scholarly journals Silicon Photomultipliers as a Low-Cost Fluorescence Detector for Capillary Electrophoresis

2020 ◽  
Author(s):  
Brae Petersen ◽  
Luke Gallion ◽  
Nancy Allbritton
Keyword(s):  
Capillary Electrophoresis ◽  
Open Source ◽  
Dynamic Range ◽  
Low Cost ◽  
Single Cells ◽  
Detection Limits ◽  
High Sensitivity ◽  
Small Sample ◽  
Fluorescence Detector ◽  
Silicon Photomultipliers

Capillary electrophoresis (CE) is a highly efficient separation method capable of handling small sample volumes (~pL) and low (~yoctomole) detection limits, and as such is ideal for applications that require high sensitivity such as single-cell analysis. Low-cost CE instrumentation is quickly expanding but low-cost, open-source fluorescence detectors with ultra-sensitive detection limits are lacking. Silicon photomultipliers (SiPM) are inexpensive, low-footprint detectors with the potential to fill the role as a detector when cost, size, and customization are important. In this work we demonstrate the use of a SiPM in CE with zeptomolar detection limits and a dynamic range spanning five orders of magnitude, comparable to photomultiplier detectors. We characterize the performance of the SiPM as a highly sensitive detector by measuring enzyme activity in single cells. This simple, small footprint, and low-cost (<$130) light detection circuit will be beneficial for open-source, portable, and budget friendly instrumentation requiring high sensitivity.<br>

Silicon Photomultipliers as a Low-Cost Fluorescence Detector for Capillary Electrophoresis

2020 ◽  
Author(s):  
Brae Petersen ◽  
Luke Gallion ◽  
Nancy Allbritton
Keyword(s):  
Capillary Electrophoresis ◽  
Open Source ◽  
Dynamic Range ◽  
Low Cost ◽  
Single Cells ◽  
Detection Limits ◽  
High Sensitivity ◽  
Small Sample ◽  
Fluorescence Detector ◽  
Silicon Photomultipliers

Capillary electrophoresis (CE) is a highly efficient separation method capable of handling small sample volumes (~pL) and low (~yoctomole) detection limits, and as such is ideal for applications that require high sensitivity such as single-cell analysis. Low-cost CE instrumentation is quickly expanding but low-cost, open-source fluorescence detectors with ultra-sensitive detection limits are lacking. Silicon photomultipliers (SiPM) are inexpensive, low-footprint detectors with the potential to fill the role as a detector when cost, size, and customization are important. In this work we demonstrate the use of a SiPM in CE with zeptomolar detection limits and a dynamic range spanning five orders of magnitude, comparable to photomultiplier detectors. We characterize the performance of the SiPM as a highly sensitive detector by measuring enzyme activity in single cells. This simple, small footprint, and low-cost (<$130) light detection circuit will be beneficial for open-source, portable, and budget friendly instrumentation requiring high sensitivity.<br>

scholarly journals Silicon Photomultipliers as a Low-Cost Fluorescence Detector for Capillary Electrophoresis

2020 ◽  
Vol 92 (20) ◽  
pp. 13683-13687 ◽  
Author(s):  
Brae V. Petersen ◽  
Luke Gallion ◽  
Nancy L. Allbritton
Keyword(s):  
Capillary Electrophoresis ◽  
Low Cost ◽  
Fluorescence Detector ◽  
Silicon Photomultipliers

scholarly journals A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination

2022 ◽  
Author(s):  
Karel Miettinen ◽  
Nattawat Leelahakorn ◽  
Aldo Almeida ◽  
Yong Zhao ◽  
Lukas Hansen ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Low Cost ◽  
Real Life ◽  
High Sensitivity ◽  
Structural Diversity ◽  
High Throughput Analysis ◽  
Synthetic Drugs ◽  
Point Of Use ◽  
Whole Cell Biosensor ◽  
G Protein Coupled

Abstract The decriminalization of cannabis and the growing interest in cannabinoids as therapeutics require efficient methods to discover novel compounds and monitor cannabinoid levels in human samples and products. However, current methods are limited by the structural diversity of the active compounds. Here, we construct a G-protein coupled receptor-based yeast whole-cell biosensor, optimize it to achieve high sensitivity and dynamic range, and prove its effectiveness in three real-life applications. First, we screen a library of compounds to discover two novel agonists and four antagonists and demonstrate that our biosensor can democratize GPCR drug discovery by enabling low-cost high-throughput analysis using open-source automation. Subsequently, we bioprospect 54 plants to discover a novel phytocannabinoid, dugesialactone. Finally, we develop a robust portable device, analyze body-fluid samples, and confidently detect illicit synthetic drugs like “Spice”/“K2”. Taking advantage of the extensive sensing repertoire of GPCRs, this technology can be extended to detect numerous other compounds.

scholarly journals Fast infectious diseases diagnostics based on microfluidic biochip system

2017 ◽  
Vol 10 (02) ◽  
pp. 1650044 ◽  
Author(s):  
Qin Huang ◽  
Shanqiao Han ◽  
Yan Zhang ◽  
Yue Kou ◽  
Xiaohang Zhao ◽  
...  
Keyword(s):  
Infectious Diseases ◽  
Molecular Diagnostics ◽  
Microfluidic Chip ◽  
Pathogen Detection ◽  
Low Cost ◽  
High Sensitivity ◽  
Optical Diffraction ◽  
Fluorescence Detector ◽  
Total Analysis System ◽  
Amplification Method

Molecular diagnostics is one of the most important tools currently in use for clinical pathogen detection due to its high sensitivity, specificity, and low consume of sample and reagent is keyword to low cost molecular diagnostics. In this paper, a sensitive DNA isothermal amplification method for fast clinical infectious diseases diagnostics at aM concentrations of DNA was developed using a polycarbonate (PC) microfluidic chip. A portable confocal optical fluorescence detector was specifically developed for the microfluidic chip that was capable of highly sensitive real-time detection of amplified products for sequence-specific molecular identification near the optical diffraction limit with low background. The molecular diagnostics of Listeria monocytogenes with nucleic acid extracted from stool samples was performed at a minimum DNA template concentration of 3.65[Formula: see text]aM, and a detection limit of less than five copies of genomic DNA. Contrast to the general polymerase chain reaction (PCR) at eppendorf (EP) tube, the detection time in our developed method was reduced from 1.5[Formula: see text]h to 45[Formula: see text]min for multi-target parallel detection, the consume of sample and reagent was dropped from 25[Formula: see text][Formula: see text]L to 1.45[Formula: see text][Formula: see text]L. This novel microfluidic chip system and method can be used to develop a micro total analysis system as a clinically relevant pathogen molecular diagnostics method via the amplification of targets, with potential applications in biotechnology, medicine, and clinical molecular diagnostics.

Sheath-Flow Cuvette for High-Sensitivity Laser-Induced Fluorescence Detection in Capillary Electrophoresis

2007 ◽  
Vol 61 (7) ◽  
pp. 777-779 ◽  
Author(s):  
Kimia Sobhani ◽  
David A. Michels ◽  
Norman J. Dovichi
Keyword(s):  
Stainless Steel ◽  
Capillary Electrophoresis ◽  
Carbonic Anhydrase ◽  
High Sensitivity ◽  
Laser Induced Fluorescence ◽  
Flow Chamber ◽  
Fluorescence Detector ◽  
Sheath Flow ◽  
Laser Induced Fluorescence Detection ◽  
Sheath Flow Cuvette

The sheath-flow cuvette is a key component in a high-sensitivity post-column laser-induced fluorescence detector for capillary electrophoresis. Most designs are based on commercial cuvettes originally manufactured for use in a flow cytometer. In these devices, a quartz flow chamber is held in a stainless-steel fixture that is difficult to machine and subjects the cuvette to a torque when sealed, which frequently leads to damage of the flow chamber. In this report we present a design for a cuvette that may easily be constructed. This design uses compression to hold and seal the quartz flow chamber without applying torque. The system produces detection limits (3σ) of 115 yoctomoles (70 copies) for FQ-labeled carbonic anhydrase.

scholarly journals sNucDrop-Seq: Dissecting cell-type composition and neuronal activity state in mammalian brains by massively parallel single-nucleus RNA-Seq

2017 ◽  
Author(s):  
Peng Hu ◽  
Emily Fabyanic ◽  
Zhaolan Zhou ◽  
Hao Wu
Keyword(s):  
Single Cell ◽  
Neuronal Activity ◽  
Low Cost ◽  
Single Cells ◽  
High Sensitivity ◽  
Droplet Microfluidics ◽  
Massively Parallel ◽  
Rna Seq ◽  
Single Nucleus

Massively parallel single-cell RNA sequencing can precisely resolve cellular diversity in a high-throughput manner at low cost, but unbiased isolation of intact single cells from complex tissues, such as adult mammalian brains, is challenging. Here, we integrate sucrose-gradient assisted nuclear purification with droplet microfluidics to develop a highly scalable single-nucleus RNA-Seq approach (sNucDrop-Seq), which is free of enzymatic dissociation and nucleus sorting. By profiling ~11,000 nuclei isolated from adult mouse cerebral cortex, we demonstrate that sNucDrop-Seq not only accurately reveals neuronal and non-neuronal subtype composition with high sensitivity, but also enables analysis of long non-coding RNAs and transient states such as neuronal activity-dependent transcription at single-cell resolution in vivo.

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