scholarly journals Perubahan Parameter Biokimia, Histopatologi Ginjal Tikus Spraque Dawley Pascahipoksia Oleh Ekstrak Akar Acalypha indica dan Herba Centella asiatica

2019 ◽  
Vol 5 (3) ◽  
pp. 160
Author(s):  
Nurfitri Nurfitri ◽  
Erni Hernawati Purwaningsih ◽  
Vivian Soetikno ◽  
Adisti Dwijayanti ◽  
Novi Silvia Hardiany
Keyword(s):  
Reactive Oxygen Species ◽  
Vitamin C ◽  
Reactive Oxygen ◽  
Centella Asiatica ◽  
Oxygen Species ◽  
Acalypha Indica

Hipoksia kronik merupakan salah satu penyebab penyakit ginjal akibat peningkatan pembentukan Reactive Oxygen Species (ROS)  dalam sel. Kombinasi ekstrak akar Acalypha indica 250 mg/KgBB (AI250) dan Centella asiatica 150 mg/kgBB (CA150) memiliki efek neuroterapi pada tikus Spraque Dawley pascahipoksia. Penelitian dilakukan untuk membuktikan manfaat kombinasi ekstrak etanol dan/atau ekstrak tunggalnya dapat memperbaiki kerusakan ginjal tikus pascahipoksia melalui mekanisme antioksidan. 28 tikus jantan dikelompokkan dalam 7 kelompok: kontrol normal; kontrol hipoksia+air; hipoksia+(AI200+CA150); hipoksia+(AI250+CA100); hipoksia+AI250; hipoksia+CA150; hipoksia+vitamin C. Hipoksia selama 7 hari dalam hypoxic chamber berisi O2 10% dan N2 90%, 1 atm. Setiap kelompok diberi perlakuan selama 7 hari. Pada akhir studi hewan diterminasi. Darah dan organ ginjal diambil untuk pemeriksaan biokimia dan histopatologi.Kombinasi  (AI250+CA100) menurunkan kadar MDA ginjal dan plasma secara bermakna dibandingkan kontrol hipoksia (p=0,001 dan p=0,021) dan AI250 (p=0,003 dan 0,043). Kombinasi AI250+CA100 terjadi penurunan ekspresi relatif mRNA HIF-1α (p=0,014), kadar urea plasma (p=0,001) dan perbaikan lesi intra-glomerulus p=0,013.Kesimpulan: Kombinasi (AI250+CA100) dan tunggal AI250 memiliki aktivitas antioksidan terbaik dalam mencegah kerusakan ginjal pascahipoksia, secara biokimiawi dan histopatologinya.

scholarly journals In vitro regulation of reactive oxygen species formation in red blood cells of homozygous sickle cell patients using Vitamin C

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1212
Author(s):  
Ogechukwu Egini ◽  
Edouard Guillaume ◽  
Titilope Adeyemo ◽  
Chiemeziem Nwanyanwu ◽  
Fnu Shweta ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Vitamin C ◽  
Sickle Cell ◽  
Membrane Damage ◽  
Reactive Oxygen ◽  
Test Group ◽  
Red Cells ◽  
University Teaching ◽  
Oxygen Species ◽  
Ros Formation

Background: Sickle cell patients produce more reactive oxygen species (ROS) than healthy individuals, leading to increased cell membrane damage. Theoretically, reducing ROS formation would preserve red cell membranes of sickle cell patients. Vitamin C is a powerful anti-oxidant capable of inhibiting ROS formation in a variety of situations, by functioning as an electron donor to reduce molecular oxygen. This study aimed to determine whether Vitamin C reduced ROS formation in sickle red cells.Methods:27 homozygous (HbSS) patients were recruited from the outpatient clinics of Lagos University Teaching Hospital, Nigeria, and annex at the Sickle Cell Foundation, Lagos, Nigeria. Demographic information and EDTA patient blood samples were collected. The test group were red cells preincubated in 80uM and 100uM Vitamin C concentrations before stressing with tertbutylhydroperoxide. These were compared to stressed matched controls preincubated in phosphate buffered saline. Cell staining was done with CellRox Orange followed by flow cytometry to quantify ROS.Results: ROS count for Vitamin C pre-treated red cells was significantly lower than matched controls (p<0.001). Average ROS count for 80uM test samples was 27.5/ul (95% CI, 17.5 to 72.5) and for 100uM 3.9/ul (95% CI, 1.9 to 5.9). Male gender was significantly associated with elevated baseline ROS count (p=0.03).Conclusion:Vitamin C reduced ROS formation in HbSS cells. Future studies should focus on a role for Vitamin C as a safe, cheap addition to maintenance therapy of sickle cell patients.

JRK’S ANTI- COFF – AN EFFECTIVE REMEDY FOR REACTIVE OXYGEN SPECIES SCAVENGING

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Soundharya R ◽  
Aruna V ◽  
Amruthavalli GV ◽  
Gayathri R
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Therapeutic Benefit ◽  
Blood Purification ◽  
Ocimum Sanctum ◽  
Oxygen Species ◽  
Leucas Aspera ◽  
Anti Oxidant ◽  
Effective Remedy ◽  
Acalypha Indica

JRK’s Anti-coff was studied for reactive oxygen species scavenging benefit inorder to establish the anti-oxidant therapeutic benefit. Findings clearly show that the herbs used in JRK’s Anti-coff such as Anisochilus carnosus, Leucas aspera, Ocimum sanctum, Solanum trilobatum, Acalypha indica, and Adhatoda vasica showed antioxidant effect. We have attempted to co-relate the above benefit of JRK’s Anti-coff with the blood purification benefit postulated by Ayurveda and Siddha. Further the importance of blood purification for the treatment of several diseases is discussed. Keywords: Ocimum sanctum, ROS, Blood purification effect, JRK’s Anti- coff

Quality and reactive oxygen species of extended canine semen after vitamin C supplementation

2008 ◽  
Vol 70 (5) ◽  
pp. 827-835 ◽  
Author(s):  
A.J. Michael ◽  
C. Alexopoulos ◽  
E.A. Pontiki ◽  
D.J. Hadjipavlou-Litina ◽  
Ph. Saratsis ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Vitamin C ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Vitamin C Supplementation

scholarly journals Alteration of the Soluble Guanylate Cyclase System in the Vascular Wall of Lead-Induced Hypertension in Rats

2001 ◽  
Vol 12 (12) ◽  
pp. 2594-2600 ◽  
Author(s):  
María Marques ◽  
Inmaculada Millás ◽  
Ana Jiménez ◽  
Elena García-Colis ◽  
Juan A. Rodriguez-Feo ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Vitamin C ◽  
Sodium Nitroprusside ◽  
Guanylate Cyclase ◽  
Lead Exposure ◽  
Soluble Guanylate Cyclase ◽  
Reactive Oxygen ◽  
Vascular Wall ◽  
No Synthase ◽  
Oxygen Species

ABSTRACT. Low-level lead exposure is a known cause of hypertension that has been associated with increased reactive oxygen species activity and endothelial-dependent vasorelaxation impairment. The effect of lead exposure on the vascular nitric oxide (NO)/cyclic guanocine monophosphate (cGMP) system was analyzed. Wistar rats were exposed to 5 ppm lead acetate in the drinking water during 30 d. Mean arterial BP increased significantly in the lead-treated rats. Relaxation to both acetylcholine and sodium nitroprusside (SNP) was reduced in lead-treated rats; however, the vascular wall of lead-administered rats showed an increased expression of endothelial NO synthase. The expression of both subunits (α1and β1) of soluble guanylate cyclase (sGC) and the cGMP accumulated in the vascular wall were decreased in lead-treated rats. Cotreatment of lead with vitamin C (3 mmol/L) prevented the increase on mean arterial BP, improved the relaxation to both acetylcholine and sodium nitroprusside, and restored the normal expression of endothelial NO synthase and sGC proteins in the vascular wall. In conclusion, lead exposure altered both the endothelium-dependent and -independent relaxing response and induced a reduced expression of sGC in the vascular wall. These effects were abrogated with the antioxidant vitamin C, which suggests the involvement of reactive oxygen species in the regulation of the NO/cGMP relaxing system in the vascular wall of lead-treated rats.

Effects of antipsychotics and vitamin C on the formation of reactive oxygen species

2009 ◽  
Vol 24 (10) ◽  
pp. 1499-1504 ◽  
Author(s):  
P. Heiser ◽  
O. Sommer ◽  
AJ Schmidt ◽  
HW Clement ◽  
A. Hoinkes ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Vitamin C ◽  
Reactive Oxygen ◽  
Oxygen Species
Sign in / Sign up

Export Citation Format

Share Document