scholarly journals Antioxidant and Anti-inflammatory activities of the flower extracts of Argemone mexicana L.

2020 ◽  
Vol 11 (1) ◽  
pp. 323-330
Author(s):  
Prabhakaran D ◽  
Rajeshkanna A ◽  
Senthamilselvi M M
Keyword(s):  
Nitric Oxide ◽  
Ethyl Acetate ◽  
Mononuclear Cells ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Powder Sample ◽  
Inflammatory Activity ◽  
Argemone Mexicana ◽  
Antioxidant Efficiency ◽  
Anti Inflammatory

To assess the antioxidant and anti-inflammatory activities of the powder sample derived from the ethyl acetate fraction of the floral Argemone mexicana L. For antioxidant efficiency, the floral extract was evaluated using 1, 1–diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay and the FRC (Ferric Reduction Capacity) assay. In vitro anti-inflammatory activity was assessed using human peripheral blood mononuclear cells (PBMC) induced by lipopolysaccharide (LPS) and the production method of nitric oxide (NO). The powder sample extracted from ethyl acetate fraction of the floral of Argemone Mexicana L showed good antioxidant activity with the comparative standard sample in scavenging DPPH radicals and in FRC assay. In the cell viability (PBMC influenced by LPS) method and the Nitric oxide (NO) assay, this sample showed even able anti-inflammative activities. Such results indicate a significant antioxidant and anti-inflammatory activity in the powder sample obtained from ethyl acetate fraction in the flower of Argemone mexicana L.                                                                                           

scholarly journals In vitro Antioxidant and Anti-inflammatory Activities of the Flower Extracts of Hibiscus vitifolius L

2019 ◽  
pp. 1-8
Author(s):  
D. Prabhakaran ◽  
A. Rajeshkanna ◽  
M. M. Senthamilselvi ◽  
S. Solomon
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Mononuclear Cells ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Powder Sample ◽  
Inflammatory Activity ◽  
Anti Inflammatory

Objective: To determine the antioxidant and anti-inflammatory activities of the solid powder extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L. Methods: The flower extract assessed for antioxidant activity using the 1,1–diphenyl-2-picryl-hydrazile (DPPH) radical scavenging assay and the reduced power assay was performed using the Ferric Reducing Capacity (FRC) assay. In vitro anti-inflammatory activity was assessed using human peripheral blood mononuclear cells (PBMC) induced by lipopolysaccharide (LPS) to test the production method of nitric oxide (NO). Results: The solid powder extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L showed good antioxidant activity in the scavenging DPPH radicals and the FRC assay compared to the standard sample. This powder sample also showed good anti-inflammatory activity in cell viability (LPS induced PBMC) assay and nitric oxide (NO) assay.  Conclusion: These results suggest that the powder sample extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L has substantial antioxidant and anti-inflammatory activity.

scholarly journals Anti-Inflammatory Activity of the Phlorotannin Trifuhalol A Using LPS-Stimulated RAW264.7 Cells Through NF-κB and MAPK Main Signaling Pathways

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1984979 ◽  
Author(s):  
Kasira Phasanasophon ◽  
Sang Moo Kim
Keyword(s):  
Nitric Oxide ◽  
Ethyl Acetate ◽  
Signaling Pathways ◽  
Ethyl Acetate Fraction ◽  
Inflammatory Activity ◽  
Raw264.7 Cells ◽  
No Production ◽  
Mitogen Activated Protein ◽  
Anti Inflammatory ◽  
Factor Α

Trifuhalol A, a phlorotannin, was extracted from Agarum cribrosum with ethyl acetate and fractionated using Sephadex LH-20 column chromatography (SF1-SF6). The ethyl acetate fraction (EAF) and SF5-containing trifuhalol A exhibited strong inhibitory activity against hyaluronidase. The anti-inflammatory activity of the phlorotannin, EAF, and SF5 was determined through the inhibition of nitric oxide (NO) production in lipopolysaccharide-stimulated RAW264.7 cells. Furthermore, the inhibition of NO production was validated by confirming the appreciable downregulation of inducible nitric oxide synthase expression. Agarum cribrosum phlorotannin also markedly suppressed the expression of cyclooxygenase-2, interleukin (IL)-1β, IL-6, and tumor necrosis factor-α. In addition, the anti-inflammatory action was verified by examining its effects on proinflammatory signaling pathways. The activation of nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs) was attenuated via the inhibition of NF-κB p-65, c-Jun N-terminal kinase, extracellular signal-regulated kinase 1/2, and p38 MAPK phosphorylation. Therefore, trifuhalol A is a potential source for either the prevention or the treatment of inflammation.

scholarly journals IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF THE FLOWER EXTRACTS OF OPUNTIA STRICTA

2019 ◽  
pp. 208-212
Author(s):  
PRABHAKARAN D ◽  
RAJESHKANNA A ◽  
SENTHAMILSELVI MM
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Inflammatory Activity ◽  
Assay Method ◽  
No Production ◽  
Dpph Radical ◽  
Anti Inflammatory

Objective: The objective of this study was to evaluate the antioxidant and anti-inflammatory activities of the solid powder obtained from the ethyl acetate fraction from the flower Opuntia stricta. Methods: The flower extract was evaluated for antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and reducing power assay was carried out by ferric-reducing capacity (FRC) assay method. The in vitro anti-inflammatory activity was evaluated using human peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide (LPS) to evaluate nitric oxide (NO) production method. Results: The solid powder obtained from the ethyl acetate fraction from the flower O. stricta showed a good antioxidant activity in scavenging DPPH radical and FRC assay with compared standard sample. This solid powder also showed good anti-inflammatory activity in cell viability (LPS-induced PBMCs) assay and NO assay. Conclusion: These results suggest that the solid powder obtained from the ethyl acetate fraction from the flower O. stricta has significant antioxidant and anti-inflammatory activities.

Anti-inflammatory activity of ethyl acetate fraction of the seeds of Brucea Javanica

2013 ◽  
Vol 147 (2) ◽  
pp. 442-446 ◽  
Author(s):  
Jianhong Yang ◽  
Shucai Li ◽  
Caifeng Xie ◽  
Haoyu Ye ◽  
Huan Tang ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Inflammatory Activity ◽  
Brucea Javanica ◽  
Anti Inflammatory

scholarly journals EVALUATION OF ETHANOLIC ROOT EXTRACT OF PARTHENIUM HYSTEROPHORUS LINN FOR ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY

2017 ◽  
pp. 194-197 ◽  
Author(s):  
Pankaj Lohumi ◽  
Tirath Kumar ◽  
Lipi Nogai
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Parthenium Hysterophorus ◽  
Inflammatory Activity ◽  
Root Extract ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Soxhlet Apparatus ◽  
Anti Inflammatory

Objective: The work is aimed to draw out the health beneficial properties of a weed (Parthenium hysterophorus Linn). The present work is organized to evaluate the antioxidant and anti-inflammatory activity of the ethanolic root extract of Parthenium hysterophorus Linn.Methods: In the present work the ethanolic extract was determined by using soxhlet apparatus. The antioxidant scavenging activity of this extract was determined by applying three different assay methods: (1) DPPH (1, 1-diphenyl-2-picryl hydrazyl) free radical method. (2) Nitric oxide scavenging assay and (3) Reducing power method. The anti-inflammatory activity was determined by in vivo method i.e. Carrageenan induced rat paw edema.Results: DPPH radical scavenging activities of the standard antioxidant and extracts were found to be increased in dose dependent manner. The percentage inhibition increases from 4.19% to 97.09% within the concentration range of 10µg/ml to 160µg/ml. Parthenium hysterophorus Linn effectively reduced the generation of nitric oxide radicals from sodium nitroprusside solution in a concentration dependent manner and percentage inhibition increases from 3.53% to 55.21% within the concentration range 10µg/ml to 160µg/ml. All the concentrations of extract significantly showed higher absorbance than the absorbance of control reaction (0.9705) in reducing power assay. A Higher absorbance indicates high reducing power due to the formation of reduced intermediates. Parthenium hysterophorus Linn showed highly significant anti-inflammatory activity at a dose of 200 mg/kg and the lesser effect was observed at 100 mg/kg with the percentage change in paw volume at 0 min, 30 min, 60 min, 90 min and 120 min.Conclusion: Thus, from above experimental observations, it can be stated that Parthenium is a natural antioxidant and bearing anti-inflammatory activity. 

scholarly journals ANTIOXIDATIVE ACTIVITIES AND CHEMICAL CONSTITUENTS OF THE ETHYL ACETATE EXTRACT FROM JASMINUM UNDULATUM KER-GAWL

2011 ◽  
Vol 14 (2) ◽  
pp. 50-57
Author(s):  
Anh Thi Tran ◽  
Binh Thanh Nguyen ◽  
Hoai Thi Cam Ho ◽  
Huong Dang Thien Bui ◽  
Mai Thi Thanh Nguyen
Keyword(s):  
Nitric Oxide ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Antioxidative Activity ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

From the total crude ethanol extract of Jasminum undulatum Ker Gawl.’s leaves and stems, five fractionss were obtained by partitioning with petroleum ether, chloroform, ethyl acetate and n-butanol solvents. These five fractions were investigated for antioxidative activity using the DPPH radical scavenging and nitric oxide-inhibitory assay. All the fractions showed antioxidative activity except the petroleum ether fraction. Among the fractionss, the ethyl acetate fraction was the most potent fraction in both assays with the SC50 values of 5.30 μg/ml and 80.90 μg/ml, respectively. Further investigation on the eight sub-fractions isolated and extracted from the ethyl acetate fraction showed that one of these sub-fractions, the TE6 sub-fraction, showed the most significant antioxidative activity with the SC50 values of 3.15 μg/ml and 61.83 μg/ml respectively in the DPPH radical scavenging and nitric oxide-inhibitory assay. From the TE4 and TE6 sub-fractions, three compounds were isolated, including p-tyrosol (1), protocatechuic acid (2) and hydroxytyrosol (3). The structure of those compounds were elucidated by spectrometric methods IR, MS, 1D-NMR, and 2D-NMR.

scholarly journals Evaluation of Antioxidant, Analgesic and Anti-Inflammatory Activity of Hydro - Alcoholic Extract and Ethyl Acetate Fraction of Withania Coagulans Dunal

2011 ◽  
Vol 4 (5) ◽  
pp. 530-533
Author(s):  
Md.Akram Minhaj ◽  
◽  
Dr.S.R Mir Dr.S.R Mir ◽  
Prof.Mohd Ali Prof.Mohd Ali ◽  
Md. Waris Md. Waris ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Inflammatory Activity ◽  
Alcoholic Extract ◽  
Withania Coagulans ◽  
Anti Inflammatory

scholarly journals Identification and in vitro Evaluation of Lipids from Sclerotia of Lignosus rhinocerotis for Antioxidant and Anti-neuroinflammatory Activities

2016 ◽  
Vol 11 (10) ◽  
pp. 1934578X1601101 ◽  
Author(s):  
Neeranjini Nallathamby ◽  
Lee Guan Serm ◽  
Jegadeesh Raman ◽  
Sri Nurestri Abd Malek ◽  
Sharmili Vidyadaran ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Bv2 Cells ◽  
Lignosus Rhinocerotis

Lignosus rhinocerotis (Cooke) Ryvarden (Tiger milk mushroom) is traditionally used to treat inflammation triggered symptoms and illnesses such as cough, fever and asthma. The present study evaluated the in vitro antioxidant, cytotoxic and anti-neuroinflammatory activities of the extract and fractions of sclerotia powder of L. rhinocerotis on brain microglial (BV2) cells. The ethyl acetate fraction had a total phenolic content of 0.30 ± 0.11 mg GAE/g. This fraction had ferric reducing capacity of 61.8 ± 1.8 mg FSE/g, ABTS•+ scavenging activity of 36.8 ± 1.8 mg TE/g and DPPH free radical scavenging activity of 21.8% ± 0.7. At doses ranging from 0.1 μg/mL – 100 μg/mL, the extract and fractions were not cytotoxic to BV2 cells. At 100 μg/mL, the crude hydroethanolic extract and the ethyl acetate fraction elicited the highest nitric oxide reduction activities of 68.7% and 58.2%, respectively. Linoleic and oleic acids were the major lipid constituents in the ethyl acetate fraction based on FID and GC-MS analysis. Linoleic acid reduced nitric oxide production and down regulated the expression of neuroinflammatory iNOS and COX2 genes in BV2 cells.

scholarly journals PHYTOCHEMICAL SCREENING AND ANTI-INFLAMMATORY ACTIVITY OF FRACTIONS FROM SAMBUNG RAMBAT (MICANIA CORDATA) LEAF

2018 ◽  
Vol 11 (8) ◽  
pp. 199
Author(s):  
Zola Efa Harnis ◽  
Panal Sitorus ◽  
Rosidah Rosidah
Keyword(s):  
Ethyl Acetate ◽  
Screening Method ◽  
Ethyl Acetate Fraction ◽  
Inflammatory Activity ◽  
Phytochemical Screening ◽  
Paw Edema ◽  
Water Solvent ◽  
Phytochemical Compounds ◽  
Anti Inflammatory

Objective: The present study is to evaluate the anti-inflammatory activity of various fractions from Sambung Rambat leaf (Micania cordata) using ethyl acetate, hexane, and water solvent as the solvent.Methods: Investigation of phytochemical compound was using standard phytochemical screening method, while the anti-inflammatory was using the carrageenan-induced paw edema method in animal (rats) model.Results: The phytochemical screening showed that the fractions of M. cordata had a lot of phytochemical compounds such as flavonoids, glycosides, steroids, triterpenoids, tannins, and saponins. Anti-inflammatory assessment showed that the strongest activity of anti-inflammatory produced by ethyl acetate fraction with 200 mg/kg BW dose.Conclusion: This investigation could be concluded that ethyl acetate fraction of M. cordata might be a potential for the treatment of inflammatory.

Chemical Compositions, Anti-Inflammatory, Antiproliferative and Radical-Scavenging Activities of Actinidia callosa var. ephippioides

2012 ◽  
Vol 40 (05) ◽  
pp. 1047-1062 ◽  
Author(s):  
Jung-Chun Liao ◽  
Jeng-Shyan Deng ◽  
Chuan-Sung Chiu ◽  
Shyh-Shyun Huang ◽  
Wen-Chi Hou ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Medicinal Plant ◽  
Radical Scavenging ◽  
Bioactive Compound ◽  
Ethyl Acetate Fraction ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Chemical Compositions ◽  
No Production ◽  
Anti Inflammatory

Oxidative stress and inflammation are related to several chronic diseases including cancer. Actinidia callosa var. ephippioides (ACE) is a special folk medicinal plant from Taiwan. The aim of this study is to evaluate the antioxidant, anti-inflammatory, and antiproliferative activities of the methanol extract and fractions from the stem of ACE. Trolox Equivalent Antioxidant Capacity (TEAC), 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, total phenolic content, flavonoid content, inhibition on nitric oxide (NO) productions by LPS-induced RAW264.7 cell, and on lung cancer cell proliferation were employed. Among all fractions, ethyl-acetate fraction (EA-ACE) showed higher TEAC, DPPH radical scavenging activities, polyphenol and flavonoid contents, respectively. EA-ACE also decreased the LPS-induced NO production and expressions of inducible nitric-oxide synthase (iNOS) in RAW264.7 cells. EA-ACE had the highest antiproliferative activity with an IC50 (The concentrations required for inhibition of 50% of cell viability) of 469.17 ± 3.59 μg/mL. Catechin also had good effects in the antioxidant and anti-inflammatory activities. Catechin might be an important bioactive compound in the stem of ACE. The above experimental data indicated that the stem of ACE is a potent antioxidant medicinal plant, and such efficacy may be mainly attributed to its polyphenolic compounds.

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