scholarly journals Clostridium difficile PCR ribotypes 001 and 176 – the common denominator of C. difficile infection epidemiology in the Czech Republic, 2014

2016 ◽  
Vol 21 (29) ◽  
Author(s):  
Marcela Krutova ◽  
Jana Matejkova ◽  
Ed J Kuijper ◽  
Pavel Drevinek ◽  
Otakar Nyc ◽  
...  
Keyword(s):  
Czech Republic ◽  
Clostridium Difficile ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Common Denominator ◽  
The Czech Republic ◽  
Testing Frequency ◽  
Bed Days ◽  
The Mean ◽  
Testing Protocols

In 2014, 18 hospitals in the Czech Republic participated in a survey of the incidence of Clostridium difficile infections (CDI) in the country. The mean CDI incidence was 6.1 (standard deviation (SD):7.2) cases per 10,000 patient bed-days and 37.8 cases (SD: 41.4) per 10,000 admissions. The mean CDI testing frequency was 39.5 tests (SD: 25.4) per 10,000 patient bed-days and 255.8 tests (SD: 164.0) per 10,000 admissions. A total of 774 C. difficile isolates were investigated, of which 225 (29%) belonged to PCR ribotype 176, and 184 isolates (24%) belonged to PCR ribotype 001. Multilocus variable-number tandem repeat analysis (MLVA) revealed 27 clonal complexes formed by 84% (190/225) of PCR ribotype 176 isolates, and 14 clonal complexes formed by 77% (141/184) of PCR ribotype 001 isolates. Clonal clusters of PCR ribotypes 176 and 001 were observed in 11 and 7 hospitals, respectively. Our data demonstrate the spread of two C. difficile PCR ribotypes within 18 hospitals in the Czech Republic, stressing the importance of standardising CDI testing protocols and implementing mandatory CDI surveillance in the country.

Clostridium difficile in piglets in the Czech Republic

2012 ◽  
Vol 57 (2) ◽  
pp. 159-161 ◽  
Author(s):  
Jana Goldová ◽  
Anna Malinová ◽  
Alexander Indra ◽  
Libor Vítek ◽  
Pavel Branny ◽  
...  
Keyword(s):  
Czech Republic ◽  
Clostridium Difficile ◽  
The Czech Republic

scholarly journals The Colonisation of Calves in Czech Large-Scale Dairy Farms by Clonally-Related Clostridioides difficile of the Sequence Type 11 Represented by Ribotypes 033 and 126

2020 ◽  
Vol 8 (6) ◽  
pp. 901
Author(s):  
Martina Masarikova ◽  
Ivana Simkova ◽  
Martin Plesko ◽  
Veronika Eretova ◽  
Marcela Krutova ◽  
...  
Keyword(s):  
Large Scale ◽  
Variable Number Tandem Repeat ◽  
Dairy Farms ◽  
Variable Number ◽  
Sequence Type ◽  
The Czech Republic ◽  
Holstein Breed ◽  
Clostridioides Difficile ◽  
Faecal Samples ◽  
The One

To investigate a possible Clostridioides difficile reservoir in the Czech Republic, we performed a study in 297 calves from 29 large-scale dairy farms. After enrichment, faecal samples were inoculated onto selective agar for C. difficile. From the 297 samples, 44 C. difficile isolates were cultured (prevalence of 14.8%, 10 farms). The Holstein breed and use of digestate were associated with C. difficile colonisation (p ˂ 0.05). C. difficile isolates belonged to the ribotype/sequence type: RT033/ST11 (n = 37), RT126/ST11 (n = 6) and RT046/ST35 (n = 1). A multiple-locus variable-number tandem-repeat analysis revealed four clonal complexes of RT033 isolates and one clonal complex of RT126 isolates. All isolates were sensitive to amoxicillin, metronidazole and vancomycin. Forty isolates were resistant to ciprofloxacin, twenty-one to clindamycin, seven to erythromycin, seven to tetracycline and six to moxifloxacin. Moxifloxacin resistant isolates revealed an amino-acid substitution Thr82Ile in the GyrA. In conclusion, the calves of Holstein breed from farms using digestate as a product of bio-gas plants are more likely to be colonised by clonally-related C. difficile of ST 11 represented by ribotypes 033 and 126. The identified resistance to moxifloxacin with a Thr82Ile substitution in the GyrA highlights the need for further monitoring by the “One health approach”.

scholarly journals Still too little, too late? Ten years of growth hormone therapy baseline data from the NordiNet® International Outcome Study

2018 ◽  
Vol 31 (5) ◽  
pp. 521-532 ◽  
Author(s):  
Michel Polak ◽  
Daniel Konrad ◽  
Birgitte Tønnes Pedersen ◽  
Gediminas Puras ◽  
Marta Šnajderová
Keyword(s):  
Growth Hormone ◽  
Czech Republic ◽  
Standard Deviation Score ◽  
Outcome Study ◽  
Gh Treatment ◽  
The Czech Republic ◽  
Serbia And Montenegro ◽  
The Mean ◽  
Late Start ◽  
The Uk

AbstractBackground:We investigated time trends in age, gender, growth hormone (GH) dose and height standard deviation score (SDS) in children with GH deficiency (GHD), born small for gestational age (SGA) or with Turner syndrome (TS) starting GH treatment.Methods:Data were obtained from children enrolled in the NordiNet®International Outcome Study (IOS) between 2006 and 2015 in the Czech Republic, France, Germany, Serbia and Montenegro (all indications), and Switzerland and the UK (GHD only). Trends were analyzed by linear regression. Patients were divided by age into early-, medium- or late-start groups in three different time periods.Results:Approximately one-third of children starting treatment for GHD were girls, with no apparent increase in proportion over time. The mean baseline age for starting treatment decreased significantly (p<0.001) for both GHD and SGA in the Czech Republic and Germany. In the other countries studied, over 40% of children started treatment for GHD and SGA late (girls >10, boys >11 years) between 2013 and 2015. The mean baseline GH doses were largely within recommended ranges for GHD and SGA, but below the lowest recommended starting dose for TS in almost every year since 2011 except in France.Conclusions:Approximately one-third of children starting treatment for GHD were girls. Between 2013 and 2015, more than 40% of children started treatment for GHD and SGA late except in Germany and the Czech Republic. TS patients received below-recommended doses. These results highlight the need for earlier identification of short stature in children, particularly girls, and for dose optimization in TS.

scholarly journals Comparison of the growth pattern of black walnut (Juglans nigra L.) in two riparian forests in the region of South Moravia, Czech Republic

2011 ◽  
Vol 57 (No. 3) ◽  
pp. 107-113 ◽  
Author(s):  
L. Šálek ◽  
P. Hejcmanová
Keyword(s):  
Czech Republic ◽  
Growth Pattern ◽  
Tree Height ◽  
Management Plan ◽  
Black Walnut ◽  
Riparian Forests ◽  
Juglans Nigra ◽  
The Czech Republic ◽  
The Mean ◽  
South Moravia

The black walnut (Juglans nigra L.), an introduced species into the Czech Republic, is planted for its production of valuable timber. A systematic study of the black walnut growth rate at different localities and consequently the creation of standard volume tables under Central European conditions therefore appear to be of high relevance. The aim of our study was to reveal the black walnut growth pattern in its pure stands in two riparian forests along the Morava and Svratka River (Str&aacute;žnice locality and Židlochovice locality, respectively). To describe and to compare relationships among diameter at breast height (dbh), tree height and age, we used a forest management plan and measured 573 and 670 trees in pure sample stands at Str&aacute;žnice and Židlochovice, respectively. We found out that the measured mean DBH and mean height were consistently higher in Str&aacute;žnice, however the relationships of DBH to height, age to DBH, and age to height showed the same pattern at both localities. The mean heights of trees were 16.3, 24.1, 28.7, 31.9, and 34.5 m in 20, 40, 60, 80, and 100 year age classes and were consistently taller in comparison with other, mainly European, regions. Therefore our results suggest the high potential of black walnut in timber production in riparian forests in the Czech Republic, mainly in the South Moravian region.

scholarly journals Resistance behaviour of Septoria tritici to some fungicides in the territory of the Czech Republic

2012 ◽  
Vol 58 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Ludvik Tvaružek ◽  
P. Horáková ◽  
L. Ji
Keyword(s):  
Czech Republic ◽  
Septoria Tritici ◽  
Resistance Development ◽  
The Czech Republic ◽  
Colony Diameter ◽  
Dmi Fungicides ◽  
The Mean ◽  
Different Parts ◽  
Pathogen Strains

Reduction of colony diameter using two DMI-fungicides and one strobilurine fungicide was evaluated in the <i>Septoria tritici</i> population. In an <i>in vitro</i> assay, 57 strains of <i>S. tritici</i> originating from different parts of the Czech Republic were grown on agar plates containing different concentrations of fungicides. The mean EC<sub>50</sub> values of strains were analysed for flusilazole (Capitain), metconazole (Caramba) and one strobilurine fungicide: azoxystrobin (Amistar). ED 50 values for metconazole were low. There were highly significant correlation in reaction of pathogen strains to metconazole and flusilazole. Some isolates showed significant higher level of resistance to azoxystrobin. The data will be used in a continuing survey of resistance development in the <i>S. tritici</i> population originating from the territory of the Czech Republic.

scholarly journals Gross value yield potential of coppice, high forest and model conversion of high forest to coppice on best sites

2011 ◽  
Vol 57 (No. 12) ◽  
pp. 536-546 ◽  
Author(s):  
M. Kneifl ◽  
J. Kadavý ◽  
R. Knott
Keyword(s):  
Czech Republic ◽  
The Other ◽  
Yield Potential ◽  
Fuel Wood ◽  
Annual Increment ◽  
Site Class ◽  
The Czech Republic ◽  
The Mean ◽  
Model Conversion ◽  
Mean Annual Increment

&nbsp;Based on yield tables for oak high forest and oak coppice (both first site class) and using assortment tables and assortment prices in the Czech Republic in 2009, a set of variants of conversion of high forest to coppice was simulated. Average annual cut and average gross value of annual cut of such conversions were compared with those of well-established (in terms of the age structure balance) variants of coppice and high forest. Under the existing ratio of assortment prices, established coppice does not reach the gross value yield of high forest. No variant of simulated conversions was more financially profitable than the initial high forest. Furthermore, we found out that a +16.8% increase of the current fuel wood price would counterbalance the mean annual increment of gross value of the best coppice and the worst oak high forest variant. On the other hand, a +164.7% fuel wood price increase would be necessary to counterbalance the mean annual increment of gross value of the worst coppice and the best high forest variants. &nbsp;

scholarly journals Multilocus Variable-Number Tandem-Repeat Analysis for Investigation of Clostridium difficile Transmission in Hospitals

2006 ◽  
Vol 44 (7) ◽  
pp. 2558-2566 ◽  
Author(s):  
J. W. Marsh ◽  
M. M. O'Leary ◽  
K. A. Shutt ◽  
A. W. Pasculle ◽  
S. Johnson ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Repeat Analysis
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