scholarly journals PURIFICATION OF RECOMBINANT PRES2-S PROTEIN, THE SURFACE ANTIGEN OF HUMAN HEPATITIS B VIRUS (HBV) EXPRESSED IN BOMBYX MORI LARVAE

2019 ◽  
pp. 7-10
Author(s):  
J. Abdurakhmanov ◽  
S. Sasmakov ◽  
O. Ashirov ◽  
S. Khasanov ◽  
F. Eshboev ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Bombyx Mori ◽  
Surface Antigen ◽  
S Protein ◽  
Human Hepatitis ◽  
B Virus

scholarly journals Expression Level of Small Envelope Protein in Addition to Sequence Divergence inside Its Major Hydrophilic Region Contributes to More Efficient Surface Antigen Secretion by Hepatitis B Virus Subgenotype D2 than Subgenotype A2

Viruses ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 967
Author(s):  
Qianru Wang ◽  
Shuwen Fu ◽  
Jing Zhang ◽  
Quan Yuan ◽  
Jisu Li ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Envelope Protein ◽  
Hepatitis B Surface Antigen ◽  
Site Directed Mutagenesis ◽  
Enzyme Linked Immunosorbent Assay ◽  
S Protein ◽  
Huh7 Cells ◽  
B Virus

Hepatitis B surface antigen (HBsAg) promotes persistent hepatitis B virus (HBV) infection. It primarily corresponds to small (S) envelope protein secreted as subviral particles. We previously found that genotype D clones expressed less S protein than genotype A clones but showed higher extracellular/intracellular ratio of HBsAg suggesting more efficient secretion. The current study aimed to characterize the underlying mechanism(s) by comparing a subgenotype A2 clone (geno5.4) with a subgenotype D2 clone (geno1.2). Five types of full-length or subgenomic constructs were transfected to Huh7 cells at different dosage. HBsAg was quantified by enzyme linked immunosorbent assay while envelope proteins were detected by Western blot. We found that ratio of extracellular/intracellular HBsAg decreased at increasing amounts of DNA transfected. Conflicting findings from two types of subgenomic construct confirmed stronger secretion inhibitory effect of the genotype D-derived large envelope protein. Chimeric constructs followed by site-directed mutagenesis revealed geno1.2 specific V118/T127 and F161/A168 in the S protein as promoting and inhibitory of HBsAg secretion, respectively. In conclusion, more efficient HBsAg secretion by subgenotype D2 than subgenotype A2 is attributed to lower level of S protein expression in addition to V118 and T127 in S protein, although its F161 and A168 sequences rather reduce HBsAg secretion.

scholarly journals Use of Pre-S Protein-Containing Hepatitis B Virus Surface Antigens and a Powerful Adjuvant To Develop an Immune Therapy for Chronic Hepatitis B Virus Infection

2011 ◽  
Vol 19 (2) ◽  
pp. 120-127 ◽  
Author(s):  
Jung Sun Yum ◽  
Byung Cheol Ahn ◽  
Hyun Jin Jo ◽  
Dong Yeon Kim ◽  
Ki Hyun Kim ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Immune Therapy ◽  
Surface Antigens ◽  
Virus Surface ◽  
S Protein ◽  
Chronic Hepatitis B Virus ◽  
B Virus

ABSTRACTA hepatitis B virus (HBV) vaccine has been developed using a new adjuvant and HBV surface antigens produced from a CHO cell line. The purified HBV surface antigens are composed of L protein, M protein, and S protein in a mixture of 20- and 40-nm-diameter particles and filamentous forms. This HBV surface antigen, formulated with L-pampo, a proprietary adjuvant, induced 10 times more antibody than the same antigen with alum and was capable of inducing strong immune responses in three different HBV transgenic mice. In spite of the presence of a large amount of HBV antigen in the blood, no antibody against HBV surface antigen was normally detected in these transgenic mice. After immunization, the HBV antigen was also cleared from the blood.

Application of Functional Microsphere in Human Hepatitis B Virus Surface Antigen Detection

2014 ◽  
Vol 14 (5) ◽  
pp. 3348-3355 ◽  
Author(s):  
Ningning Ma ◽  
Chao Ma ◽  
Nianyue Wang ◽  
Chuanyan Li ◽  
Sauli Elingarami ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Antigen Detection ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
Human Hepatitis ◽  
B Virus

scholarly journals Phospholipid interactions of the putative fusion peptide of hepatitis B virus surface antigen S protein

1995 ◽  
Vol 76 (2) ◽  
pp. 301-308 ◽  
Author(s):  
I. Rodriguez-Crespo ◽  
E. Nunez ◽  
J. Gomez-Gutierrez ◽  
B. Yelamos ◽  
J. P. Albar ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Fusion Peptide ◽  
Virus Surface ◽  
S Protein ◽  
Virus Surface Antigen ◽  
B Virus
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