Investigation of lncRNAs JPX and LINC00641 Expression in the Fresh Frozen Tissue of Prostate Cancer Patients Compared to the Adjacent Non-Tumoral Prostate Tissue

This study was designed to identify and validate potential new biomarkers for prostate cancer and to distinguish patients with and without biochemical relapse. Prostate tissue samples analyzed by 2D-DIGE (two-dimensional difference in gel electrophoresis) and mass spectrometry (MS) revealed downregulation of secernin-1 (P< 0.044) in prostate cancer, while vinculin showed significant upregulation (P< 0.001). Secernin-1 overexpression in prostate tissue was validated using Western blot and immunohistochemistry while vinculin expression was validated using immunohistochemistry. These findings indicate that secernin-1 and vinculin are potential new tissue biomarkers for prostate cancer diagnosis and prognosis, respectively. For validation, protein levels in urine were also examined by Western blot analysis. Urinary vinculin levels in prostate cancer patients were significantly higher than in urine from nontumor patients (P= 0.006). Using multiple reaction monitoring-MS (MRM-MS) analysis, prostatic acid phosphatase (PAP) showed significant higher levels in the urine of prostate cancer patients compared to controls (P= 0.012), while galectin-3 showed significant lower levels in the urine of prostate cancer patients with biochemical relapse, compared to those without relapse (P= 0.017). Three proteins were successfully differentiated between patients with and without prostate cancer and patients with and without relapse by using MRM. Thus, this technique shows promise for implementation as a noninvasive clinical diagnostic technique.

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Abstract 160: Identifying differential methylation patterns of benign and tumor prostate tissue in African American and European American prostate cancer patients

10.1158/1538-7445.am2020-160 ◽

2020 ◽

Author(s):

Meytal B. Chernoff ◽

Kathryn Demanelis ◽

Marc Gillard ◽

Dayana Delgado ◽

Donald J. Vander Griend ◽

...

Keyword(s):

Prostate Cancer ◽

African American ◽

Cancer Patients ◽

Differential Methylation ◽

Prostate Tissue ◽

European American ◽

Methylation Patterns

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Immunohistochemical Study of IL-17 Expression in Prostate Tissue of a Prostate Cancer Patients

International Journal for Sciences and Technology ◽

10.12816/0017835 ◽

2015 ◽

Vol 10 (2) ◽

pp. 37-41

Author(s):

Hassan H. Zrage ◽

Zainab F. Ashoor ◽

Wasan A. Bakir

Keyword(s):

Prostate Cancer ◽

Cancer Patients ◽

Immunohistochemical Study ◽

Prostate Tissue

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Low stroma androgen receptor level in normal and tumor prostate tissue is related to poor outcome in prostate cancer patients

The Prostate ◽

10.1002/pros.20927 ◽

2009 ◽

Vol 69 (8) ◽

pp. 799-809 ◽

Cited By ~ 73

Author(s):

Pernilla Wikström ◽

Josip Marusic ◽

Pär Stattin ◽

Anders Bergh

Keyword(s):

Prostate Cancer ◽

Androgen Receptor ◽

Cancer Patients ◽

Prostate Tissue ◽

Receptor Level ◽

Poor Outcome

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N-Linked Glycosylation and Near-Infrared Spectroscopy in the Diagnosis of Prostate Cancer

International Journal of Molecular Sciences ◽

10.3390/ijms20071592 ◽

2019 ◽

Vol 20 (7) ◽

pp. 1592

Author(s):

Tijl Vermassen ◽

Sander De Bruyne ◽

Jonas Himpe ◽

Nicolaas Lumen ◽

Nico Callewaert ◽

...

Keyword(s):

Prostate Cancer ◽

Infrared Spectroscopy ◽

Near Infrared Spectroscopy ◽

Near Infrared ◽

Nir Spectroscopy ◽

Prostate Tissue ◽

Malignant Tissue ◽

Prostate Hyperplasia ◽

Fresh Frozen ◽

Tissue Specimens

Background: Performing a prostate biopsy is the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading. As little to no biochemical markers for prostate tissue exist, we explored the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis. Methods: Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained. The fresh-frozen tissue was dispersed and a tissue N-glycosylation profile was determined. Consequently, the formalin-fixed paraffin-embedded slides were analyzed using NIR spectroscopy. A comparison was made between the benign and malignant tissue, and between the various Gleason scores. Results: A difference was observed for the tissue of N-glycosylation between the benign and malignant tissue. These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001). These differences were also present between various Gleason scores. In addition, the NIR spectra revealed changes between the benign and malignant tissue in several regions. Moreover, spectral ranges of 1055–1065 nm and 1450–1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195). Conclusions: We have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores. These changes can be correlated to the changes observed in the NIR spectra. This could possibly further improve the histological assessment of PCa diagnosis, although further method validation is needed.

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Immunohistochemical Analysis of Prostein Expression in Archived Prostatic Core Biopsies from Prostate Cancer Patients in Western Kenya

10.21203/rs.3.rs-1132411/v1 ◽

2021 ◽

Author(s):

Omondi Swaya Tyrus ◽

Dedan Opondo ◽

David O. Atandi ◽

Benard Guyah ◽

Ng’wena Gideon Magak

Keyword(s):

Prostate Cancer ◽

Cancer Patients ◽

Core Biopsy ◽

Staining Pattern ◽

Immunohistochemical Analysis ◽

Composite Score ◽

Prostate Tissue ◽

Diagnostic Utility ◽

Western Kenya ◽

Low Sensitivity

Abstract Background Prostate cancer is the leading cause of cancer-associated mortality in men. Most of the current biomarkers for detection of the disease have low sensitivity and specificity. Prostein is a newly reported prostate cancer biomarkers whose diagnostic utility can help in early detection of the disease. Nonetheless, previous studies have utilized limited number of samples to evaluate its immunohistochemistry (IHC) and reports on the African population are not available. The current study aimed to determine the prostein expression in archived prostatic core biopsies from prostate cancer patients in Western Kenya. Materials and Methods This was a retrospective study conducted on malignant and benign prostatic tissue core biopsies of 106 patients who underwent prostate core biopsy at Jaramogi Oginga Odinga Teaching and Referral Hospital and division of urology at Synergy Clinics, Kisumu between January 2018 to May 2021. Immunohistochemical technique was performed on each of the 106 samples and on the following non-prostatic male control biopsies; Testis, Penis, Liver and Esophagus. Cellular location of prostein staining was evaluated at X40, X100 and X400 magnification using a light microscope and was classified as cytoplasmic or nucleocytoplasmic. Intensity of prostein expression was assessed for each core biopsy at similar magnification and graded according the immunohistochemistry composite score. Results The biopsies had been obtained from men whose mean (SE) age was 72.00±0.93 years. 95.3% (101) of the biopsies were malignant and 4.7% (5) were benign. Four non-prostatic male tissues were included. 97% of malignant and all the benign prostate tissue stained positive for prostein whereas the four non-prostatic male tissues were negative. Staining intensities were weak (24.5%), Moderate (17.0%), strong (55.7%) and non-stained (2.8%). The staining was highly immunolocalized within the cytoplasm (95.1% cases) as compared to nucleocytoplasmic (2.0% cases). The mean immunoreactivity composite score was 1.91±0.96 (0.0-3.14). Strongly stained sections had a punctate plasma membrane staining pattern clustered within the cytoplasm in a perinuclear location whereas the weakly stained sections had faint and punctate coarse brown cytoplasmic granular appearing. Conclusion Prostein is exclusively expressed in benign and malignant prostate tissue with a higher cytoplasmic granular staining pattern in the present population. These findings suggest that prostein diagnostic utility is applicable in the current study population and routine IHC diagnosis of prostate cancer may be recommended.

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