Biochemical markers of oxidative stress in maize seedlings exposed to rose-grass aphids

2021 ◽  
Vol 53 (1) ◽  
pp. 23-34
Author(s):  
I. Łukasik ◽  
S. Goławska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Anion ◽  
Biochemical Markers ◽  
Carbonyl Groups ◽  
Maize Seedlings ◽  
Aphid Infestation ◽  
Markers Of Oxidative Stress ◽  
Aphid Feeding ◽  
Superoxide Anion Radicals

We studied the influence of rose-grass aphid (Metopolophium dirhodum L.) infestation on the biochemical markers of oxidative stress in seedlings of two maize (Zea mays L.) varieties (relatively resistant Ambrozja and resistant Plomyk). We compared the generation of superoxide anion radicals O2-, level of hydrogen peroxide (H2O2), lipid peroxidation products (MDA) as well as markers of protein damage (protein-bound carbonyl groups). The studied parameters were measured at 24, 48, 72 and 96 h post-initial aphid infestation compared to the non-infested control seedlings. Our studies indicated that the rose-grass aphid feeding evoked oxidative stress in the maize seedlings. Investigated Z. mays cultivars exhibited excessive generation of superoxide anion radicals in response to insect treatments. Rose-grass aphid feeding increased the H2O2 level in maize tissues with similar levels observed at most periods post-infestation with M. dirhodum, also increased lipid peroxidation products with the maximal levels at 48 and 72 h for Ambrozja and 48, 72 and 96 h post-infestation for Plomyk varieties. Further at 48 and 72 h post-initial aphid infestation, there was an increase in protein bound carbonyl groups content in the maize seedlings after infestation with aphids.

scholarly journals Transdermal nitroglycerin therapy is not associated with biochemical markers of oxidative stress

1998 ◽  
Vol 31 ◽  
pp. 395
Author(s):  
S.D. Milone ◽  
C.R. Pace-Asciak ◽  
E.R. Azevedo ◽  
G.E. Newton ◽  
J.D. Parker
Keyword(s):  
Oxidative Stress ◽  
Biochemical Markers ◽  
Markers Of Oxidative Stress

Lipid Peroxidation, Protein Oxidation, Gelatinases, and Their Inhibitors in a Group of Adults with Obesity

2019 ◽  
Vol 51 (06) ◽  
pp. 389-395 ◽  
Author(s):  
Gregorio Caimi ◽  
Baldassare Canino ◽  
Maria Montana ◽  
Caterina Urso ◽  
Vincenzo Calandrino ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Protein Oxidation ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
The Body ◽  
Control Group ◽  
The Matrix ◽  
Markers Of Oxidative Stress ◽  
Obese Subjects

AbstractThe association between obesity and cardiovascular diseases has a multifactorial pathogenesis, including the synthesis of inflammatory molecules, the increase in oxidative stress and the dysregulation of the matrix metalloprotease (MMP) concentration and activity. In a group of adults with obesity, divided in 2 subgroups according to the body mass index (BMI), we examined lipid peroxidation, expressed as thiobarbituric acid-reactive substances (TBARS), protein oxidation, expressed as protein carbonyl groups (PCs), plasma gelatinases (MMP-2 and MMP-9), and their tissue inhibitors (TIMP-1 and TIMP-2). In the whole group, as well as in the 2 subgroups (with BMI 30–35 or BMI>35) of obese subjects, we observed an increase in TBARS, PCs, MMP-2, and MMP-9, and also TIMP-1 and TIMP-2 in comparison with the control group. A positive correlation between TBARS and PCs emerged in obese subjects and persisted after dividing obese subjects according to BMI. The correlation between MMP-2 and TIMP-2 was not statistically significant, while a significant correlation was present between MMP-9 and TIMP-1. The correlations between the markers of oxidative stress (TBARS and PCs) and those of the MMP/TIMP profile indicated a more marked influence of protein oxidation on MMPs and TIMPs in comparison with TBARS. The innovative aspect of our study was the simultaneous evaluation of oxidative stress markers and MMP/TIMP profile in adult obese subjects. We observed significant alterations and correlations that may negatively influence the clinical course of the disease.

Study of biochemical markers of oxidative stress in smokers versus nonsmokers with mixed anxious and depressive disorder

2019 ◽  
Author(s):  
Letitia Trofor ◽  
Cristinel Stefanescu ◽  
Mona Cioroiu ◽  
Ioana Buculei ◽  
Andrei Cernomaz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Depressive Disorder ◽  
Biochemical Markers ◽  
Markers Of Oxidative Stress

scholarly journals The study on the antioxidant activity of polysaccharides isolated from Polygonatum odoratum (Mill.) Druce

2020 ◽  
Vol 21 (47) ◽  
pp. 12-18
Author(s):  
Wu Lan Gerile ◽  
Naranchimeg Dorjpalam ◽  
Wanyuan Gui ◽  
Liang Xu ◽  
Jinglin Liu
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Anion ◽  
Functional Food ◽  
Anion Radical ◽  
Oxygen Free Radicals ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Antioxidant Agents ◽  
Radical Scavenging Ability ◽  
Superoxide Anion Radicals

The polysaccharides isolated from Polygonatum odoratum (Mill.) Druce (POPs) by water extraction, after precipitation with ethanol were purified through deproteinization, decolorization, dialysis, and column chromatography leading to a purified polysaccharide (POPs-I) content of 90.7 %. The scavenging of oxygen free radicals and inhibition of lipid peroxidation (LPO) by POPs-I were analyzed using a colorimetric method. The results showed that the hydroxyl radical scavenging ability of the polysaccharides was weaker than that of benzoic acid, but stronger than those of ascorbic acid and mannitol, and that the superoxide anion radical scavenging ability was inferior to those of all three. When the concentration was higher than 1.0 mg/mL, the POPs-I could inhibit LPO by superoxide anion radicals to a certain degree. Therefore, this work suggests that POPs-I are potential antioxidant agents in medicine and functional food.

scholarly journals Influence of feeding thermally peroxidized soybean oil on growth performance, digestibility, gut integrity, and oxidative stress in nursery pigs

2020 ◽  
Vol 98 (2) ◽  
Author(s):  
Brian J Kerr ◽  
Stephanie C Lindblom ◽  
Martin F Overholt
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Soybean Oil ◽  
Growth Performance ◽  
Nursery Pigs ◽  
Lipid Peroxidation Products ◽  
Thermally Processed ◽  
Markers Of Oxidative Stress ◽  
Effect Of Feeding ◽  
Lipid Digestibility

Abstract The objectives of the current experiments were to evaluate the effect of feeding soybean oil (SO) with different levels of peroxidation on lipid, N, and GE digestibility, gut integrity, oxidative stress, and growth performance in nursery pigs. Treatments consisted diets containing 10% fresh SO (22.5 °C) or thermally processed SO (45 °C for 288 h, 90 °C for 72 h, or 180 °C for 6 h), each with an air infusion of 15 L/min, with postprocessing peroxide values of 7.6, 11.5, 19.1, and 13.4 mEq/kg and p-anisidine values of 1.92, 6.29, 149, and 159, for the 22.5 °C, 45 °C, 90 °C and 180 °C processed SO, respectively. In experiment 1, 64 barrows (7.1 ± 0.9 kg initial BW) were randomly allotted into 2 rooms of 32 pens and individually fed their experimental diets for 21 d, with a fresh fecal sample collected on day 20 for determination of GE and lipid digestibility. In experiment 2, 56 barrows (BW 9.16 ± 1.56 kg) were placed into individual metabolism crates for assessment of GE, lipid, and N digestibility and N retention. Urinary lactulose to mannitol ratio was assessed to evaluate in vivo small intestinal integrity, and urine and plasma were collected to analyze for markers of oxidative stress. Pigs were subsequently euthanized to obtain liver weights and analyze the liver for markers of oxidative stress. In experiment 1, pigs fed the SO thermally processed at 90 °C had reduced ADG (P = 0.01) and ADFI (P = 0.04) compared to pigs fed the other SO treatment groups, with no differences noted among pigs fed the 22.5 °C, 45 °C, and 180 °C SO treatments. No effects of feeding thermally processing SO on dietary GE or lipid digestibility (P > 0.10) were noted in either experiment. In experiment 2, there was no dietary effect of feeding peroxidized SO on the DE:ME ratio, N digestibility, or N retained as a percent of N digested, on the urinary ratio of lactulose to mannitol, on serum, urinary, or liver thiobarbituric acid reactive substances, on plasma protein carbonyls, or on urinary or liver 8-OH-2dG (P > 0.10). In experiment 2, pigs fed the SO thermally processed at 90 °C had the greatest isoprostane concentrations in the serum (P ≤ 0.01) and urine (P ≤ 0.05) compared to pigs fed the unprocessed SO. These results indicate that the change in fatty acid composition and/or the presence of lipid peroxidation products in peroxidized SO may reduce ADG and ADFI in nursery pigs, but appears to have no impact on GE, lipid, or N digestibility, or gut permeability. These data suggest that the presence of lipid peroxidation products may affect certain markers of oxidative stress.

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