scholarly journals Western Blot Analysis of the IgG-Antibody Response to Acid- Glycine-Extracted Antigens from Campylobacter fetus subsp. fetus and C. jejuni in Naturally Infected Sheep

2007 ◽  
Vol 76 (2) ◽  
pp. 245-251 ◽  
Author(s):  
K. Gürtürk ◽  
I. H. Ekin ◽  
A. Arslan
Keyword(s):  
Antibody Response ◽  
Western Blot ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Serological Tests ◽  
Recent Infection ◽  
Healthy Sheep ◽  
Apparently Healthy

IgG-antibody response in aborting sheep and in apparently healthy sheep in a flock against acidglycine- extracted antigens from three strains for each C. fetus subsp. fetus and C. jejuni were analysed by Western blot. One strain of C. fetus subsp. fetus was isolated from aborting sheep. Western blot analysis of the sera revealed the presence of IgG antibody binding to the common antigens including proteins with the Mw of 63 kDa and 54 kDa in extracts from both C. fetus subsp. fetus and C. jejuni strains. In addition, IgG antibodies in sera from aborting sheep reacted more strongly with the antigens from C. fetus subsp. fetus strains with Mw of approximately 100, 95 and 86.5 kDa than those of apparently healthy sheep. The binding profile of the antibodies with these antigens appeared to be unique for each C. fetus subsp. fetus strain. On the other hand, IgG antibodies only in sera from aborting sheep recognized strongly the antigens of each C. fetus subsp. fetus strain at the Mw ranged from approximately 26 to 22 kDa. However, the antigenic components between 26 and 22 kDa were not detectable in coomassie blue stained gel and thought to have non-protein nature. These low molecular weight antigens of C. fetus subsp. fetus may be related to a recent infection in aborting sheep. These observations indicate that such speciesspecific antigens or conjugated protein antigens could be used for improving the specificity of the serological tests to detect C. fetus antibodies in sheep sera, and may be the candidates for subunit vaccines against ovine abortion.

scholarly journals The antibody response in Legionnaires' disease

1979 ◽  
Vol 83 (2) ◽  
pp. 377-381 ◽  
Author(s):  
J. Nagington ◽  
T. G. Wreghitt ◽  
J. O'H. Tobin ◽  
A. D. Macrae
Keyword(s):  
Antibody Response ◽  
Serological Test ◽  
Igg Antibodies ◽  
Immunofluorescence Technique ◽  
Igg Antibody ◽  
Recent Infection ◽  
Igm Antibody ◽  
Serotype 1 ◽  
Indirect Immunofluorescence Technique ◽  
Legionnaires Disease

summaryFrom 22 patients with Legionnaires' disease, 86 sera were examined for specific serotype 1 IgM and IgG antibodies by the indirect immunofluorescence technique.No antibody was detectable until 8 days or more from the onset of symptoms. When produced the amount was widely variable and remained detectable for periods from less than 34 days to more than 1 year.Initially IgM antibody predominated, ten patients produced only IgM in the first 21 days, six produced only IgM in the first 28 days and three did not produce IgG at any time. One patient, and possibly a second, produced only IgG antibody.Since IgM antibody was still present in one patient after a year it is important not to accept the presence of this as evidence of very recent infection.It is advisable that any type of serological test for L. pneumophila infection should detect the production of both IgM and IgG antibodies.

Western blot analysis of antibody response to pneumococcal protein antigens in a murine model of pneumonia.

1997 ◽  
Vol 4 (6) ◽  
pp. 778-782
Author(s):  
H Mouneimne ◽  
M Juvin ◽  
J L Beretti ◽  
E Azoulay-Dupuis ◽  
E Vallee ◽  
...  
Keyword(s):  
Antibody Response ◽  
Western Blot ◽  
Murine Model ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Protein Antigens

scholarly journals Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep

1994 ◽  
Vol 113 (2) ◽  
pp. 377-385 ◽  
Author(s):  
P. Chand ◽  
R. P. Kitching ◽  
D. N. Black
Keyword(s):  
Western Blot ◽  
Specific Antibody ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Viral Infections ◽  
Antibody Responses ◽  
Serological Tests ◽  
Virus Specific Antibody ◽  
Firm Basis ◽  
Contagious Pustular Dermatitis

SUMMARYThis paper reports the development and evaluation of serological tests for the differentiation of antibodies in animals infected with capripox and parapox viruses. Agar-gel immunodiffusion tests using sera from sheep with naturally-acquired infections and from sheep experimentally inoculated with orf or capripox viruses showed cross reactions. Virus-specific antibody responses to structural proteins of the viruses were analysed by Western-blot analysis. This analysis readily differentiated the infections as either capripox or contagious pustular dermatitis. The antibody responses to the 32 kDa and 26 kDa proteins of capripox virus provided a firm basis for differention.

scholarly journals Comparison of Western Blot Technique with Microscopy and Culture for Diagnosis of Tuberculosis

2016 ◽  
Vol 1 (1) ◽  
pp. 21-24
Author(s):  
Md. Jamal Uddin Gazi ◽  
Md. Ruhul Amin Miah ◽  
Sharmeen Ahmed ◽  
Abu Naser Ibne Sattar
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Antibody Response ◽  
Western Blot ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Polyacrylamide Gel ◽  
Serum Samples ◽  
Bacteriological Diagnosis ◽  
Blot Technique ◽  
Study Population

Diagnosis of tuberculosis is usually done by microscopy for AFB and other tests. But each test has different limitation. This study was carried out to compare western blot technique with microscopy and culture for diagnosis of tuberculosis. Sputum and other samples were collected from 112 TB patients for bacteriological diagnosis by microscopy and culture for AFB. Serum samples were collected from 112 TB patients and 50 control subjects for detection of antibody response by western blot. For western blot analysis, Mycobacterium tuberculosis sonicate antigen extract was fractionated by electrophoresis on  polyacrylamide gel.  Western blot analysis revealed four polypeptides against which antibody response of study population were observed. The sensitivity of western blot was 73.21% which was higher than that of microscopy (63.39%) and culture (57.14%) for diagnosis of tuberculosis. The specificity of western blot was 92%.Bangladesh J Med Microbiol 2007; 01 (01):21-24

Radiosensitivity of glioma to Gamma Knife treatment enhanced in vitro and in vivo by RNA interfering Ku70 that is mediated by a recombinant adenovirus

2010 ◽  
Vol 113 (Special_Supplement) ◽  
pp. 228-235 ◽  
Author(s):  
Qiang Jia ◽  
Yanhe Li ◽  
Desheng Xu ◽  
Zhenjiang Li ◽  
Zhiyuan Zhang ◽  
...  
Keyword(s):  
Western Blot ◽  
Gamma Knife ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
C6 Cells

Object The authors sought to evaluate modification of the radiation response of C6 glioma cells in vitro and in vivo by inhibiting the expression of Ku70. To do so they investigated the effect of gene transfer involving a recombinant replication-defective adenovirus containing Ku70 short hairpin RNA (Ad-Ku70shRNA) combined with Gamma Knife treatment (GKT). Methods First, Ad-Ku70shRNA was transfected into C6 glioma cells and the expression of Ku70 was measured using Western blot analysis. In vitro, phenotypical changes in C6 cells, including proliferation, cell cycle modification, invasion ability, and apoptosis were evaluated using the MTT (3′(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide) assay, Western blot analysis, and cell flow cytometry. In vivo, parental C6 cells transfected with Ad-Ku70shRNA were implanted stereotactically into the right caudate nucleus in Sprague-Dawley rats. After GKS, apoptosis was analyzed using the TUNEL (terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling) method. The inhibitory effects on growth and invasion that were induced by expression of proliferating cell nuclear antigen and matrix metalloproteinase–9 were determined using immunohistochemical analyses. Results The expression of Ku70 was clearly inhibited in C6 cells after transfection with Ad-Ku70shRNA. In vitro following transfection, the C6 cells showed improved responses to GKT, including suppression of proliferation and invasion as well as an increased apoptosis index. In vivo following transfection of Ad-Ku70shRNA, the therapeutic efficacy of GKT in rats with C6 gliomas was greatly enhanced and survival times in these animals were prolonged. Conclusions Our data support the potential for downregulation of Ku70 expression in enhancing the radiosensitivity of gliomas. The findings of our study indicate that targeted gene therapy–mediated inactivation of Ku70 may represent a promising strategy in improving the radioresponsiveness of gliomas to GKT.

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