scholarly journals Escherichia coli O157 infections and unpasteurised milk

2001 ◽  
Vol 6 (10) ◽  
pp. 147-151 ◽  
Author(s):  
F Allerberger ◽  
M Wagner ◽  
P Schweiger ◽  
H- P. Rammer ◽  
A Resch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dairy Cow ◽  
Shiga Toxin ◽  
Haemolytic Uraemic Syndrome ◽  
Escherichia Coli O157 ◽  
Molecular Subtyping ◽  
Animal Isolates

We report on two children with Escherichia coli O157 infection, one of whom developed haemolytic uraemic syndrome (HUS). Both had drunk raw cows’ or goats’ milk in the week before their illness. Molecular subtyping identified a sorbitol fermenting Escherichia coli O157:H isolate from a dairy cow. This isolate differed from Shiga toxin producing O157:H strains isolated from the 6 year old boy with HUS. This result underlines the need to search for other causes of infection, despite documented consumption of unpasteurised milk. In the second patient, human sorbitol non-fermenting O157:H isolates and animal isolates from goats were indistinguishable. The isolation of indistinguishable sorbitol non-fermenting Escherichia coli O157:H from contact animals supports the association between HUS and consumption of raw goats’ milk, and re-emphasises the importance of pasteurising milk.

scholarly journals Comparison between human and animal isolates of Shiga toxin-producing Escherichia coli O157 from Australia

2002 ◽  
Vol 128 (3) ◽  
pp. 357-362 ◽  
Author(s):  
N. FEGAN ◽  
P. DESMARCHELIER
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Human Infection ◽  
Pulsed Field Gel Electrophoresis ◽  
Toxin Gene ◽  
Escherichia Coli O157 ◽  
Animal Population ◽  
E Coli ◽  
Virulence Markers ◽  
Animal Isolates

There is very little human disease associated with enterohaemorrhagic Escherichia coli O157 in Australia even though these organisms are present in the animal population. A group of Australian isolates of E. coli O157:H7 and O157:H- from human and animal sources were tested for the presence of virulence markers and compared by XbaI DNA macrorestriction analysis using pulsed-field gel electrophoresis (PFGE). Each of 102 isolates tested contained the gene eae which encodes the E. coli attaching and effacing factor and all but one carried the enterohaemolysin gene, ehxA, found on the EHEC plasmid. The most common Shiga toxin gene carried was stx2c, either alone (16%) or in combination with stx1 (74%) or stx2 (3%). PFGE grouped the isolates based on H serotype and some clusters were source specific. Australian E. coli O157:H7 and H- isolates from human, animal and meat sources carry all the virulence markers associated with EHEC disease in humans therefore other factors must be responsible for the low rates of human infection in Australia.

scholarly journals Sorbitol-fermenting Shiga toxin-producing Escherichia coli O157: indications for an animal reservoir

2005 ◽  
Vol 134 (4) ◽  
pp. 719-723 ◽  
Author(s):  
D. ORTH ◽  
K. GRIF ◽  
M. P. DIERICH ◽  
R. WÜRZNER
Keyword(s):  
Escherichia Coli ◽  
Clinical Diagnosis ◽  
Shiga Toxin ◽  
Haemolytic Uraemic Syndrome ◽  
Stool Specimen ◽  
Farm Animals ◽  
Molecular Characteristics ◽  
Escherichia Coli O157 ◽  
Animal Reservoir ◽  
Faecal Samples

This study investigates a sorbitol-fermenting enterohaemorrhagic Escherichia coli (SF EHEC) O157 infection in a farmer's family in the Austrian province of Salzburg. The investigation commenced after a 10-month-old boy was admitted to hospital with the clinical diagnosis of a haemolytic–uraemic syndrome (HUS) and his stool specimen grew SF EHEC O157:H−. In a subsequent environmental survey, a stool specimen of the 2-year-old brother and faecal samples of two cattle from the family's farm were also found to be positive for SF EHEC O157:H−. All four isolates had indistinguishable phenotypic and molecular characteristics and were identical to the first strain detected in Bavaria in 1988. Despite identical isolates being demonstrated in Bavaria after 1988, and until this report, increased surveillance in neighbouring Austria had not found this organism. We propose that the strain may have recently spread from Bavaria to Austria. Although SF EHEC O157:H− strains are still rare, they may represent a considerable health threat as they can spread from farm animals to humans and between humans.

scholarly journals Escherichia coli O157 infection associated with a petting zoo

2002 ◽  
Vol 129 (2) ◽  
pp. 295-302 ◽  
Author(s):  
A. E. HEUVELINK ◽  
C. VAN HEERWAARDEN ◽  
J. T. M. ZWARTKRUIS-NAHUIS ◽  
R. VAN OOSTEROM ◽  
K. EDINK ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Haemolytic Uraemic Syndrome ◽  
Escherichia Coli O157 ◽  
Zoonotic Infections ◽  
Faecal Samples ◽  
Animal Contact ◽  
Shiga Toxin 2 ◽  
On Farm ◽  
Animal Isolates

A young child was admitted to hospital with haemolytic-uraemic syndrome caused by infection with a Shiga toxin 2-producing strain of Escherichia coli (STEC) O157. Five days before he became ill, the child had visited a small petting zoo. STEC O157 strains were isolated from faecal samples from goats and sheep housed on the farm. The human and the animal isolates were indistinguishable by molecular subtyping. The petting zoo voluntarily closed temporarily to prevent further cases of infection. Two out of 11 other, randomly selected petting zoos (including one deer park) visited subsequently, tested positive. Furthermore, during the study period there was one more notification of STEC O157 infection possibly linked with a farm visit. Although STEC O157 was indeed found in the petting zoo associated with this patient, transmission through animal contact could not be confirmed because the human isolate was not available for subtyping. The case study and the results of the other on-farm investigations highlight the risk of acquiring severe zoonotic infections during visits to petting zoos.

scholarly journals Shiga Toxin-Producing Escherichia coli O157 Shedding Dynamics in an Australian Beef Herd

2017 ◽  
Vol 4 ◽  
Author(s):  
Christina Ahlstrom ◽  
Petra Muellner ◽  
Geraldine Lammers ◽  
Meghan Jones ◽  
Sophie Octavia ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Escherichia Coli O157 ◽  
Beef Herd
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