Biochemical Markers of Free Radical Oxidation and Lipid Exchange in Rats with Obesity, Iodine Defficiency and Obesity in Combination with Iodine Defficiency

The purpose of the study was the content of lipid and protein peroxidation products, lipid spectrum parameters and the level of aminotransferases in obese, iodine deficient and obese rats in combination with iodine deficiency. Materials and methods. The study was performed on 45 white nonlinear rats weighing 120-180 g, which were divided into three experimental groups: obese rats (1st experimental group, n = 15), iodine-deficient animals (2nd experimental group), obese animals in combined with iodine deficiency (3rd experimental group, n = 15). The control group consisted of 15 intact rats. The content of products of lipid peroxidation and oxidative modification of proteins was determined in the blood and liver tissue of rats. Blood lipid spectrum was assessed by serum levels of triacylglycerols, total cholesterol, high-density lipoproteins and low-density lipoproteins, followed by calculation of the atherogenic factor. The activity of aspartate aminotransferase and alanine aminotransferase was determined in the blood. Results and discussion. It was found that in the liver tissue of rats and blood of experimental groups the content of lipid hydroperoxides and active products that react with thiobarbituric acid increases, which indicates the activation of lipoperoxidation processes. A variety of changes in protein peroxidation in both blood serum and liver tissue of animals of experimental groups was revealed. Regarding the lipid spectrum, the most pronounced differences in the indicators in relation to the control were found in obese animals in combination with iodine deficiency. In this group of animals, cholesterol was increased by 65% in reference to control, triacylglycerol content increased by 52%, low-density lipoprotein exceeded control by 60%, and high-density lipoprotein decreased by 61% in reference to control. The highest activity of aspartate aminotransferase was found in the group of animals with iodine deficiency, and alanine aminotransferase – in the group of obese animals. Conclusion. In the blood and liver tissue of rats with obesity, iodine deficiency and obesity in combination with iodine deficiency increases the content of products of free radical oxidation. The content of cholesterol, triacylglycerols, low-density lipoproteins increases in the blood, the content of high-density lipoproteins decreases, the activity of aspartate aminotransferase and alanine aminotransferase increases. The most pronounced differences in the indicators in reference to the control were found in obese animals in combination with iodine deficiency

Download Full-text

Dynamics of blood lipid spectrum indicators in children with juvenile idiopathic arthritis taking into account basic therapy

The Journal of V. N. Karazin Kharkiv National University, Series "Medicine" ◽

10.26565/2313-6693-2021-42-02 ◽

2021 ◽

Keyword(s):

Juvenile Idiopathic Arthritis ◽

Total Cholesterol ◽

Blood Lipid ◽

Pathological Process ◽

High Density ◽

High Density Lipoproteins ◽

Low Density ◽

Basic Therapy ◽

One Year ◽

Lipid Spectrum

Summary. In patients with rheumatoid arthritis, the risk of cardiovascular disease associated with atherosclerosis, is significantly increased compared to the general population. Studies of a cohort of children and young adults indicated that the atherosclerotic process usually begins long before its clinical manifestation, and the degree of vascular changes is associated with both the number of risk factors and their severity. The use of basic drugs in rheumatic patients contributed to a decrease in cardiovascular mortality, mainly due to the elimination of the activity of the process, which itself is an inducer of the onset and progression of atherosclerosis. The aim of the study was to establish the nature of changes in the blood lipid spectrum in children with juvenile idiopathic arthritis under the influence of basic therapy. Object and methods. We examined 85 children (8–18 years old) with juvenile idiopathic arthritis and oligo- (61.5 %) and polyarticular (38.5 %) variants of the disease, twice, with an interval of one year. The parameters of the blood lipid spectrum were determined by the level of: total cholesterol, triglycerides, high density lipoproteins, atherogenic coefficient by the enzymatic method on an automatic biochemical analyzer-photometer A25, low lipoproteins and very low density cholesterol by calculation method. Research results. Analysis of the lipid profile in the group as a whole showed that patients with juvenile idiopathic arthritis had significantly higher total cholesterol values (p < 0.05), a tendency to an increase in the level of triglycerides, low lipoproteins and very low density cholesterol, which led to an increase in the atherogenic coefficient level. When analyzing the lipid spectrum of blood after a year, a tendency towards a decrease in the level of atherogenic and an increase in antiatherogenic fractions of the lipid spectrum with normalization of atherogenic coefficient was noted. In the group of patients receiving monotherapy with methotrexate, no significant changes in the lipid spectrum of blood were observed after one year. Against the background of combined basic therapy, the blood lipid spectrum normalizes, namely, a decrease in total cholesterol, an increase in high density lipoproteins of cholesterol, a decrease in the level of low density cholesterol (p < 0.05), triglycerides and very low density cholesterol and, as a consequence, significant decrease in atherogenic coefficient (p < 0.01). Conclusions. The dynamics of changes in blood lipid spectrum indicators in children with juvenile idiopathic arthritis confirms the need to use combined basic therapy not only to control the activity of main pathological process, but also to prevent the development of atherogenic dyslipoproteinemias.

Download Full-text