scholarly journals HEPATITIS C VIRUS;

2017 ◽  
Vol 24 (11) ◽  
pp. 1621-1629
Author(s):  
Ghulam Mustafa Jamali ◽  
Anwar Ali Jamali ◽  
Habibullah Shaikh
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Real Time ◽  
Virus Disease ◽  
Polymerase Chain Reaction Analysis ◽  
Assay Method ◽  
Virus Infectivity ◽  
Chain Reaction ◽  
Polymerase Chain

Objectives: The plan of this current research was in the direction for towards theassessment of the existing ELISA (Enzyme Linked Immunosorbant Assay) method throughantibodies testing for identification of hepatitis C virus disease by comparing their outcome withthe Real Time polymerase chain reaction analysis. Setting: Peoples Medical College HospitalNawabshah. Period: December 2015 to December 2016. Methods: In this current research 100blood samples were analyzed due to the presence of anti-HCV antibodies by 3rd-generationenzyme-linked immunosorbent assay testing. All the specimens were 100% positive. Polymerasechain reaction test was performed according to the laboratory directions in anti- hepatitis C virusantibodies positive patients to validate the diagnosis of hepatitis C virus infectivity. Results: Thisresearch shows that, the entire results were positive by Enzyme Linked Immunosorbant Assaytesting. As compared with polymerase chain reaction the of Enzyme Linked ImmunosorbantAssay in this research the screening test for anti hepatitis C virus - antibodies is about 2%false positive. Out of the 100 samples 98 cases are positive by Real Time polymerase chainreaction analysis while only 02 cases report are negative (2%). Conclusion: The proportion ofhepatitis C virus infectivity was 100% by 3rd-generation enzyme-linked immunosorbent assaytesting, 98% by Real Time polymerase chain reaction analysis. As in our research the hepatitisC virus –Ribonucleic acid is present in 98% cases who are the Anti- hepatitis C virus antibodiespositive patients, it can be suggested that Anti-HCV antibodies detection by third generationELISA technique in routine procedure is sufficient to determine HCV infection.

scholarly journals Overestimation and underestimation of hepatitis C virus RNA levels in a widely used real-time polymerase chain reaction-based method

Hepatology ◽  
2007 ◽  
Vol 46 (1) ◽  
pp. 22-31 ◽  
Author(s):  
Stéphane Chevaliez ◽  
Magali Bouvier-Alias ◽  
Rozenn Brillet ◽  
Jean-Michel Pawlotsky
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Real Time ◽  
Hepatitis C Virus Rna ◽  
Chain Reaction ◽  
Virus Rna ◽  
Polymerase Chain ◽  
Rna Levels

scholarly journals Frequency of Hepatitis C Virus in Diagnosed Tuberculosis Cases

2021 ◽  
Vol 15 (5) ◽  
pp. 1041-1043
Author(s):  
Muhammad Farooq Baig ◽  
Geeta Kumari ◽  
Amir Bux Detho ◽  
Abdul Saleem Qadeer ◽  
Ball Chand ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Immunosorbent Assay ◽  
Rapid Test ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hepatitis C Virus Infection ◽  
Chain Reaction ◽  
Polymerase Chain

Background: The frequency of hepatitis C virus infection along with tuberculosis has not been widely investigated and very low statistics on rates of hepatitis C virus co-infection in tuberculosis patients. Hepatotoxicity is the major side effect of anti-tuberculosis therapy hepatitis HCVliver disease elevates the chances of hepatotoxicity up-to five folds. Aim: To see the frequency of Hepatitis Cvirus infection amongst people with diagnosed Tuberculosis using gene X-pert technique. To evaluate the factors associated with HCVinfection in patients with MTBtuberculosis and to determine sensitivity and specificity of the tests. Study design: Comparative analytical study. Place and duration of study: Pathology Department, Civil Hospital Mirpur Khas Sindh from 1stJanuary 2017 to 31st December 2018. Methodology: Three hundred and thirteen patients of tuberculosis diagnosed by Genexpert included while testing hepatitis C virus using immunochromotography rapid test technique, enzyme linked immunosorbent assay method and polymerase chain reaction test for confirmation. Results:Higher frequency of tuberculosis infection in males 57.8%, 42.5% between 20-39 years and 22% of hepatitis C virus infection in tuberculosis patients.Sensitivity of rapid test and enzyme-linked immunosorbent assay was 79% and 96% respectively while the specificity of rapid test and enzyme-linked immunosorbent assay was 91% and 99% respectively. Conclusion: The high frequency of hepatitis C virus co-infection was found among tuberculosis cases in Mirpur Khas Division Sindh. Enzyme-linked immunosorbent assay method is more accurate, reliable as compared to rapid immunochromatographytest for hepatitis C virus and polymerase chain reaction is still gold standard. Keywords: TB, Hepatitis C virus, Mycobacterium tuberculosis,, PCR, Genexpert, Rapid test

A simple real-time polymerase chain reaction assay using SYBR Green for hepatitis C virus genotyping

2016 ◽  
Vol 162 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Hosam Zaghloul ◽  
A. A. El Morsi ◽  
H. E. Soweha ◽  
Ashraf Elsayed ◽  
S. Seif ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Real Time ◽  
Polymerase Chain Reaction Assay ◽  
Sybr Green ◽  
Chain Reaction ◽  
Polymerase Chain
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