Chemotaxis of Pathogenic Vibrio Strains towards Mucus Surfaces of Gilt-Head Sea Bream (Sparus aurataL.)

1998 ◽  
Vol 64 (4) ◽  
pp. 1573-1575 ◽  
Author(s):  
M. Angeles Bordas ◽  
M. Carmen Balebona ◽  
Jose M. Rodriguez-Maroto ◽  
Juan J. Borrego ◽  
Miguel A. Moriñigo
Keyword(s):  
Vibrio Anguillarum ◽  
Bacterial Chemotaxis ◽  
Vibrio Alginolyticus ◽  
Quadratic Polynomial ◽  
Sea Bream ◽  
Influence Of Temperature ◽  
Intestinal Mucus ◽  
Pathogenic Vibrio ◽  
Polynomial Models

ABSTRACT Vibrio anguillarum and Vibrio alginolyticusexhibited significant adhesion to and chemotactic abilities towards mucus collected from the skin, gills, and intestine of gilt-head sea bream. Quadratic polynomial models for chemotaxis designed to estimate the influence of temperature demonstrated a differential bacterial chemotaxis depending of the source of the mucus, with the chemotaxis towards intestinal mucus being the least influenced.

In Vitro Selection of Oligonucleotide Acid Aptamers against Pathogenic Vibrio Alginolyticus by SELEX

2010 ◽  
Vol 439-440 ◽  
pp. 1456-1462 ◽  
Author(s):  
Jiang Zheng ◽  
Yu Bao Li ◽  
Jia Xiang Li ◽  
Jun Wang ◽  
Yong Quan Su
Keyword(s):  
Secondary Structures ◽  
Magnetic Bead ◽  
Vibrio Alginolyticus ◽  
Pathogenic Microorganism ◽  
Asymmetric Pcr ◽  
High Affinity ◽  
Amplification Method ◽  
Pathogenic Vibrio ◽  
Selection Of

Detection of pathogenic microorganism is very necessary in the control of infectious disease prevailing in aquiculture animals. However, most of the present techniques can not meet the need of the quick field inspection. Systematic evolution of ligands by exponential enrichment (SELEX) is a new molecular recognition way for generating high affinity oligonucleotide acid aptamers, a new nucleotide acid material, which have been widely used in the detections of proteins, cells and so on. In the present paper, the technology was applied to select the high affinity aptamers against pathogenic microorganism Vibrio alginolyticus, which could be used for the rapid field detection of the microorganism. Based on the designment of the ssDNA library of 76 nucleotide acids with 35-base random region, the SELEX system for the selection of the high affinity aptamers against Vibrio alginolyticus was established. In the SELEX system, asymmetric PCR was proved to be a better amplification method for the ssDNA library than the reported affinity magnetic bead method, and the corresponding parameters of the asymmetric PCR were also studied and optimized. The affinity of the final ssDNA library increased by nearly 200% compared with the original library. Cloning and sequencing of the final ssDNA library showed that there were at least two kinds of ssDNAs with different length in the affinity ssDNA library: one was 76 bases, another was 149 bases. Simulation of the secondary structures showed that the secondary structures of the two fragments were different greatly, suggesting that the two fragments could bind to different sites of V. alginolyticus surface.

Pathogenic Vibrios in Confinement-Reared and Feral Fishes of the Maine–New Hampshire Coast

1978 ◽  
Vol 35 (4) ◽  
pp. 403-408 ◽  
Author(s):  
R. G. Strout ◽  
E. S. Sawyer ◽  
B. A. Coutermarsh
Keyword(s):  
New Hampshire ◽  
West Coast ◽  
Coho Salmon ◽  
Vibrio Anguillarum ◽  
Pseudopleuronectes Americanus ◽  
Agar Gel ◽  
Pathogenic Vibrio ◽  
Test Fish ◽  
Pathogenic Vibrios ◽  
Gel Diffusion

Vibrio anguillarum was isolated from moribund or fresh dead confinement-reared or feral fishes from the Maine–New Hampshire coast. All Vibrio isolates were tested for pathogenicity by inoculation into 12–15 cm-coho salmon (Oncorhynchus kisutch) smolts reared in fresh water. In 1975, of 35 isolates from confinement-reared fishes, 29 were Vibrio and 22 of these killed test fish; 1 of 4 isolates from feral fishes was a pathogenic Vibrio. In 1976, of 69 isolates from cultured fishes, 52 were Vibrio and 12 were pathogenic; 39 of 59 isolates from feral fishes were Vibrio, yet only 1 was pathogenic. One group of Vibrio isolates, all from winter flounder (Pseudopleuronectes americanus), killed all salmon smolts the same day of injection; one strain (569) consistently killed smolts within 3–4 h after inoculation. With microtiter methods, agglutinin titers of rabbit antisera were determined against all pathogenic V. anguillarum isolates. Three distinct antigenic groups of V. anguillarum, confirmed by agar-gel diffusion and challenge, were found on the Maine–New Hampshire coast. Although two of these groups appear to be antigenically similar to West Coast strains 775 and 1669, a third group shows little relationship to other East Coast–West Coast serotypes. Key words: Vibrio anguillarum, coho salmon, pathogenic Vibrio, confinement-reared (cultured) fishes, serotypes, agar-gel diffusion

Neurotoxic effect on two fish species and a PC12 cell line of the supernate of Vibrio alginolyticus and Vibrio anguillarum

1998 ◽  
Vol 63 (1) ◽  
pp. 61-69 ◽  
Author(s):  
M.C Balebona ◽  
K Krovacek ◽  
M.A Moriñigo ◽  
I Mansson ◽  
A Faris ◽  
...  
Keyword(s):  
Cell Line ◽  
Fish Species ◽  
Pc12 Cell ◽  
Vibrio Anguillarum ◽  
Vibrio Alginolyticus ◽  
Neurotoxic Effect ◽  
Pc12 Cell Line
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