scholarly journals Evaluation of Immunogenicity, Protective Immunity on Aquaculture Pathogenic Vibrio and Fermentation of Vibrio alginolyticus Flagellin FlaC Protein

2019 ◽  
Vol 17 (3) ◽  
pp. 35-42
Keyword(s):  
Protective Immunity ◽  
Vibrio Alginolyticus ◽  
Pathogenic Vibrio

In Vitro Selection of Oligonucleotide Acid Aptamers against Pathogenic Vibrio Alginolyticus by SELEX

2010 ◽  
Vol 439-440 ◽  
pp. 1456-1462 ◽  
Author(s):  
Jiang Zheng ◽  
Yu Bao Li ◽  
Jia Xiang Li ◽  
Jun Wang ◽  
Yong Quan Su
Keyword(s):  
Secondary Structures ◽  
Magnetic Bead ◽  
Vibrio Alginolyticus ◽  
Pathogenic Microorganism ◽  
Asymmetric Pcr ◽  
High Affinity ◽  
Amplification Method ◽  
Pathogenic Vibrio ◽  
Selection Of

Detection of pathogenic microorganism is very necessary in the control of infectious disease prevailing in aquiculture animals. However, most of the present techniques can not meet the need of the quick field inspection. Systematic evolution of ligands by exponential enrichment (SELEX) is a new molecular recognition way for generating high affinity oligonucleotide acid aptamers, a new nucleotide acid material, which have been widely used in the detections of proteins, cells and so on. In the present paper, the technology was applied to select the high affinity aptamers against pathogenic microorganism Vibrio alginolyticus, which could be used for the rapid field detection of the microorganism. Based on the designment of the ssDNA library of 76 nucleotide acids with 35-base random region, the SELEX system for the selection of the high affinity aptamers against Vibrio alginolyticus was established. In the SELEX system, asymmetric PCR was proved to be a better amplification method for the ssDNA library than the reported affinity magnetic bead method, and the corresponding parameters of the asymmetric PCR were also studied and optimized. The affinity of the final ssDNA library increased by nearly 200% compared with the original library. Cloning and sequencing of the final ssDNA library showed that there were at least two kinds of ssDNAs with different length in the affinity ssDNA library: one was 76 bases, another was 149 bases. Simulation of the secondary structures showed that the secondary structures of the two fragments were different greatly, suggesting that the two fragments could bind to different sites of V. alginolyticus surface.

Molecular characterization and phylogenetic analysis of highly pathogenic Vibrio alginolyticus strains isolated during mortality outbreaks in cultured Ruditapes decussatus juvenile

2017 ◽  
Vol 111 ◽  
pp. 487-496 ◽  
Author(s):  
Badreddine Mechri ◽  
Abir Monastiri ◽  
Amel Medhioub ◽  
Mohamed Nejib Medhioub ◽  
Mahjoub Aouni
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Vibrio Alginolyticus ◽  
Ruditapes Decussatus ◽  
Highly Pathogenic ◽  
Pathogenic Vibrio

Isolation and genotyping of potentially pathogenic Vibrio alginolyticus associated with Ruditapes decussatus larva and juvenile mass mortalities

2014 ◽  
Vol 23 (4) ◽  
pp. 1033-1047 ◽  
Author(s):  
Badreddine Mechri ◽  
Imen Ben Salem ◽  
Amel Medhioub ◽  
Mohamed Nejib Medhioub ◽  
Mahjoub Aouni
Keyword(s):  
Vibrio Alginolyticus ◽  
Ruditapes Decussatus ◽  
Pathogenic Vibrio ◽  
Mass Mortalities ◽  
Larva And Juvenile

Chemotaxis of Pathogenic Vibrio Strains towards Mucus Surfaces of Gilt-Head Sea Bream (Sparus aurataL.)

1998 ◽  
Vol 64 (4) ◽  
pp. 1573-1575 ◽  
Author(s):  
M. Angeles Bordas ◽  
M. Carmen Balebona ◽  
Jose M. Rodriguez-Maroto ◽  
Juan J. Borrego ◽  
Miguel A. Moriñigo
Keyword(s):  
Vibrio Anguillarum ◽  
Bacterial Chemotaxis ◽  
Vibrio Alginolyticus ◽  
Quadratic Polynomial ◽  
Sea Bream ◽  
Influence Of Temperature ◽  
Intestinal Mucus ◽  
Pathogenic Vibrio ◽  
Polynomial Models

ABSTRACT Vibrio anguillarum and Vibrio alginolyticusexhibited significant adhesion to and chemotactic abilities towards mucus collected from the skin, gills, and intestine of gilt-head sea bream. Quadratic polynomial models for chemotaxis designed to estimate the influence of temperature demonstrated a differential bacterial chemotaxis depending of the source of the mucus, with the chemotaxis towards intestinal mucus being the least influenced.

Characteristics of adherence of pathogenic Vibrio alginolyticus to the intestinal mucus of large yellow croaker (Pseudosciaena crocea)

Aquaculture ◽  
2007 ◽  
Vol 269 (1-4) ◽  
pp. 21-30 ◽  
Author(s):  
Qingpi Yan ◽  
Qiang Chen ◽  
Shen Ma ◽  
Zhixia Zhuang ◽  
Xiaoru Wang
Keyword(s):  
Vibrio Alginolyticus ◽  
Large Yellow Croaker ◽  
Pseudosciaena Crocea ◽  
Intestinal Mucus ◽  
Pathogenic Vibrio
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