Effects of modified gravity coupled with mechanical stimulation on molecular signal transduction and target gene transcription in 3D osteon cell network.

2017 ◽  
Author(s):  
Cassandra M. Juran
Keyword(s):  
Signal Transduction ◽  
Gene Transcription ◽  
Mechanical Stimulation ◽  
Modified Gravity ◽  
Target Gene ◽  
Cell Network ◽  
Molecular Signal ◽  
Target Gene Transcription

scholarly journals β-catenin-Mediated Wnt Signal Transduction Proceeds Through an Endocytosis-Independent Mechanism

2020 ◽  
Author(s):  
Ellen Youngsoo Rim ◽  
Leigh Katherine Kinney ◽  
Roel Nusse
Keyword(s):  
Signal Transduction ◽  
Gene Transcription ◽  
Wnt Pathway ◽  
Target Gene ◽  
Single Cells ◽  
Receptor Endocytosis ◽  
Pathway Activation ◽  
Essential Components ◽  
Wnt Signal ◽  
Target Gene Transcription

The Wnt pathway is a key intercellular signaling cascade that regulates development, tissue homeostasis, and regeneration. However, gaps remain in our understanding of the molecular events that take place between ligand-receptor binding and target gene transcription. Here we used a novel tool for quantitative, real-time assessment of endogenous pathway activation, measured in single cells, to answer an unresolved question in the field – whether receptor endocytosis is required for Wnt signal transduction. We combined knockdown or knockout of essential components of Clathrin-mediated endocytosis with quantitative assessment of Wnt signal transduction in mouse embryonic stem cells (mESCs). Disruption of Clathrin-mediated endocytosis did not affect accumulation and nuclear translocation of β-catenin, as measured by single-cell live imaging of endogenous β-catenin, and subsequent target gene transcription. Disruption of another receptor endocytosis pathway, Caveolin-mediated endocytosis, did not affect Wnt pathway activation either. These results, confirmed in multiple cell lines, suggest that endocytosis is not a general requirement for Wnt signal transduction. We show that off-target effects of a drug used to inhibit endocytosis may be one source of the discrepancy among reports on the role of endocytosis in Wnt signaling.

scholarly journals Specific expression of the Fusarium transposon Fot1 and effects on target gene transcription

1999 ◽  
Vol 31 (5) ◽  
pp. 1373-1383 ◽  
Author(s):  
Francine Deschamps ◽  
Thierry Langin ◽  
Patricia Maurer ◽  
Catherine Gerlinger ◽  
Beatrice Felenbok ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Target Gene ◽  
Specific Expression ◽  
Target Gene Transcription

PS18 - 87. A novel FPLD-associated PPARgamma mutant (E379K) displays a selective defect in target gene transcription

2012 ◽  
Vol 10 (3) ◽  
pp. 161-161
Author(s):  
Arjen Koppen ◽  
David Cassiman ◽  
Mariette Kranendonk ◽  
Marian Groot Koerkamp ◽  
Nicole Hamers ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Target Gene ◽  
Target Gene Transcription

Antidiabetic sulfonylureas modulate farnesoid X receptor activation and target gene transcription

2010 ◽  
Vol 2 (4) ◽  
pp. 575-586 ◽  
Author(s):  
Ramona Steri ◽  
Mahmut Kara ◽  
Ewgenij Proschak ◽  
Dieter Steinhilber ◽  
Gisbert Schneider ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Target Gene ◽  
Receptor Activation ◽  
Farnesoid X Receptor ◽  
Target Gene Transcription

scholarly journals KDM3 epigenetically controls tumorigenic potentials of human colorectal cancer stem cells through Wnt/β-catenin signalling

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Jiong Li ◽  
Bo Yu ◽  
Peng Deng ◽  
Yingduan Cheng ◽  
Yongxin Yu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Gene Transcription ◽  
Target Gene ◽  
Histone Demethylases ◽  
Human Colorectal Cancer ◽  
Tumorigenic Potential ◽  
Colorectal Cancer Stem Cells ◽  
Target Gene Transcription

Abstract Human colorectal cancer stem cells (CSCs) are tumour initiating cells that can self-renew and are highly tumorigenic and chemoresistant. While genetic mutations associated with human colorectal cancer development are well-known, little is known about how and whether epigenetic factors specifically contribute to the functional properties of human colorectal CSCs. Here we report that the KDM3 family of histone demethylases plays an important role in tumorigenic potential and survival of human colorectal CSCs by epigenetically activating Wnt target gene transcription. The depletion of KDM3 inhibits tumorigenic growth and chemoresistance of human colorectal CSCs. Mechanistically, KDM3 not only directly erases repressive H3K9me2 marks, but also helps to recruit histone methyltransferase MLL1 to promote H3K4 methylation, thereby promoting Wnt target gene transcription. Our results suggest that KDM3 is a critical epigenetic factor in Wnt signalling that orchestrates chromatin changes and transcription in human colorectal CSCs, identifying potential therapeutic targets for effective elimination of CSCs.

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