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An Efficient Statistic for Determining the Diameter of Thin Sectioned Uniform Spheres from Measurements of Biological Samples

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050585 ◽

1974 ◽

Vol 32 ◽

pp. 114-115

Author(s):

W. R. Schucany ◽

G. H. Kelsoe ◽

V. F. Allison

Keyword(s):

Biological Samples ◽

Traditional Method ◽

Cell Types ◽

Accurate Estimation ◽

Morphological Identification ◽

Sectioned Material ◽

Maximal Diameter ◽

Similar Cell

Accurate estimation of the size of spheroid organelles from thin sectioned material is often necessary, as uniquely homogenous populations of organelles such as vessicles, granules, or nuclei often are critically important in the morphological identification of similar cell types. However, the difficulty in obtaining accurate diameter measurements of thin sectioned organelles is well known. This difficulty is due to the extreme tenuity of the sectioned material as compared to the size of the intact organelle. In populations where low variance is suspected the traditional method of diameter estimation has been to measure literally hundreds of profiles and to describe the “largest” as representative of the “approximate maximal diameter”.

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Directional observation on lipid of male nectary of Lindera megaphylla hemsl. by the rapid embedding method with polyethylene glycol (PEG)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161151 ◽

1990 ◽

Vol 48 (3) ◽

pp. 718-719

Author(s):

Dai Dalin ◽

Guo Jianmin

Keyword(s):

Electron Microscope ◽

Polyethylene Glycol ◽

Chemical Reaction ◽

Traditional Method ◽

Osmium Tetroxide ◽

Unsaturated Lipid ◽

Embedding Method ◽

Long Period ◽

New Methods

Lipid cytochemistry has not yet advanced far at the EM level. A major problem has been the loss of lipid during dehydration and embedding. Although the adoption of glutaraldehyde and osmium tetroxide accelerate the chemical reaction of lipid and osmium tetroxide can react on the double bouds of unsaturated lipid to from the osmium black, osmium tetroxide can be reduced in saturated lipid and subsequently some of unsaturated lipid are lost during dehydration. In order to reduce the loss of lipid by traditional method, some researchers adopted a few new methods, such as the change of embedding procedure and the adoption of new embedding media, to solve the problem. In a sense, these new methods are effective. They, however, usually require a long period of preparation. In this paper, we do research on the fiora nectary strucure of lauraceae by the rapid-embedding method wwith PEG under electron microscope and attempt to find a better method to solve the problem mentioned above.

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