Background:
The leaves of Annona reticulata Linn. have been traditionally used by the tribes of
Assam as a source of medicine to mitigate a range of health ailments including diabetes and
obesity.
Objectives:
The current study aimed to evaluate the anti-diabetic and anti-hyperlipidemic
potential of bioactive fractions isolated from the methanolic extract of Annona reticulata Linn.
leaves using Nicotinamide + Streptozotocin (60 mg/kg, i.p.) induced diabetic rats.
Methods:
The partially purified bioactive fractions, namely F1, F2, F3 and F4 were administered to
diabetic rats with the dose of 200 mg/kg, per oral (p.o.) and the effect of the fractions on serum
glucose were studied up to 21 days. The potent fractions were further subjected for spectral
analysis for identification of the isolated active compounds.
Results:
The in-vivo anti-diabetic activity of the isolated fractions F2 and F3 were found
significant controlling blood glucose level, alike glibenclamide. Interestingly, F2 and F3 treated
animals were found significant in restoring the lipid and liver enzymes profile in streptozotocin
challenge rats. Further, spectral analysis revealed that F2 and F3 were comprises Quercetin and
Gallic acid, respectively.
Conclusion:
Outcome of finding demonstrate the anti-diabetic and anti-hyperlipidemic potential
of the isolates/fractions of A. reticulata, which were found enriched in polyphenolics including
Quercetin and Gallic acid; and provides logistic behind the traditional use of the A. reticulata
against Diabetes and obesity.
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