan Medison
Keyword(s):  
Bronchial Asthma ◽  
Peripheral Blood ◽  
Basic Protein ◽  
Eosinophil Cationic Protein ◽  
Asthma Patient ◽  
Blood Eosinophil ◽  
Major Basic Protein ◽  
Cationic Protein ◽  
Peripheral Blood Eosinophil ◽  
Bronchial Asthma Patient

AbstrakAsma merupakan penyakit gangguan inflamasi kronis saluran pernapasan dengan banyak sel serta elemen seluler yang berperan. Asma dihubungkan dengan hiperresponsif bronkus, keterbatasan aliran udara dan gejala pernapasan yang bersifat reversibel. Salah satu sel yang diketahui berperan besar dalam patogenesis asma adalah eosinofil. Eosinofil melepaskan berbagai mediator seperti Major Basic Protein, Eosinophil Cationic Protein, peroksidase eosinofil, leukotrien C4, serta Platelet-Activating Faktor yang akan merusak epitel saluran napas serta menyebabkan peradangan. Penelitian ini dilaksanakan pada bulan Januari sampai Desember 2012 di Bagian Rekam Medik RSUP Dr. M. Djamil Padang dengan tujuan untuk melihat gambaran jumlah eosinofil darah tepi pada penderita asma bronkial yang dirawat inap. Penelitian yang dilakukan bersifat deskriptif dengan tinjauan retrospektif. Penelitian dilakukan dengan melihat data rekam medis penderita asma bronkial yang dirawat inap di Bangsal Paru RSUP Dr. M. Djamil Padang periode Januari 2010-Desember 2012. Dalam periode dua tahun tersebut, diperoleh 18 sampel yang digunakan pada penelitian ini. Hasil yang diperoleh ialah 4 penderita memiliki jumlah eosinofil darah tepi normal (40-400/mm3) (22,22%) dan 14 penderita memiliki jumlah eosinofil darah tepi dibawah normal (<40/mm3) (77,78%). Tidak ditemukan penderita asma dengan eosinofilia (>400/mm3). Jumlah eosinofil darah tepi pada penderita asma bronkial lebih banyak dibawah normal.Kata kunci: Asma Bronkial, EosinofilAbstractAsthma is a chronic inflammatory disorder of the airways in which many cells and cellular elements play a role. Asthma is associated with hyperresponsif bronchi, airflow limitation, and respiratory symptomps that are reversible. One of cells which is known to play a major role in asthma pathogenesis is eosinophil. Eosinophil release a variety of mediators such as Major Basic Protein, Eosinophil Cationic Protein, Eosinophil Peroxidase, Leukotriene C4, and Platelet Activating Factor that will damage airway epithelium and cause inflammation. The research was conducted in January 2012 through December 2012 at Medical Records Department of RSUP Dr. M. Djamil Padang aiming to see the overview of peripheral blood eosinophil number in patient with bronchial asthma who are hospitalized. The research conducted is using descriptive retrospective review. The study is done by looking medical record data of bronchial asthma patient hospitalized in pulmonary ward of RSUP Dr. M. Djamil Padang in the period of January 2010-December 2012. In the two year period, there are 18 patients which are used in the study. The results are 4 patients had normal peripheral blood eosinophil number (40-400/mm3)(22,22%), 14 patients had low peripheral blood eosinophil number (<40/mm3)(77,78%). There is no patient with eosinophilia (>400/mm3). Peripheral blood eosinophil number in bronchial asthma patient is mostly low.Keywords: Bronchial Asthma, Eosinophil

scholarly journals Peptides of major basic protein and eosinophil cationic protein activate human mast cells

2020 ◽  
Vol 21 ◽  
pp. 100719 ◽  
Author(s):  
Hiroyuki Ogasawara ◽  
Masahiro Furuno ◽  
Koji Edamura ◽  
Masato Noguchi
Keyword(s):  
Mast Cells ◽  
Basic Protein ◽  
Eosinophil Cationic Protein ◽  
Major Basic Protein ◽  
Cationic Protein ◽  
Human Mast Cells

scholarly journals Serum apelin-12 in bronchial asthma

2014 ◽  
Vol 95 (5) ◽  
pp. 631-636
Author(s):  
V N Mineev ◽  
T M Lalaeva ◽  
A A Kuzmina
Keyword(s):  
Serum Level ◽  
Bronchial Asthma ◽  
Peripheral Blood ◽  
Allergic Sensitization ◽  
Serum Levels ◽  
Atopic Asthma ◽  
Bronchial Obstruction ◽  
Blood Eosinophil ◽  
Inverse Association ◽  
Peripheral Blood Eosinophil

Aim. To evaluate the serum level of apelin-12 in patients with bronchial asthma. Methods. 11 healthy controls and 29 patients with bronchial asthma were examined (among them 19 with atopic asthma and 10 with non-atopic asthma). Serum apelin-12 levels were measured by enzyme immunoassay (ELISA, MyBioSource, USA). SPSS (Statistical Package for the Social Science, V. 13) for Windows was used for statistical analysis. Results. The phenomenon of low apelin-12 serum levels was revealed in patients with bronchial asthma. The most significant decrease of apelin-12 levels was noticed in atopic bronchial asthma. The apelin-12 levels were significantly correlated with a number of indices of bronchial obstruction. Apelin-12 levels correlated with a number of indicators characterizing the grade of bronchial obstruction. Moreover, low apelin-12 levels were suggesting bronchial obstruction. Besides, significant reverse correlation of apelin-12 levels and allergic sensitization was revealed. In addition, statistically significant relation between apelin-12 serum level and peripheral blood eosinophil and lymphocyte counts was revealed. There was an inverse association between apelin-12 serum level and peripheral blood neutrophil count. Conclusion. Literature analysis and our own results allow us to suppose the possible protective role of apelin-12 in bronchial asthma flare. Further studies of apelin-12 and of other isoforms of adipokines are needed for better understanding of the disease.

scholarly journals Decreased expression of eosinophil peroxidase and major basic protein messenger RNAs during eosinophil maturation

Blood ◽  
1992 ◽  
Vol 79 (10) ◽  
pp. 2592-2597 ◽  
Author(s):  
V Gruart ◽  
MJ Truong ◽  
J Plumas ◽  
M Zandecki ◽  
JP Kusnierz ◽  
...  
Keyword(s):  
Time Course ◽  
Basic Protein ◽  
Blot Hybridization ◽  
Eosinophil Cationic Protein ◽  
Northern Blot Hybridization ◽  
Messenger Rnas ◽  
Major Basic Protein ◽  
Regulation Of Expression ◽  
Cationic Protein ◽  
Eosinophil Peroxidase

Abstract We evaluated the levels of mRNAs encoding cationic proteins in peripheral blood eosinophils (PBE) purified from patients with eosinophilia and in eosinophils differentiated from cord blood cells (CBC) by culture with recombinant human interleukin-3 (rhIL-3), rhGM- CSF, and rhIL-5. Messenger RNAs encoding eosinophil peroxidase (EPO), major basic protein (MBP), eosinophil-derived neurotoxin (EDN), and eosinophil cationic protein (ECP) were detected by Northern blot hybridization with the respective specific oligonucleotide probes. In mature PBE, MBP mRNA appeared to be absent, whereas EPO mRNA was barely detectable in only 5 of the 19 patients. In contrast, EDN and ECP mRNAs were observed in the PBE of all patients. In CE, EPO, and MBP, mRNAs were abundant in immature eosinophils and their amounts decreased after differentiation toward eosinophils. ECP and EDN mRNAs followed the same patterns, but mRNAs were less abundant at all timepoints studied. Study of mRNA t1/2 during the time course of differentiation indicated that changes in the stability of the different mRNAs were not responsible for the variations observed in the steady-state levels. Together, these results suggest that regulation of expression differs among EPO, MBP, EDN, and ECP mRNAs during the time course of eosinophil differentiation.

scholarly journals Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique.

1986 ◽  
Vol 34 (11) ◽  
pp. 1399-1403 ◽  
Author(s):  
A Egesten ◽  
J Alumets ◽  
C von Mecklenburg ◽  
M Palmegren ◽  
I Olsson
Keyword(s):  
Basic Protein ◽  
Intracellular Localization ◽  
Protein A ◽  
Eosinophil Cationic Protein ◽  
Specific Marker ◽  
Microscopic Technique ◽  
Major Basic Protein ◽  
Cationic Protein ◽  
Eosinophil Peroxidase ◽  
The Matrix

An immunoelectron microscopic technique using protein A-gold as a specific marker was used for precise intracellular localization of eosinophil granule proteins. Eosinophils from healthy individuals were isolated in metrizamide gradients. Eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) were clearly located in the matrix of the large crystalloid-containing granules. In addition, ECP was probably present in the small granules of eosinophils. Major basic protein (MBP) was present in the crystalloid structure of specific granules. This method can be applied in studies of eosinophil degranulation to trace the release of biological effector molecules.

Sign in / Sign up

Export Citation Format

Share Document