A Single Polyphosphate Kinase-Based NTP Regeneration System Driving Cell-Free Protein Synthesis
Keyword(s):
<p>Current reconstituted cell-free protein synthesis systems regenerate the ATP and GTP required for peptide bond formation using phosphocreatine and three coupled kinase reactions. Here, we replace the three-kinase system with a single polyphosphate kinase, resulting in a comparable protein yield and enabling functional expression of a heat-sensitive enzyme at 37°C.</p>
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