Comparison of Co-Presence of Epstein-Barr Virus and High-Risk Human Papillomaviruses in Colorectal Cancers in the Middle East Region

2020 ◽  
Author(s):  
Karim Nagi ◽  
Ishita Gupta ◽  
Hamda A Al-Thawadi ◽  
Ayesha Jabeen ◽  
Mohammed I. Malk ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
High Risk ◽  
Epstein Barr Virus ◽  
Human Papillomaviruses ◽  
Colorectal Cancers ◽  
Barr Virus ◽  
Human Carcinomas ◽  
Epstein Barr ◽  
Invasive Carcinomas ◽  
High Risk Hpv

Background: Several studies have shown the presence of onco viral DNA in colorectal tumor tissues. Viral infection by onco-viruses such as Human papillomaviruses (HPVs) and Epstein–Barr virus (EBV) are well-known to be involved in the onset and/or progression of numerous human carcinomas. Methods: We explored the co-presence of high-risk HPVs and EBV in a cohort of colorectal cancer samples from Lebanon (94) and Syria (102) by PCR, immunohistochemistry and tissue microarray. Results: The results of the study point out that 54% of colorectal cancer cases in Syria are positive for high-risk HPVs, while 30% of the cases in Lebanon are positive for these viruses; the most frequent high-risk HPV types in these populations are 16, 18, 31, 33 and 35. Analysis of LMP1 showed similar results in both populations; 36% of Syrian and 31% of Lebanese samples. Additionally, we report that EBV and high-risk HPVs are co-present in these samples. In Syrian samples, EBV and HPVs are co-present in 16% of the population, however, in the Lebanese samples, 20% of the cases are positive for both EBV and HPVs; their co-presence is associated with high/intermediate grade invasive carcinomas. Conclusion: These data suggest that EBV and high-risk HPVs are co-present in human colorectal cancers where they can cooperate in the progression of these cancers. Nevertheless, further studies are needed to elucidate the role of those oncoviruses in the development of human colorectal carcinomas.

scholarly journals High-Risk Human Papillomaviruses and Epstein–Barr Virus in Colorectal Cancer and Their Association with Clinicopathological Status

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 452 ◽  
Author(s):  
Ishita Gupta ◽  
Halema Al Farsi ◽  
Ayesha Jabeen ◽  
Faruk Skenderi ◽  
Hamda Al-Thawadi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
High Risk ◽  
Epstein Barr Virus ◽  
Human Papillomaviruses ◽  
Sample Population ◽  
Barr Virus ◽  
The Status ◽  
Epstein Barr ◽  
High Risk Hpv

Colorectal cancer (CRC) is a common malignancy with a high mortality rate worldwide. It is a complex, multifactorial disease that is strongly impacted by both hereditary and environmental factors. The role of microbes (e.g., viruses) in the pathogenesis of CRC is poorly understood. In the current study, we explored the status of high-risk human papillomaviruses (HPV) and Epstein–Barr virus (EBV) in a well-defined CRC cohort using immunohistochemistry and polymerase chain reaction assays. Our data showed that high-risk HPVs were common (~80%) and EBV had a low presence (14–25%) in the CRC samples. The most common high-risk HPVs are HPV16, 31, 18, 51, 52 and 45 genotypes. The co-presence of high-risk HPV and EBV was observed in ~16% of the sample population without any significant association with the clinicopathological variables. We conclude that high-risk HPVs are very prevalent in CRC samples while EBV positivity is relatively low. The co-expression of the two viruses was observed in a minority of cases and without any correlation with the studied parameters. Further studies are necessary to confirm the clinical relevance and potential therapeutic (preventive) effects of the observations reported herein.

scholarly journals Co-presence of Epstein–Barr virus and high-risk human papillomaviruses in Syrian colorectal cancer samples

2020 ◽  
Vol 16 (10) ◽  
pp. 2403-2407 ◽  
Author(s):  
Mohammed I. Malki ◽  
Ishita Gupta ◽  
Queenie Fernandes ◽  
Tahar Aboulkassim ◽  
Amber Yasmeen ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
High Risk ◽  
Epstein Barr Virus ◽  
Human Papillomaviruses ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Human Papillomaviruses and Epstein–Barr Virus Interactions in Colorectal Cancer: A Brief Review

Pathogens ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 300 ◽  
Author(s):  
Queenie Fernandes ◽  
Ishita Gupta ◽  
Semir Vranic ◽  
Ala-Eddin Al Moustafa
Keyword(s):  
Colorectal Cancer ◽  
High Risk ◽  
Cancer Progression ◽  
Epstein Barr Virus ◽  
Human Papillomaviruses ◽  
Colorectal Carcinogenesis ◽  
Mesenchymal Transition ◽  
Barr Virus ◽  
Epstein Barr

Human papillomaviruses (HPVs) and the Epstein–Barr virus (EBV) are the most common oncoviruses, contributing to approximately 10%–15% of all malignancies. Oncoproteins of high-risk HPVs (E5 and E6/E7), as well as EBV (LMP1, LMP2A and EBNA1), play a principal role in the onset and progression of several human carcinomas, including head and neck, cervical and colorectal. Oncoproteins of high-risk HPVs and EBV can cooperate to initiate and/or enhance epithelial-mesenchymal transition (EMT) events, which represents one of the hallmarks of cancer progression and metastasis. Although the role of these oncoviruses in several cancers is well established, their role in the pathogenesis of colorectal cancer is still nascent. This review presents an overview of the most recent advances related to the presence and role of high-risk HPVs and EBV in colorectal cancer, with an emphasis on their cooperation in colorectal carcinogenesis.

scholarly journals Copresence of High-Risk Human Papillomaviruses and Epstein–Barr Virus in Colorectal Cancer: A Tissue Microarray and Molecular Study from Lebanon

2021 ◽  
Vol 22 (15) ◽  
pp. 8118
Author(s):  
Karim Nagi ◽  
Ishita Gupta ◽  
Nawaf Jurdi ◽  
Amber Yasmeen ◽  
Semir Vranic ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
High Risk ◽  
Tissue Microarray ◽  
Epstein Barr Virus ◽  
Molecular Study ◽  
Human Papillomaviruses ◽  
Colorectal Carcinogenesis ◽  
Tissue Samples ◽  
Barr Virus ◽  
Epstein Barr

Colorectal cancer (CRC) is the third most common cause of cancer-related deaths worldwide. Human papillomaviruses (HPVs) and Epstein–Barr virus (EBV) have been reported to be present in different types of human cancers, including CRCs, where they can play a key role in the onset and/or progression of these cancers. Thus, we herein explored the prevalence of high-risk HPVs and EBV in a cohort of 94 CRC tissue samples and 13 colorectal normal tissues from the Lebanese population using polymerase chain reaction, immunohistochemistry, and tissue microarray methodologies. We found that high-risk HPVs are present in 64%, while EBV is present in 29% of our CRC samples. Additionally, our data showed that high-risk HPV types (16, 18, 35, 58, 51, 45, 52, 31, and 33) are the most frequent in CRC in the Lebanese cohort, respectively. Our data point out that HPVs and EBV are copresent in 28% of the samples. Thus, this study clearly suggests that high-risk HPVs and EBV are present/copresent in CRCs, where they could play an important role in colorectal carcinogenesis. Nevertheless, further investigations using a larger cohort are needed to elucidate the possible cooperation between these oncoviruses in the development of CRC.

scholarly journals Seroprevalence of Chlamydia trachomatis, herpes simplex 2, Epstein-Barr virus, hepatitis C and associated factors among a cohort of men ages 18–70 years from three countries

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0253005
Author(s):  
Shams Rahman ◽  
Deanna Wathington ◽  
Tim Waterboer ◽  
Michael Pawlita ◽  
Luisa L. Villa ◽  
...  
Keyword(s):  
Sexual Behavior ◽  
Hepatitis C ◽  
Herpes Simplex ◽  
High Risk ◽  
Epstein Barr Virus ◽  
Sexual Partners ◽  
Barr Virus ◽  
Hpv Types ◽  
Epstein Barr ◽  
High Risk Hpv

Objectives To estimate the seroprevalence of Chlamydia trachomatis (CT), herpes simplex type-2 (HSV2), hepatitis C (HCV), Epstein-Barr virus (EBV) and nine human papilloma virus (HPV) types, and investigated factors associated with the seropositivity among men from three countries (Brazil, Mexico and U.S). Methods Archived serum specimens collected at enrollment for n = 600 men were tested for antibodies against CT, HSV2, HCV, EBV, and 9-valent HPV vaccine types (6/11/16/18/31/33/45/52/58) using multiplex serologic assays. Socio-demographic, lifestyle and sexual behavior data at enrollment were collected through a questionnaire. Results Overall, 39.3% of the men were seropositive for CT, 25.4% for HSV2, 1.3% for HCV, 97.3% for EBV, 14.0% for at least one of the seven oncogenic HPV (types: 16/18/31/33/45/52/58), and 17.4% for HPV 6/11. In the unadjusted models, age, race, smoking, sexual behavior variables, and seropositivity for high-risk HPV were significantly associated with the seropositivity for CT. In multivariable analyses, self-reported black race, higher numbers of lifetime female/male sexual partners, current smoking, and seropositivity to high-risk HPV were significantly associated with increased odds of CT seropositivity. Odds of HSV2 seroprevalence were elevated among older men and those seropositive for high risk HPV. Conclusion Exposure to STIs is common among men. Prevention and screening programs should target high-risk groups to reduce the disease burden among men, and to interrupt the disease transmission to sexual partners.

scholarly journals High-risk human papillomaviruses and Epstein–Barr virus in breast cancer in Lebanese women and their association with tumor grade: a molecular and tissue microarray study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Karim Nagi ◽  
Ishita Gupta ◽  
Nawaf Jurdi ◽  
Ayesha Jabeen ◽  
Amber Yasmeen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
High Risk ◽  
Tissue Microarray ◽  
Epstein Barr Virus ◽  
Tumor Grade ◽  
Human Papillomaviruses ◽  
Breast Cancers ◽  
Human Breast ◽  
Barr Virus ◽  
Epstein Barr

Abstract Background High-risk human papillomaviruses (HPVs) are present and can cooperate with Epstein–Barr virus (EBV) to initiate and/or enhance the progression of several types of human carcinomas including cervical as well as head and neck; in parallel, it has been recently pointed out that these oncoviruses can be detected in human breast cancers. Thus, we herein explored the presence/co-presence of high-risk HPVs and EBV in breast cancer in Lebanese women. Methods A cohort of 102 breast cancer samples and 14 normal breast tissues were assessed for the presence of HPVs and EBV. Polymerase chain reaction (PCR) and immunohistochemistry (IHC) analysis in addition to tissue microarray (TMA) platform were used in this study. Results We found the presence of HPV in 66/102 (65%) of our samples, while EBV is present in 41/102 (40%) of the cohort. Additionally, our data showed that high-risk HPV types (52, 35, 58, 45, 16 and 51) are the most frequent in breast cancer in Lebanese women. Meanwhile, we report that high-risk HPVs and EBV are co-present in 30/102 (29%) of the samples; more significantly, our results indicate that their co-presence is associated with tumor grade (p = 0.03). Conclusion Our data revealed that HPVs and EBV are present/co-present in human breast cancer where they may play an important role in its development and/or progression; thus, we believe that further investigations are essential to confirm and elucidate the presence/co-presence of these oncoviruses and the underlying mechanisms of their interaction in breast carcinogenesis.

scholarly journals Co-Prevalence of Human Papillomavirus and Epstein Barr Virus in Healthy Blood Donors in Qatar

2020 ◽  
Author(s):  
Ishita Gupta ◽  
Ayesha Jabeen ◽  
Maria K. Smatti ◽  
Hamda A. Al-Thawadi ◽  
Gheyath K. Nasrallah ◽  
...  
Keyword(s):  
High Risk ◽  
Epstein Barr Virus ◽  
Blood Donors ◽  
Adult Population ◽  
Human Papillomaviruses ◽  
Healthy Population ◽  
Barr Virus ◽  
Hpv Types ◽  
Epstein Barr ◽  
Pcr Targeting

Introduction: Infections with human oncoviruses such as high-risk human papillomaviruses (HPVs) and Epstein-Barr virus (EBV) are globally prevalent in the adult population. Both viruses are strongly associated with several types of human carcinomas such as cervical, head and neck, nasopharyngeal and gastric. In the present study, we explored the prevalence of these two oncoviruses in the healthy population of Qatar. Methods: The study included 385 healthy blood donors that reflect diverse nationalities in the Qatari community (Qatar, Egypt, Syria, Jordan, Pakistan, and India). DNA was extracted from the peripheral blood and genotyping was done using PCR and nested-PCR targeting E6 and E7 as well as LMP1 genes of HPVs and EBV, respectively. Results: The age of participants (378 males and 7 females) ranged between 19 and 68 years (mean 37.12 ± 9.3 years). Our data indicate that 55% and 61% of the tested samples were HPVs and EBV positive, respectively. Moreover, we found that there was (40%) co-presence of both HPVs and EBV in our samples. The most common high-risk HPV types in Qatar included HPV 59 (55%), 31 (54%), 52 (49%), 51 (49%), 58 (47%) and 35 (46%). While, HPV 16 and 18 were detected in 38% and 36% of the samples, respectively. Notably, all samples showed multiple HPVs infections. Conclusion: Our study reveals for the first time a high prevalence of both EBV and HPVs among healthy individuals in Qatar. More significantly, most cases had multiple HPV types infection in addition to the co-presence of both viruses in a substantial proportion of the samples. Given the important possible cooperative role of these viruses in human carcinogenesis, preventive measures using available and upcoming vaccines are of paramount importance.

scholarly journals High-Risk Human Papillomavirus and Epstein–Barr Virus Coinfection: A Potential Role in Head and Neck Carcinogenesis

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1232
Author(s):  
Rancés Blanco ◽  
Diego Carrillo-Beltrán ◽  
Alejandro H. Corvalán ◽  
Francisco Aguayo
Keyword(s):  
High Risk ◽  
Head And Neck ◽  
Tobacco Smoke ◽  
Epstein Barr Virus ◽  
Potential Role ◽  
Human Papillomaviruses ◽  
Oral Epithelium ◽  
Oncogenic Viruses ◽  
Barr Virus ◽  
Epstein Barr

High-risk human papillomaviruses (HR-HPVs) and Epstein–Barr virus (EBV) are recognized oncogenic viruses involved in the development of a subset of head and neck cancers (HNCs). HR-HPVs are etiologically associated with a subset of oropharyngeal carcinomas (OPCs), whereas EBV is a recognized etiological agent of undifferentiated nasopharyngeal carcinomas (NPCs). In this review, we address epidemiological and mechanistic evidence regarding a potential cooperation between HR-HPV and EBV for HNC development. Considering that: (1) both HR-HPV and EBV infections require cofactors for carcinogenesis; and (2) both oropharyngeal and oral epithelium can be directly exposed to carcinogens, such as alcohol or tobacco smoke, we hypothesize possible interaction mechanisms. The epidemiological and experimental evidence suggests that HR-HPV/EBV cooperation for developing a subset of HNCs is plausible and warrants further investigation.

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