Effect of Age and Glucocorticoid Administration on the Proteolytic Activity of Gastric Mucosa: a Comparative Study in the Young Rat, Calf and Piglet2,3

1983 ◽  
Vol 57 (1) ◽  
pp. 74-81 ◽  
Author(s):  
G. Pelletier ◽  
J. Lanoë ◽  
M. Filion ◽  
J. Dunnigan
Keyword(s):  
Gastric Mucosa ◽  
Comparative Study ◽  
Proteolytic Activity ◽  
Glucocorticoid Administration ◽  
Young Rat ◽  
Effect Of Age

Sem Observations in Gastric Mucosa Exposed to Progressive Enzymatic Etching

1980 ◽  
Vol 38 ◽  
pp. 570-571
Author(s):  
C.A.E. Lemmi ◽  
D. Booth ◽  
G.E. Adomian
Keyword(s):  
Gastric Mucosa ◽  
Critical Point ◽  
Proteolytic Activity ◽  
Etching Process ◽  
Cellular Types

In order to enrich populations of homogeneous cellular types we dissociated gastric mucosa by enzymatic techniques. In addition, we used SEM to monitor the progressive etching of the mucosa. Two enzymes were tested: collagenase III with minimum proteolytic activity and Pronase with broader proteolytic effects. The gastric mucosa was exposed to the effect of the enzymes using everted stomach preparations. In this way the digestive action occured progressively from the lumen of the stomach toward the base of the glands. This “etching” process could be monitored conveniently by SEM. After incubation for periods varying from 30 to 210 minutes the tissues were stretched on dental wax, fixed in 2 % glutaralheyde, post-fixed in osmium, dehydrated, critical point dryed and coated with gold. A model MSM-5 “Mini-SEM” was used for observation. Gentle uncurling of the preparation before coating with gold produced fractures which revealed the structure of the gastric glandsin more detail.

Lethality of Candida strains as influenced by the host

1975 ◽  
Vol 21 (5) ◽  
pp. 648-654 ◽  
Author(s):  
Cora G. Saltarelli ◽  
Kathi Ann Gentile ◽  
Susan C. Mancuso
Keyword(s):  
Candida Albicans ◽  
Statistical Analysis ◽  
Comparative Study ◽  
Proteolytic Activity ◽  
Germ Tube ◽  
Culture Media ◽  
Tube Formation ◽  
Nutritional Requirements ◽  
Morphological Mutant ◽  
Mutant Strains

A comparative study of the pathogenicity of Candida albicans morphological mutant strains was made to relate chlamydospore production, germ tube formation, and proteolytic activity to candidiasis in mice. It was observed that the mycelium strains were more lethal than the yeast-like strains and that neither chlamydospore production, germ tube formation, nor nutritional requirements was related to pathogenicity in mice. Statistical analysis indicated that the culture media of the organisms and the strain and sex of the mice into which the cells were injected were important factors in the development of pathogenicity.

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