scholarly journals Detection of SARS-CoV-2 Viral Particles Using Direct, Reagent-Free Electrochemical Sensing

2020 ◽  
Author(s):  
Hanie Yousefi ◽  
alam mahmud ◽  
Dingran Chang ◽  
Jagotamoy Das ◽  
Surath Gomis ◽  
...  
Keyword(s):  
Electrode Surface ◽  
Gold Electrode ◽  
Direct Analysis ◽  
Electrochemical Sensing ◽  
New Method ◽  
Spike Protein ◽  
Positive Potential ◽  
Molecular Sensor ◽  
Viral Particles ◽  
Kinetics Of

This manuscript describes a new method that enables direct analysis of viral particles in unprocessed samples.Using an electrochemical readout method that requires no external reagents, we detect the SARS-CoV-2 virus in the saliva of infected patients.The approach relies on a molecular sensor tethered to the surface of a gold electrode that contains an antibody, specific to the targetof interest, which here is the SARS-CoV-2 S1 spike protein that is displayed on the viral capsule. The antibody is attached to the electrode using a negatively charged linker that is composed of DNA. When a positive potential is applied to the electrode, the sensor complex is attracted to the electrode surface. The kinetics of transport is measured using chronoamperometry and readout is possible based on the absense or precense of virus and its effect on the complex movevment on electrode surface.

scholarly journals Detection of SARS-CoV-2 Viral Particles Using Direct, Reagent-Free Electrochemical Sensing

2020 ◽  
Author(s):  
Hanie Yousefi ◽  
alam mahmud ◽  
Dingran Chang ◽  
Jagotamoy Das ◽  
Surath Gomis ◽  
...  
Keyword(s):  
Electrode Surface ◽  
Gold Electrode ◽  
Direct Analysis ◽  
Electrochemical Sensing ◽  
New Method ◽  
Spike Protein ◽  
Positive Potential ◽  
Molecular Sensor ◽  
Viral Particles ◽  
Kinetics Of

This manuscript describes a new method that enables direct analysis of viral particles in unprocessed samples.Using an electrochemical readout method that requires no external reagents, we detect the SARS-CoV-2 virus in the saliva of infected patients.The approach relies on a molecular sensor tethered to the surface of a gold electrode that contains an antibody, specific to the targetof interest, which here is the SARS-CoV-2 S1 spike protein that is displayed on the viral capsule. The antibody is attached to the electrode using a negatively charged linker that is composed of DNA. When a positive potential is applied to the electrode, the sensor complex is attracted to the electrode surface. The kinetics of transport is measured using chronoamperometry and readout is possible based on the absense or precense of virus and its effect on the complex movevment on electrode surface.

scholarly journals Detection of SARS-CoV-2 Viral Particles using Direct, Reagent-Free Electrochemical Sensing

2020 ◽  
Author(s):  
Hanie Yousefi ◽  
alam mahmud ◽  
Dingran Chang ◽  
Jagotamoy Das ◽  
Surath Gomis ◽  
...  
Keyword(s):  
Electrode Surface ◽  
Gold Electrode ◽  
Direct Analysis ◽  
Electrochemical Sensing ◽  
New Method ◽  
Spike Protein ◽  
Positive Potential ◽  
Molecular Sensor ◽  
Viral Particles ◽  
Kinetics Of

This manuscript describes a new method that enables direct analysis of viral particles in unprocessed samples.Using an electrochemical readout method that requires no external reagents, we detect the SARS-CoV-2 virus in the saliva of infected patients.The approach relies on a molecular sensor tethered to the surface of a gold electrode that contains an antibody, specific to the targetof interest, which here is the SARS-CoV-2 S1 spike protein that is displayed on the viral capsule. The antibody is attached to the electrode using a negatively charged linker that is composed of DNA. When a positive potential is applied to the electrode, the sensor complex is attracted to the electrode surface. The kinetics of transport is measured using chronoamperometry and readout is possible based on the absense or precense of virus and its effect on the complex movevment on electrode surface.

scholarly journals Detection of SARS-CoV-2 Viral Particles using Direct, Reagent-Free Electrochemical Sensing

2020 ◽  
Author(s):  
Hanie Yousefi ◽  
alam mahmud ◽  
Dingran Chang ◽  
Jagotamoy Das ◽  
Surath Gomis ◽  
...  
Keyword(s):  
Electrode Surface ◽  
Gold Electrode ◽  
Direct Analysis ◽  
Electrochemical Sensing ◽  
New Method ◽  
Spike Protein ◽  
Positive Potential ◽  
Molecular Sensor ◽  
Viral Particles ◽  
Kinetics Of

This manuscript describes a new method that enables direct analysis of viral particles in unprocessed samples.Using an electrochemical readout method that requires no external reagents, we detect the SARS-CoV-2 virus in the saliva of infected patients.The approach relies on a molecular sensor tethered to the surface of a gold electrode that contains an antibody, specific to the targetof interest, which here is the SARS-CoV-2 S1 spike protein that is displayed on the viral capsule. The antibody is attached to the electrode using a negatively charged linker that is composed of DNA. When a positive potential is applied to the electrode, the sensor complex is attracted to the electrode surface. The kinetics of transport is measured using chronoamperometry and readout is possible based on the absense or precense of virus and its effect on the complex movevment on electrode surface.

Kinetics of compact layer formation and growth of 1,10-phenanthroline at the electrode surface

1990 ◽  
Vol 87 ◽  
pp. 1597-1607 ◽  
Author(s):  
L Benedetti ◽  
M Borsari ◽  
C Fontanesi ◽  
G Battistuzzi Gavioli
Keyword(s):  
Electrode Surface ◽  
Layer Formation ◽  
Compact Layer ◽  
Kinetics Of

scholarly journals Detection of SARS-CoV-2 Viral Particles Using Direct, Reagent-Free Electrochemical Sensing

2021 ◽  
Vol 143 (4) ◽  
pp. 1722-1727
Author(s):  
Hanie Yousefi ◽  
Alam Mahmud ◽  
Dingran Chang ◽  
Jagotamoy Das ◽  
Surath Gomis ◽  
...  
Keyword(s):  
Electrochemical Sensing ◽  
Viral Particles
Sign in / Sign up

Export Citation Format

Share Document