Assessment of genotoxic effects in one-and two-cell mouse embryos in vivo and in vitro using comet assay

Introduction. Consistency of fluoride excess in the human environment and professional contact with fluoride is an actual and underestimated problem. Fluoride ion is able to displace the hydroxyl group in calcium hydroxyapatites, forming stable crystals of mixed form of apatites, inducing bone pathology, fluorosis. Despite the high prevalence of fluorosis, there are only a few studies discussing the ability of fluoride ion to increase the level of genotoxic effects. At the same time, such studies are in high demand in connection with a direct correlation between genetic instability and the risk of carcinogenesis. Material and methods. A literature search was conducted according the following queries: “fluoride, fluoride ion, fluorides, DNA damage, genetic damage, genotoxicity.” The search was conducted on the databases PubMed, MEDLINE, Embase and Google Scholar for various articles (all publications until June 2018). All publications were analyzed and included in this review. Results.The present review examines the results of studies aimed at investigation of the ability of fluoride to induce DNA damage, published since the 50-s of 20th century to the present. The analyse of data about genotoxic and mutagenic properties of fluorine observed in In vitro and In vivo studies is provided. It is summarized that at concentrations of sodium fluoride in drinking water of more than 1 mM, fluoride ion has the ability to induce DNA damage and increase the frequency of clastogenic effects in humans and large monkeys. At the same time, for a significant increase in genotoxic effects in rodents, large concentrations of fluorides are required. The main hypotheses about the mechanisms of the fluoride genotoxic properties are described. Conclusion. Considering results published nowadays, it can be noted that fluoride ion obviously showes a number of genotoxic features and can have mutagenic properties in case of chronic and direct contact with cellular objects. It remains questionable issue about genotoxic risk accompanied human contact with fluoride compounds.

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Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay

Genetika ◽

10.2298/gensr1602617d ◽

2016 ◽

Vol 48 (2) ◽

pp. 617-627

Author(s):

Stefan Dacic ◽

Ninoslav Djelic ◽

Milena Radakovic ◽

Nada Lakic ◽

Aleksandar Veselinovic ◽

...

Keyword(s):

Dna Damage ◽

Comet Assay ◽

Human Lymphocytes ◽

Negative Control ◽

In Vivo Studies ◽

Halogen Lamp ◽

Genotoxic Effects ◽

Significant Difference ◽

Isolated Human Lymphocytes

Certain in vivo studies have shown that the application of adhesives directly onto the open pulp or on a thin layer of dentin causes inflammation and pulpal abscesses. This reaction is related to toxic effects of monomers from adhesives. It has been confirmed that after proper illumination the adhesives become less toxic. The aim of the study was to examine genotoxicity of non-polymerised, partly polymerised and polymerised adhesives on isolated human lymphocytes using the alkaline Comet assay. Adper Single bond2 and Adper Easy One/3M ESPE adhesive photopolymerisation was performed by Elipar Highlight 3M ESPE halogen lamp for 0, 10 and 40 sec, at final concentrations of 100, 200, 500 and 1000 ?g/mL. With both adhesives, photopolymerisation at 0 and 10 seconds showed statistically significant increase in DNA damage in comparision to the negative control (solvent). On the other hand, after 40 seconds of photopolymerisation of both adhesives in all tested concentrations, the degree of DNA damage in Comet assay had no significant difference (P>0.05, ?2 test) compared to the negative control. Therefore, only the 40 seconds of photopolymerisation prevented genotoxic effects of both adhesives in the Comet assay.

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The Comet Assay: Assessment of In Vitro and In Vivo DNA Damage

Methods in Molecular Biology - Genotoxicity Assessment ◽

10.1007/978-1-4939-9646-9_12 ◽

2019 ◽

pp. 237-257 ◽

Cited By ~ 2

Author(s):

Mahima Bajpayee ◽

Ashutosh Kumar ◽

Alok Dhawan

Keyword(s):

Dna Damage ◽

Comet Assay

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DNA Alkylation Damage as a Sensor of Nitrosative Stress in Mycobacterium tuberculosis

Infection and Immunity ◽

10.1128/iai.71.2.997-1000.2003 ◽

2003 ◽

Vol 71 (2) ◽

pp. 997-1000 ◽

Cited By ~ 33

Author(s):

Steven I. Durbach ◽

Burkhard Springer ◽

Edith E. Machowski ◽

Robert J. North ◽

K. G. Papavinasasundaram ◽

...

Keyword(s):

Dna Damage ◽

Mycobacterium Tuberculosis ◽

Nitrosative Stress ◽

Dna Alkylation ◽

Genotoxic Effects ◽

Alkylation Damage ◽

Damage Repair

ABSTRACT One of the cellular consequences of nitrosative stress is alkylation damage to DNA. To assess whether nitrosative stress is registered on the genome of Mycobacterium tuberculosis, mutants lacking an alkylation damage repair and reversal operon were constructed. Although hypersensitive to the genotoxic effects of N-methyl-N′-nitro-N-nitrosoguanidine in vitro, the mutants displayed no phenotype in vivo, suggesting that permeation of nitrosative stress to the level of cytotoxic DNA damage is restricted.

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The Comet Assay: Assessment of In Vitro and In Vivo DNA Damage

Methods in Molecular Biology - Genotoxicity Assessment ◽

10.1007/978-1-62703-529-3_17 ◽

2013 ◽

pp. 325-345 ◽

Cited By ~ 20

Author(s):

Mahima Bajpayee ◽

Ashutosh Kumar ◽

Alok Dhawan

Keyword(s):

Dna Damage ◽

Comet Assay

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GENOTOXIC PROPERTIES OF FLUORINES (REVIEW)

Hygiene and Sanitation ◽

10.33029/0016-9900-2020-99-3-253-258 ◽

2020 ◽

Vol 99 (3) ◽

pp. 253-258

Author(s):

E. E. Kalyuzhnaya ◽

A. Yu. Prosekov ◽

Valentin P. Volobaev

Keyword(s):

Dna Damage ◽

In Vivo Studies ◽

Hydroxyl Group ◽

Fluoride Ion ◽

Genotoxic Effects ◽

Human Environment ◽

Human Contact ◽

Professional Contact

Introduction. Consistency of fluoride excess in the human environment and professional contact with fluoride is an actual and underestimated problem. Fluoride ion is able to displace the hydroxyl group in calcium hydroxyapatites, forming stable crystals of mixed form of apatites, inducing bone pathology, fluorosis. Despite the high prevalence of fluorosis, there are only a few studies discussing the ability of fluoride ion to increase the level of genotoxic effects. At the same time, such studies are in high demand in connection with a direct correlation between genetic instability and the risk of carcinogenesis. Material and methods. A literature search was conducted according the following queries: “fluoride, fluoride ion, fluorides, DNA damage, genetic damage, genotoxicity.” The search was conducted on the databases PubMed, MEDLINE, Embase and Google Scholar for various articles (all publications until June 2018). All publications were analyzed and included in this review. Results.The present review examines the results of studies aimed at investigation of the ability of fluoride to induce DNA damage, published since the 50-s of 20th century to the present. The analyse of data about genotoxic and mutagenic properties of fluorine observed in In vitro and In vivo studies is provided. It is summarized that at concentrations of sodium fluoride in drinking water of more than 1 mM, fluoride ion has the ability to induce DNA damage and increase the frequency of clastogenic effects in humans and large monkeys. At the same time, for a significant increase in genotoxic effects in rodents, large concentrations of fluorides are required. The main hypotheses about the mechanisms of the fluoride genotoxic properties are described. Conclusion. Considering results published nowadays, it can be noted that fluoride ion obviously showes a number of genotoxic features and can have mutagenic properties in case of chronic and direct contact with cellular objects. It remains questionable issue about genotoxic risk accompanied human contact with fluoride compounds.

Download Full-text