scholarly journals Nitrate Reductase Nanoparticles: Synthesis and Characterization

2020 ◽  
Vol 11 (3) ◽  
pp. 4583-4589
Author(s):  
Shelly ◽  
Minakshi Sharma
Keyword(s):  
Nitrate Reductase ◽  
Protein Production ◽  
Electrochemical Impedance ◽  
Catalytic Properties ◽  
Spectroscopic Studies ◽  
Serum Samples ◽  
Physical Chemical ◽  
Transmission Electron ◽  
Uv Visible ◽  
Electrochemical Impedance Spectroscopic

Nanoparticles of enzyme Nitrate reductase (NaR) a soluble homodimer enzyme of ∼100 kDa polypeptide with cofactors – FAD, heme-molybdopterin (Mo-MPT) and electron donor NAD(P)H, catalyses the reduction of nitrate to nitrite has been synthesised. Nanoparticles of Nitrate reductase enzyme have been prepared by chemical desolvation method including glutaraldehyde cross-linking to form the nanoaggregate. Characterisation of NaR nanoparticles has been made by Transmission Electron Microscopy (TEM), UV-Visible Spectroscopy and by electrochemical Impedance Spectroscopic Study (EIS). TEM study revealed the size of globular aggregated was in the range of 20–30 nm. UV Visible spectroscopic studies depicted that the absorption of NaR NPS is much higher at 560 nm than that of the free enzyme, which showed maximum absorption at 540 nm. NaR NPs aggregates formed were more active, highly stable, have a higher shelf life and can be reused repeatedly. Enzyme nanoparticles with 10-100 nm dimensions and exhibit unique physical, chemical and catalytic properties due to increased surface area. Nitrate reductase nanoparticles can be used as a biochemical tool to increase protein production and grain yield by promoting amino acids production in plants. The synthesised NaR NPs are used in the fabrication of enzyme-based nanosensor in the detection of nitrates in drinking water and serum samples.

Synthesis and Characterization of M + Co V 2 O 5 (M + = Li + , Na + , K + ) and Their Electrochemical Impedance Spectroscopic Studies

2017 ◽  
Vol 4 (11) ◽  
pp. 12291-12299
Author(s):  
Y.N. Vaidyanath ◽  
K.G. Ashamanjari ◽  
M. Mylarappa ◽  
M.S. Bhargava Ramu ◽  
K.R. Vishnu Mahesh ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Spectroscopic Studies ◽  
Synthesis And Characterization ◽  
Electrochemical Impedance Spectroscopic

scholarly journals Cu-Doped ZnO Nanoparticles for Non-Enzymatic Glucose Sensing

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 929
Author(s):  
Amira Mahmoud ◽  
Mosaab Echabaane ◽  
Karim Omri ◽  
Julien Boudon ◽  
Lucien Saviot ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Sol Gel ◽  
Chemical Properties ◽  
Differential Pulse ◽  
Glucose Sensing ◽  
Serum Samples ◽  
Experimental Conditions ◽  
Active Electrode ◽  
Transmission Electron

Copper-doped zinc oxide nanoparticles (NPs) CuxZn1−xO (x = 0, 0.01, 0.02, 0.03, and 0.04) were synthesized via a sol-gel process and used as an active electrode material to fabricate a non-enzymatic electrochemical sensor for the detection of glucose. Their structure, composition, and chemical properties were characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier-transform infrared (FTIR) and Raman spectroscopies, and zeta potential measurements. The electrochemical characterization of the sensors was studied using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV). Cu doping was shown to improve the electrocatalytic activity for the oxidation of glucose, which resulted from the accelerated electron transfer and greatly improved electrochemical conductivity. The experimental conditions for the detection of glucose were optimized: a linear dependence between the glucose concentration and current intensity was established in the range from 1 nM to 100 μM with a limit of detection of 0.7 nM. The proposed sensor exhibited high selectivity for glucose in the presence of various interfering species. The developed sensor was also successfully tested for the detection of glucose in human serum samples.

scholarly journals Optical, Photoelectrochemical and Impedance Studies on Poly 3,3’ Dibromo, 2,2’ Bithiophene: Effect of Bromine as a Substituent at C-3 Position in 2,2’ Bithiophene Molecules

2018 ◽  
Vol 10 (4) ◽  
pp. 83 ◽  
Author(s):  
Kasem K. Kasem ◽  
Houria Sadou ◽  
Anthony W. Jeffers
Keyword(s):  
Optical Band Gap ◽  
Electrochemical Impedance ◽  
Thiophene Ring ◽  
Spectroscopic Studies ◽  
Dielectric Constants ◽  
Blue Color ◽  
Oxide Electrode ◽  
Optical Studies ◽  
Red Color ◽  
Electrochemical Impedance Spectroscopic

Thin films of poly 3, 3’ dibromo, 2,2’ biththiophene (PDBrBTh) and poly 2,2’ biththiophene (PBTh) on fluorine doped tin oxide electrode (FTO) were subjected to optical, electrochemical and impedance studies in various aqueous electrolytes. Both polymers showed electrochromic properties, where the neutral film at -1.0 V vs Ag/AgCl shows red color, and the oxidized (doped) film at positive potentials shows blue color. Optical studies indicated that PDBrBTh showed a greater optical band gap than that of PBTh. Our studies also showed that the presence of bromine as a substituent at the C-3 position of the thiophene ring causes a greater ionization potential (IP) than PBTh. PDBrBTh also shows closer values of refractive index(n), and real (εr,) and imaginary (εi) dielectric constants to that of PBTh at photon energies between 2.0eV and 3.0 eV. At lower photon energy PDBrBTh showed lower optical conductivity than Br-free PBTh. Study also indicated that PDBrBTh is much less photoactive that PBTh. Electrochemical impedance spectroscopic studies (EIS) revealed that PDBrBTh possesses less relaxation time, and less double layer capacitance (Cdl) in dark and under illumination.

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