scholarly journals Molecular-genetic identification of arbuscular mycorrhiza fungi from Teberda natural reserve

2020 ◽  
Author(s):  
А. А. Kryukov ◽  
A. O. Gorbunova ◽  
Sh. K. Kurbanniyazov ◽  
Yu. V. Mikhaylova ◽  
A. V. Rodionov ◽  
...  
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Molecular Genetic ◽  
Its Region ◽  
Illumina Miseq ◽  
Genetic Identification ◽  
Universal Primers ◽  
North Caucasus ◽  
Arbuscular Mycorrhiza Fungi ◽  
Natural Reserve ◽  
Molecular Genetic Identification

Arbuscular mycorrhiza fungi of soil samples from North Caucasus were identified via Illumina Miseq and universal primers for ITS region. It was shown, that both ITS1 and ITS2 are necessary for identification.

scholarly journals Illumina-based identification of arbuscular mycorrhizal fungi from Karachay-Cherkessia soils for development of bio-fertilizers

2021 ◽  
Vol 285 ◽  
pp. 03001
Author(s):  
Andrey Yurkov ◽  
Alexey Kryukov ◽  
Yulia Mikhaylova ◽  
Peter Zhurbenko
Keyword(s):  
Mycorrhizal Fungi ◽  
Biological Diversity ◽  
Arbuscular Mycorrhizal ◽  
Its Region ◽  
Am Fungi ◽  
Illumina Miseq ◽  
Universal Primers ◽  
Its2 Region ◽  
North Caucasus ◽  
The Future

The aim of the study was to investigate the species diversity of AM fungi in different parts of the North Caucasus, biodiversity hotspot, the center of the world’s biological diversity. Samples were taken from 5 locations (stationary trial plots, STPs) in different ecosystems and at various altitudes. Identification was performed using sequencing for ITS1 and ITS2 regions, amplified with universal primers, Illumina MiSeq was employed. 19 genera of AM fungi were found on all STPs. The work did not reveal a correlation between the altitude and the species composition of AM fungi. At the same time, it should be assumed that a correlation could be found between the biodiversity of AM fungi and the type of ecosystem, which should be done in the future. The study shows it is necessary to use an analysis for both ITS regions, since the data obtained for each ITS region differ and complement each other. Analysis for the ITS2 region revealed 1.3 times more virtual taxa than for the ITS1, while the number of OTUs identified per species was similar for both regions. The highest biodiversity of AM fungi was found in STP #3 (with meadow flora). Only 4 species (Rhizophagus irregularis, R. intraradices, Paraglomus laccatum, and Claroideoglomus claroideum) were found on all five analyzed STPs. We found unexpectedly that with such a high biodiversity among the identified fungi, no different species were found in the Paraglomus genus, all the sequences of Paraglomus belonged to Paraglomus laccatum, whereas at least 9 species are distinguished in the genus by morphology. Further research will allow us to identify new strains of AM fungi, the efficiency of which may be higher than already studied ones. In the future this will make it possible to create more effective microbial biofertilizers for agriculture.

Molecular genetic identification of isolates of the hepatitis A virus (HAV) from monkeys at Adler Primate Center

2018 ◽  
Vol 47 (2) ◽  
pp. 87-92 ◽  
Author(s):  
Dmitriy I. Dogadov ◽  
Lydia I. Korzaya ◽  
Anastasia A. Karlsen ◽  
Karen K. Kyuregyan
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Molecular Genetic ◽  
Genetic Identification ◽  
Molecular Genetic Identification

scholarly journals Selecting of Polymorphic Loci of Genome for Identification of Populations of Pinus sylvestris L. on East-Europe Plain

2019 ◽  
Vol 5 (5) ◽  
pp. 25-30
Author(s):  
Ya. Prishnivskaya ◽  
E. Nassonova ◽  
Yu. Vasileva ◽  
S. Boronnikova
Keyword(s):  
Pinus Sylvestris ◽  
Molecular Genetic ◽  
Genetic Identification ◽  
East European Plain ◽  
Data Bases ◽  
East Europe ◽  
Polymorphic Loci ◽  
East European ◽  
Molecular Genetic Identification

10 pairs of primers from 8 related Pinus sylvestris L. populations collected on East-European plain to 10 genes and 4 primer’s pairs to 4 loci of uncoding clDNA regions. 2 loci of uncoding clDNA regions (psbA-trnH, trnL-trnF) were selected from tested 14 primer’s pairs. These two loci are most polymorphic and has homologous consistencies in data bases. Therefore, these loci is recommended for molecular–genetic identification of related Pinus sylvestris L. populations on East–European plain.

scholarly journals Study of European and Czech populations of potato cyst nematodes (Globodera rostochiensis and G. pallida) by RAPD method

2011 ◽  
Vol 50 (No. 2) ◽  
pp. 70-74 ◽  
Author(s):  
P. Sedlák ◽  
M. Melounová ◽  
S. Skupinová ◽  
P. Vejl ◽  
J. Domkářová
Keyword(s):  
Animal Species ◽  
Molecular Genetic ◽  
Globodera Rostochiensis ◽  
Globodera Pallida ◽  
Genetic Identification ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Genetic Dissimilarity ◽  
Solanaceous Plants ◽  
Molecular Genetic Identification

Potato cyst nematodes (PCN) are the big problem in worldwide planting of potatoes and another Solanaceous plants. Identification of individual pathotypes according to international scheme is very demanding but a very important part of the phytosanitary process to control these pests. Molecular genetic identification of different plant and animal species or individuals is a very interesting way at the present time and let’s hope that it will be important in future. This report presents results of the RAPD study of nine different real PCN populations. There were five Globodera rostochiensis populations and four G. pallida populations. Pathotypes Ro2, Ro2/3, Ro4, Ro5, Pa2 and Pa3 were from European populations; population Ro1 and X were of Czech provenance. Genetics variable of these populations was described by a set of six decameric primers (OPA 07, OPG 03, OPG 05, OPG 08, OPG 10 and OPG 13). Genetic dissimilarity was by Gel Manager for Windows evaluated. Detectable differences behind all populations were found and the dendrogram was compiled. The unknown population X was sorted into group of Globodera pallida species subgroup of Pa2 consequently.

Molecular genetic identification of southern hemisphere beaked whales (Cetacea: Ziphiidae)

1998 ◽  
Vol 7 (6) ◽  
pp. 687-694 ◽  
Author(s):  
M. L. DALEBOUT ◽  
A. VAN HELDEN ◽  
K. VAN WAEREBEEK ◽  
C. S. BAKER
Keyword(s):  
Southern Hemisphere ◽  
Molecular Genetic ◽  
Genetic Identification ◽  
Beaked Whales ◽  
Molecular Genetic Identification

The first molecular genetic identification of the tularemia pathogen in Ixodes trianguliceps Bir. ticks in Russia

2016 ◽  
Vol 31 (2) ◽  
pp. 82-86
Author(s):  
M. I. Kormilitsyna ◽  
E. I. Korenberg ◽  
Yu. V. Kovalevskii ◽  
I. S. Meshcheryakova
Keyword(s):  
Molecular Genetic ◽  
Genetic Identification ◽  
Ixodes Trianguliceps ◽  
Molecular Genetic Identification

scholarly journals Molecular genetic identification of sensu stricto strains from African sorghum beer

2003 ◽  
Vol 3 (2) ◽  
pp. 177-184 ◽  
Author(s):  
E NAUMOVA ◽  
I KORSHUNOVA ◽  
L JESPERSEN ◽  
G NAUMOV
Keyword(s):  
Molecular Genetic ◽  
Sensu Stricto ◽  
Genetic Identification ◽  
Molecular Genetic Identification

Autosomal recessive early-onset parkinsonism with diurnal fluctuation: clinicopathologic characteristics and molecular genetic identification

2000 ◽  
Vol 22 ◽  
pp. 87-91 ◽  
Author(s):  
Yasuhiro Yamamura ◽  
Nobutaka Hattori ◽  
Hiroto Matsumine ◽  
Shigeki Kuzuhara ◽  
Yoshikuni Mizuno
Keyword(s):  
Early Onset ◽  
Autosomal Recessive ◽  
Molecular Genetic ◽  
Genetic Identification ◽  
Diurnal Fluctuation ◽  
Clinicopathologic Characteristics ◽  
Molecular Genetic Identification

Hair snaring and molecular genetic identification for reconstructing the spatial structure of Eurasian lynx populations

2013 ◽  
Vol 78 (2) ◽  
pp. 118-126 ◽  
Author(s):  
Francesca Davoli ◽  
Krzysztof Schmidt ◽  
Rafał Kowalczyk ◽  
Ettore Randi
Keyword(s):  
Spatial Structure ◽  
Molecular Genetic ◽  
Genetic Identification ◽  
Eurasian Lynx ◽  
Molecular Genetic Identification
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