scholarly journals IN VITRO CALLUS INDUCTION OF SMILAX WIGHTII A.DC AN ENDEMIC MEDICINAL PLANT

2015 ◽  
Vol 2 (1) ◽  
pp. 97-101
Author(s):  
Nantha kumar R ◽  
Abdul kaffoor H ◽  
Arumugasamy K ◽  
Shalimol A ◽  
Asha devi V
Keyword(s):  
Medicinal Plant ◽  
Western Ghats ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
The Family ◽  
Half Strength ◽  
Endemic Medicinal Plant ◽  
The Western Ghats ◽  
Rooting Response

Smilax wightii A.DC is an endemic medicinal plant belongs to the family smilacaceae and distributed in Kodanadu, The Nilgiri Hills, The Western Ghats, Southern India. The callus was obtained at the concentration of 1.5+0.05 mg/I TDZ with NAA. Highest number of shoots was observed in 2.0+0.04 mg/l BAP + Kn and followed by 2.0+0.04mg/l. The multiplied shoots were harvested and used for rooting on half strength MS medium containing indole-3-butyric acid and naphthalene acetic acid within 45 days. The best rooting response was achieved on half-strength MS medium supplemented with 2.0 mg/l IBA. The well rooted plantlets were acclimatized and successfully transferred to natural condition, where 85% plantlets were survived

scholarly journals In Vitro Propagation of Digitalis trojana Ivanina., an Endemic Medicinal Plant of Turkey

2020 ◽  
Author(s):  
Nurşen Çördük ◽  
Cüneyt Aki
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
In Vitro Propagation ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Northwestern Turkey ◽  
Helen Of Troy ◽  
Endemic Medicinal Plant

Digitalis trojana Ivanina is a member of the Plantaginaceae family and known by its common name, Helen of Troy foxglove. It is perennial endemic to Çanakkale and Balıkesir, northwestern Turkey. In order to develop an efficient shoot regeneration protocol, the leaf explants of D. trojana were cultured on Murashige and Skoog (MS) medium containing 6-benzyl adenine (0.1, 0.5, 1.0, 3.0, 5.0 mg/L) and α-naphthalene acetic acid (0.1, 0.5, 1.0 mg/L), 3% (w/v) sucrose and 0.8% (w/v) agar. The highest number of regenerated shoots was obtained from leaf explants that were cultured on MS medium with 3.0 mg/L BA+0.1 mg/L NAA. Regenerated shoots were rooted on MS medium without plant growth regulators. Rooted plants (2–3 cm) were separately transferred to pots containing a mixture of peat and perlite (2:1 v/v) and acclimatized successfully in a growth chamber.

scholarly journals MICROPROPAGATION OF AN ENDANGERED AND ENDEMIC MEDICINAL PLANT CAYRATIA PEDATA VAR. GLABRA

2020 ◽  
pp. 191-196
Author(s):  
SHARMILA S ◽  
RAMYA EK ◽  
MOWNIKA S ◽  
DHIVYA SM
Keyword(s):  
Medicinal Plant ◽  
Callus Formation ◽  
Naphthalene Acetic Acid ◽  
Stem Explants ◽  
Root Induction ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Mass Multiplication ◽  
Endemic Medicinal Plant

Objective: The objective of the present study was to develop standardization protocol for the successful in vitro mass propagation of Cayratia pedata var. glabra through leaf and stem explants, since it is a rare, endangered, and endemic medicinal plant using biotechnological involvements and to conserve this endangered species. Methods: The application of biotechnological principles for the establishment of micropropagation under in vitro conditions has been studied by following the methods. The explants, namely, leaf and stem harvested from in vivo plants were thoroughly washed and properly sterilized with sterilients. The explants were transferred to Murashige and Skoog (MS) medium supplemented with growth regulators 6-benzyladenine (BAP) and naphthalene acetic acid (NAA) in the concentration range of 0.5–3.0 mg/l which were tested for callus induction and morphogenesis. The elongated shoots were transferred to MS medium supplemented with NAA at different concentrations for root induction. Results: The explants collected from the field (shola) were treated in different steriliants with various concentrations at different time for sterilization. Among the various combinations tried, the Teepol treatment for 10 min followed by bavistin 20 min, antibiotics, namely, ampicillin and rifampicin for 20 min, 70% alcohol for 30 s, and 0.12 % HgCl2 for 3 min was found to be effective. The explants were cultured in MS medium supplemented with various concentrations of BAP and NAA. The results noted that an increase in the concentration of BAP concomitantly reduced the frequency of callus formation. The maximum callusing frequency and more number of shoot formation was observed in the lower concentration of BAP (0.5 mg/l) in combination with NAA (0.2 mg/l). The callus obtained from all the above combinations was sub-cultured on MS medium with same combinations of BAP and NAA. The maximum frequency of root formation in leaf callus was 85% and 75% in stem callus and both were achieved on MS medium with NAA (1 mg/l) after 2 weeks. Conclusions: The current investigation provides a competent in vitro propagation method for C. pedata var. glabra which could be commercialized for developing identical plants with high-quality mass multiplication rate and for better conservation of the germplasm. Both the methods described here are well suited for the mass multiplication of this critically endangered and endemic climber species.

scholarly journals In vitro propagation of Boerhaavia diffusa L.: An important medicinal plant of family Nyctagimaceae

2019 ◽  
Vol 79 (01) ◽  
Author(s):  
Ashu Pandey ◽  
Oshin Verma ◽  
Suresh Chand
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
Liquid Medium ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Half Strength ◽  
Boerhaavia Diffusa ◽  
Important Medicinal Plant

Boerhaavia diffusa L., also known as santhi, or punarnava is an important medicinal plant, belonging to the family Nyctaginaceae. This species is said to be distributed throughout Malwa plateau in central India, as per ayurvedic literature, but due to extensive commercial exploitation, the species has become vulnerable. For callus induction, leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4- dichlorophenoxy acetic acid (2, 4-D 2.26 µM-9.04 µM) and N 6-benzyladenine (BA 1.11 µM-4.44 µM), either alone or in combinations. Calli formed within 10-12 days of culture, followed by shoots regeneration within 20-25 days. Direct organogenesis was achieved from nodal explants in MS media fortified with 2,4-D (2.26 µM-9.04 µM) along with BA (1.11 µM-4.44 µM) within 20 days. Multiple shooting was observed during subculture of in vitro regenerated shoots when 2,4-D was replaced with α-naphthalene acetic acid (NAA). Rooting was achieved in MS medium fortified with 2.85 µM IAA, within 7-10 days and also on half strength MS medium containing 2.85 µM Indole-3-acetic acid (IAA). For hardening, regenerated plants, with roots (3-4cm) were initially maintained on half-strength MS liquid medium (MS1/2) without growth regulators followed by quarter strength MS (MS1/4) liquid medium for 10 days. For acclimatization sterile mixture of soil, sand and manure (2:1:1) was used. Survival rate of regenerated plants was nearly 100%.

scholarly journals In vitro multiplication, micromorphological studies and ex vitro rooting of Hybanthus enneaspermus (L.) F. Muell. – a rare medicinal plant

2018 ◽  
Vol 77 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Nodal Segments ◽  
Ms Medium ◽  
Ex Vitro ◽  
Culture Bottle ◽  
Hybanthus Enneaspermus ◽  
Half Strength

AbstractHybanthus enneaspermusis a rare medicinal plant. We defined a protocol for micropropagation,ex vitrorooting of cloned shoots and their acclimatization. Surface-sterilized nodal segments were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin). Medium supplemented with 1.5 mg L−1BAP was found optimum for shoot induction from the explants and 6.4±0.69 shoots were regenerated from each node with 97% response. Shoots were further proliferated maximally (228±10.3 shoots per culture bottle with 7.5±0.43 cm length) on MS medium augmented with 1.0 mg L−1each of BAP and Kin within 4–5 weeks. The shoots were rootedin vitroon half strength MS medium containing 2.0 mg L−1indole-3 butyric acid (IBA). The cloned shoots were pulse-treated with 300 mg L–1 of IBA and cultured on soilrite® in a greenhouse. About 96% of the IBA-pulsed shoots rootedex vitroin soilrite®, each shoot producing 12.5±0.54 roots with 5.1±0.62 cm length. Theex vitrorooted plantlets showed a better rate of survival (92%) in a field study thanin vitrorooted plantlets (86%). A comparative foliar micromorphological study ofH. enneaspermuswas conducted to understand the micromorphological changes during plant developmental processes fromin vitrotoin vivoconditions in terms of variations in stomata, vein structures and spacing, and trichomes. This is the first report onex vitrorooting inH. enneaspermusand the protocol can be exploited for conservation and large-scale propagation of this rare and medicinally important plant.

scholarly journals Micropropagation of Achillea millefolium L. on half-strength ms medium and direct rooting and acclimatization of microshoots in hydroponic culture

2015 ◽  
pp. 99-112
Author(s):  
Marija Markovic ◽  
Dragana Skocajic ◽  
Mihailo Grbic ◽  
Matilda Djukic ◽  
Dragica Obratov-Petkovic ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Nutrient Solution ◽  
Ph Value ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Efficient Production ◽  
Ms Medium ◽  
Ex Vitro ◽  
Half Strength

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution.

scholarly journals Improved micropropagation and foliar micromorphological studies in Turnera ulmifolia L. – An important medicinal plant

2018 ◽  
Vol 30 (2) ◽  
pp. 283-294 ◽  
Author(s):  
Mani Manokari ◽  
Mahipal S. Shekhawat
Keyword(s):  
Shoot Proliferation ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Half Strength ◽  
Turnera Ulmifolia ◽  
Semi Solid ◽  
Number Of Shoots

Abstract The present study reports an efficient in vitro propagation system for Turnera ulmifolia using nodal segments as explants. Turnera ulmifolia (Passifloraceae) is an important garden plant with multipotent medicinal values. Effective shoot proliferation was achieved on agar gelled MS medium (Murashige and Skoog, 1962). The maximum number of shoots (8.3 ± 0.57) per initial explant was obtained on MS medium supplemented with 8.88 mM of 6-benzylaminopurine (BAP) and 0.54 mM of α-naphthalene acetic acid (NAA). The highest number of shoots (59.5 ± 2.10) proliferated on semi-solid MS medium (with agar) augmented with 2.22 mM of BAP and 2.32 mM of kinetin (Kin) along with 0.54 mM of NAA. Longer (4-5 cm) and healthy shoots were rooted (12.0 ± 0.10 roots per shoot) on half-strength MS medium fortified with 9.84 mM of indole-3 butyric acid (IBA). The in vitro regenerated plantlets were hardened in the greenhouse and transferred to the field. Significant developmental changes were observed in the foliar micromorphology of in vitro raised plantlets when these were transferred to the field. The stomatal index was gradually reduced (26.72 to 21.25) in the leaves from in vitro to field environments. But, vein-islets and veinlet terminations (13.4 and 7.6) were increased (39.7 and 18.4) respectively from in vitro to in vivo grown plants. Simple, unicellular, less frequent and underdeveloped trichomes were observed with the leaves of in vitro plants but fully developed trichomes recorded in the field transferred plants. The study could help in understanding the response and adaptation of tissue culture raised plantlets towards changed environmental conditions.

scholarly journals In vitro Propagation of Lychnophora pinaster (Asteraceae): A Threatened Endemic Medicinal Plant

HortScience ◽  
2007 ◽  
Vol 42 (7) ◽  
pp. 1665-1669 ◽  
Author(s):  
Ana V. de Souza ◽  
José E.B.P. Pinto ◽  
Suzan K.V. Bertolucci ◽  
Ricardo M. Corrêa ◽  
Larissa C. do B. Costa ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
In Vitro Propagation ◽  
Shoot Elongation ◽  
Great Difficulty ◽  
Naphthalene Acetic Acid ◽  
Alcoholic Extract ◽  
Shoot Induction ◽  
Plantlet Growth ◽  
Endemic Medicinal Plant

Lychnophora pinaster, known as arnica, is a medicinal plant of the Cerrado ecosystem in Brazil. It is widely used in the form of alcoholic extract for its anti-inflammatory and anesthetic and healing effects on sprains, bruises, and inflammation. Owing to the great difficulty of propagation, it is listed by the Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis in the category of plants vulnerable to extinction. Micropropagation offers a solution to this problem by allowing the preservation and expansion of germplasm. The objective of this research was to establish a protocol for in vitro propagation of arnica. The best medium for germination of arnica embryos and plantlet growth was a quarter strength semisolid Murashige and Skoog medium (MS/4) containing 0.75% (w/v) sucrose. For shoot induction, the best results were obtained on MS/4 with 2.76 μm of benzylaminopurine. Maximum shoot elongation before rooting occurred in the presence of 8.67 μm of gibberellic acid for 19 d. Microshoots were successfully rooted in the presence of 10.7 μm of naphthalene acetic acid for 15 d. After rooted plantlets were acclimatized in a greenhouse for 20 d, the survival rate was 100% when planted in a soil from the area of occurrence of the species, whereas 0% survived when planted in Plantmax.

scholarly journals Micropropagation of traditional medicinal plant Ceropegia juncea

2018 ◽  
Vol 7 (2) ◽  
pp. 1992 ◽  
Author(s):  
Binish T.
Keyword(s):  
Medicinal Plant ◽  
Plant Extract ◽  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Ms Medium ◽  
Liver Disorders ◽  
Tribal People ◽  
Medicinal Properties ◽  
Endemic Medicinal Plant

Ceropegia species which possess wide medicinal properties are being used in different traditional medicinal systems that are used by tribal people for curing different ailments. Ceropegia juncea was reported to be the source of ‘Soma’, a plant drug of the Ayurvedic system of medicine. The plant extract is used for the treatment of anti-inflammatory, analgesic, antiulcer activities, liver disorders, hypotension, ulcerative condition and fever. It is also used as typical anesthetic agent. The present study was conducted to establish a protocol for in- vitro propagation of an endemic medicinal plant Ceropegia juncea maximum shoot proliferation better shoots with a sprouting frequency of 86% and with an average of 8.28 ±1.11 shoots /explants and attained a length of 5.37±0.74 cm was achieved on Murashige and Skoog’s, 1962 (MS) medium supplemented with 6-benzylaminopurine (BAP) 1.5 mg/L + NAA 1.0mg/L and highest rooting of in vitro derived shoots was achieved on half MS with IBA 0.75mg/L.

scholarly journals In vitro propagation of Nanorrhinum ramosissimum (Wall.) Betsche: A traditionally important medicinal plant

2021 ◽  
Vol 48 (3) ◽  
Author(s):  
Jyotsna Sharma ◽  
◽  
Anuja Koul ◽  
Savita Sharma ◽  
Raju Shankarayan ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Shoot Organogenesis ◽  
Germplasm Conservation ◽  
Ms Medium ◽  
In Vitro Plantlets ◽  
Half Strength ◽  
Shoot Tip Explants ◽  
Indole 3 Acetic Acid ◽  
Important Medicinal Plant

An efficient micropropagation protocol facilitates successful conservation and improvement of Nanorrhinum ramosissimum (Wall.) Betsche by biotechnological means. Shoot tip explants exhibited optimal organogenic response when inoculated on half-strength(1/2) Murashige and Skoog (MS) medium supplemented with kinetin (KN) and indole-3-acetic acid (IAA) (0.5 mg/L each). Shoot organogenesis was further enhanced when the multiplication medium was fortified with dextrose (1%) (2.6 shoots/explant; 7.9 cm shoot length). The regenerated shoots formed roots; however, the best rooting frequency (87%) was achieved on half-strength MS medium containing only IAA (0.5 mg/L). Four-week-old in vitro plantlets were acclimatized with 95% survival under greenhouse conditions. The regeneration protocol developed in this study can be utilized for germplasm conservation of this elite traditional medicinal plant.

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