scholarly journals Immunogenicity and Safety of La Sota Strain of Newcastle Disease Virus Administered to Newly Hatched Chicks by Nebulization

2009 ◽  
Vol 78 (1) ◽  
pp. 137-144 ◽  
Author(s):  
Hrvoje Mazija ◽  
Stanislav Čajavec ◽  
Estella Prukner-Radovčić ◽  
Neda Ergotić ◽  
Irena Ciglar-Grozdanić ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Specific Pathogen Free ◽  
Delivery Method ◽  
Closed Chamber ◽  
Specific Pathogen ◽  
Backyard Flocks ◽  
The Given ◽  
Commercial Chickens

The objective of four trials performed on specific-pathogen-free and commercial chickens, either of light or heavy hybrids, was to evaluate the new vaccine delivery method to newly hatched chickens using commercial La Sota vaccine. The vaccine was given by means of nebulization using an ultrasonic device producing homologous aerosol of particles ranging 3–5 microns in diameter. Chickens were exposed to the La Sota vaccine for 30, 60 or 300 s in a closed chamber of the device, thus enabling constant particle size during vaccination. No adverse reaction to the given vaccine was recorded, and the immunity, developed no later than 7 days after vaccination, lasted for at least 49 days which was confirmed by challenge infection using Herts 33 strain of Newcastle disease virus. Maternal antibodies did not influence the development of immunity. Regarding the mode of vaccination, the described method is suitable for the control of Newcastle disease in both big poultry enterprises as well as small backyard flocks when newly hatched chickens are supplied from local hatcheries.

scholarly journals Newcastle disease virus induces testicular damage and disrupts steroidogenesis in specific pathogen free roosters

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Zaib Ur Rehman ◽  
Shanhui Ren ◽  
Bin Yang ◽  
Xiaofeng Yang ◽  
Salman Latif Butt ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Specific Pathogen Free ◽  
Testicular Damage ◽  
Specific Pathogen

scholarly journals Quantitative evaluation of viral interference among Egyptian isolates of highly pathogenic avian influenza viruses (H5N1 and H5N8) with the lentogenic and velogenic Newcastle disease virus genotype VII in specific pathogen-free embryonated chicken eggs model

2019 ◽  
Vol 12 (11) ◽  
pp. 1833-1839
Author(s):  
Mohamed A. Soliman ◽  
Ahmed A. Nour ◽  
Ahmed M. Erfan
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Highly Pathogenic Avian Influenza ◽  
Specific Pathogen Free ◽  
Highly Pathogenic ◽  
Pathogenic Avian Influenza ◽  
Specific Pathogen ◽  
Embryonated Chicken

Background and Aim: Mixed infections of the highly pathogenic avian influenza virus (HPAIV) and Newcastle disease virus (NDV) are considered the most distressing problem of the poultry industry. The problem arises due to the influence of a hidden virus on the replication of another suspected virus. Consequently, misdiagnosis of the real cause of disease may become a source of infection for other healthy stock by transmission and dissemination of the hidden virus. This study aimed to determine the impact of HPAIV and NDV on each other in a specific pathogen-free embryonated chicken egg (SPF-ECE) model. Materials and Methods: HPAIVs (H5N1 and H5N8) and NDVs [avirulent NDV [avNDV] and velogenic NDV [vNDV]) were inoculated into the allantois cavity of SPF-ECE with graded titers (2, 3, and 4 log10 EID50) at 24 and 48 h of incubation, followed by the collection of allantoic fluid. A quantitative reverse transcription real-time polymerase chain reaction was used to determine the viral RNA copies of both viruses. Results: Obvious interference was reported on the growth of NDVs when co-inoculated with AIVs. NDV RNA titers reduction ranged from <3 to 5 log10 to complete suppression, but slight interference with the growth of AIVs occurred. H5N1 RNA titers showed <1-2 log10 reduction when co-inoculated with vNDV compared with the H5N1 control. The interference impact of H5N8 was more powerful than that of H5N1, while vNDV showed more resistance for interference than the avNDV strain. On the other hand, interference of AIVs was not observed except when vNDV was inoculated before H5N1. The interfering impact was increased after 48 h of inoculation, whereas no titer of avNDV was detectable. Conclusion: AIV strains had a powerful effect on NDV growth, regardless of which infection occurred first.

scholarly journals Ultrastructural Changes of the Tracheal Epithelium after Vaccination of Day-Old Chickens with the La Sota Strain of Newcastle Disease Virus

2005 ◽  
Vol 42 (5) ◽  
pp. 559-565 ◽  
Author(s):  
J. Mast ◽  
C. Nanbru ◽  
T. van den Berg ◽  
G. Meulemans
Keyword(s):  
Electron Microscopy ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Goblet Cells ◽  
Ultrastructural Level ◽  
Ultrastructural Changes ◽  
Ciliated Cells ◽  
Specific Pathogen ◽  
And Function

The progression of tracheal lesions induced by vaccination of day-old specific pathogen-free chicks with the La Sota strain of Newcastle disease virus (NDV) was examined by relating surface changes as observed by scanning electron microscopy with subcellular changes seen by transmission electron microscopy. NDV infection resulted in hypertrophy of goblet cells, their rupture, and the formation of excess mucus. Activation of goblet cells peaked within 4 days postvaccination. Afterward, the activation levels gradually decreased. At the level of the ciliated cells, a marked increase in the proportion of nonciliated to ciliated cells and later an almost complete deciliation of the tracheal surface were observed because a simple squamous to cuboidal epithelium replaced the original pseudostratified epithelium. Fifteen days postvaccination, all epithelial damage was restored. Because the observed vaccination-induced lesions are detrimental to epithelial integrity and function as a barrier against invading microorganisms, they might explain at the ultrastructural level the secondary complications of vaccination with the La Sota strain against NDV

scholarly journals Molecular characterisation of Newcastle disease virus exotic isolate in East Java, Indonesia

2021 ◽  
Vol 24 (2) ◽  
pp. 191-199
Author(s):  
N. P. Kusumarahayu ◽  
N. Putri ◽  
R. Ernawati ◽  
J. Rahmahani ◽  
S. Suwarno ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Clinical Signs ◽  
Rt Pcr ◽  
Genetic Characteristics ◽  
Basic Work ◽  
Specific Pathogen ◽  
Baseline Information ◽  
Native Chickens

Newcastle disease virus (NDV) is ssRNA paramyxovirus causing clinical signs, varying from subclinical infections to 100% mortality in infected chickens. Haemagglutinin-neuraminidase (HN) protein has an important role related to infection and pathogenesis, therefore, the protein was characterised in this study. Samples were collected from 45 cloacal swabs of native chickens. They were isolated by inoculating in specific pathogen-free embryonated eggs. Molecular detection of NDV was done by reverse transcriptase polymerase chain reaction (RT-PCR) encoding HN protein. RT-PCR for HN gene of NDV generated DNA fragments sized 503 bp, which were then sequenced using ABI Prism. The results have shown that virus isolates were mostly lentogenic and might contribute to outbreak in East Java, Indonesia. Based on this fact, NDV infected native chickens can act as reservoir and contribute to outbreak in the poultry. Our study provides baseline information on genetic characteristics of NDV circulating in East Java and serves as a basic work for further research.

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