scholarly journals Influence of Pollen Germination Capacity on a Successful Artificial Hybridization in Cyclamen sp

2019 ◽  
Vol 3 (1) ◽  
pp. 53-60
Author(s):  
Mirela Irina Cordea ◽  
◽  
Alexandra Tiriplică ◽  
Keyword(s):  
Pollen Germination ◽  
Germination Capacity ◽  
Artificial Hybridization

scholarly journals The effect of pollen performance on low seed fertility in a Greek population of Juniperus excelsa

2019 ◽  
Vol 65 (No. 9) ◽  
pp. 356-367 ◽  
Author(s):  
Eleftherios Karapatzak ◽  
Georgios Varsamis ◽  
Irene Koutseri ◽  
Ioannis Takos ◽  
Theodora Merou
Keyword(s):  
Embryo Development ◽  
Natural Regeneration ◽  
Pollen Germination ◽  
Tree Height ◽  
Germination Capacity ◽  
Northern Greece ◽  
Physiological Basis ◽  
Seed Fertility ◽  
Juniperus Excelsa

The Juniperus excelsa (Cupressaceae) forest in northern Greece has been facing a documented severe decline of natural regeneration over the last few years. In order to dissect the physiological components of the low regeneration, pollen originated from different sites as well as from trees of different height was investigated in terms of viability and in vitro germination capacity. In addition, embryo development and viability of the first year immature seeds were further assessed. Pollen was found to present high viability (61.5–93.2%) in all sites and tree height classes. However, pollen germination was quite low (maximum germination percentage recorded was 30.2%). The low pollen germination capacity recorded in trees above 6 m in height was reflected in the subsequent low percentages of successful embryo development and viability found in the produced seeds. These results suggest that low seed fertility in Juniperus excelsa is associated with reduced pollen germination, suggesting a complex regeneration mechanism and is considered an additional step in shedding light on the physiological basis of the low natural regeneration of Juniperus excelsa.

scholarly journals Irradiation and Heat Affect Peach Pollen Germination and Fertility

HortScience ◽  
1997 ◽  
Vol 32 (2) ◽  
pp. 290-291 ◽  
Author(s):  
M.R. Pooler ◽  
R. Scorza
Keyword(s):  
Pollen Germination ◽  
Interspecific Hybrids ◽  
Prunus Persica ◽  
Seed Set ◽  
Germination Capacity ◽  
Male Sterile ◽  
In Vitro Pollen Germination ◽  
Low Levels ◽  
Parthenogenetic Egg

Pollen from the doubled haploid peach [Prunus persica (L.) Batsch] `Hall-D' was irradiated with 0, 290, 530, 820, 1000, 5000, or 9000 Gray (Gy) of gamma radiation, 113 μW·cm-2 of ultraviolet (UV) radiation, or exposed to 100 °C for 2 h. In vitro pollen germination percentages were recorded and pollen was used to pollinate more than 10,000 emasculated or male-sterile peach flowers. Although pollen germination in vitro was stimulated by <1000 Gy of gamma irradiation, seed set following pollination was greatly reduced in all treatments. These results suggest that low levels of irradiation are sufficient to render pollen infertile while still maintaining germination capacity. Such results may be useful for pollination-induced parthenogenetic egg division for the production of maternally derived haploids and for the production of interspecific hybrids.

scholarly journals Influence of Technology and Environment on the Pollen Germination Capacity in Cherry

2018 ◽  
Vol 75 (2) ◽  
Author(s):  
Andreea Flavia ANDRECAN ◽  
Viorel MITRE ◽  
Olimpia IORDĂNESCU ◽  
Mirela Irina CORDEA
Keyword(s):  
Environmental Conditions ◽  
Pollen Fertility ◽  
Pollen Germination ◽  
Germination Percentage ◽  
Maximum Temperature ◽  
Germination Capacity ◽  
Bud Formation ◽  
High Maximum

Pollen of four cherry cultivars (‘Lapins, ‘Kordia’, ‘Sylvia’, ‘Regina’) was collected and examined with microscop to determine its germination capacity. It was observed that pollen fertility varied from 18.7 % to 80.1 %. Environmental conditions of the studied area as well as applied treatments affected pollen germination capacity. Due to the high maximum temperature recorded in 13.03.2018, bud formation was induced 18 day earlier than in the previous years, growth started on March 31st, when the temperature was 21.5˚C. Regarding production, the highest yield was obtained in ‘Regina’ variety 9.7 kg/tree, ‘Kordia’ 9.3 kg/tree, ‘Lapins’ 8.8 kg/tree and Sylvia 8.2 kg/tree. In autumn of the previous year, the trees were fertilized with urea, in a concentration of 3 kg per ha, and in the spring with CROPAID, in a concentration of 5 liters per ha. The results show that germination percentage was much higher in the pollen collected from the treated trees than in the untreated ones.

Changes in polyamine synthesis during anther development and pollen germination in tobacco (Nicotiana tabacum)

1994 ◽  
Vol 92 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Fatiha Chibi ◽  
Angel Jesus Matilla ◽  
Trinidad Angosto ◽  
Dolores Garrido
Keyword(s):  
Nicotiana Tabacum ◽  
Pollen Germination ◽  
Anther Development ◽  
Polyamine Synthesis

Artificial Hybridization of Ganoderma lucidum and G. tsugae Murrill by Protoplast Fusion for Sustainability

2005 ◽  
Vol 7 (1-2) ◽  
pp. 263-280 ◽  
Author(s):  
Siu Wai Chiu ◽  
Vivien Wing Yan Luk ◽  
Stephen Yu ◽  
Peggy Lee ◽  
Natalie Wai ◽  
...  
Keyword(s):  
Protoplast Fusion ◽  
Ganoderma Lucidum ◽  
Artificial Hybridization

scholarly journals Pollen Germination in Different Genotypes of Gladiolus

1970 ◽  
Vol 11 ◽  
pp. 47-50
Author(s):  
Tul Bahadur Poon ◽  
TM Rao ◽  
C Aswath ◽  
PE Rajasekharan ◽  
DP Kumar
Keyword(s):  
Boric Acid ◽  
Magnesium Sulphate ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Calcium Nitrate ◽  
Potassium Nitrate ◽  
Hybrid Selection ◽  
Fresh Pollen ◽  
Sterile Pollen ◽  
Germinated Pollen

Fresh pollen of 16 promising genotypes of gladiolus was tested for their pollen viability. Modified cellophane method was employed to assess the pollen viability. Pollen germination media consisted of 15% sucrose supplemented with 300 ppm calcium nitrate, 200 ppm magnesium sulphate, 100 ppm potassium nitrate and 100 ppm boric acid. Highly significant variations were observed for percentage of pollen germination, non-germinated pollen and sterile pollen. The highest pollen germination (76.41%) was in genotype Hybrid selection 88-10-22, and did not differ significantly from Gladiolus callianthus (75.41%), Sapna (75.10 %), Hybrid selection 86-32-11, (73.28 %), Kum Kum (69.41%), Poonam (69.22 %), Hybrid selection 87-22-1 (67.87 %), Hybrid selection 87-1-1 (67.61 %), Psittacinus hybrid (64.64%) and Darshan (63.97%). The lowest non- germinated pollen (10.47%) was in genotype Gladiolus callianthus, and insignificantly followed by Hybrid selection 88-10-22 with 18.77 % and Hybrid selection 87-22-1 with 18.95 %. The lowest percentage of sterile pollen was noticed in genotype Sapna (2.82%) followed by Poonam with 4.00 % Hybrid selection 88-10-22 with 4.82% and Hybrid selection 82-11-27 with 5.22%. Key words: Gladiolus; germination; pollen DOI: 10.3126/njst.v11i0.4089Nepal Journal of Science and Technology 11 (2010) 47-50

scholarly journals Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin-depolymerizing factor 1 on microfilaments in okra

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Biying Dong ◽  
Qing Yang ◽  
Zhihua Song ◽  
Lili Niu ◽  
Hongyan Cao ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Actin Depolymerizing Factor ◽  
Specific Mechanism ◽  
Promoting Effect ◽  
New Research

AbstractMature pollen germinates rapidly on the stigma, extending its pollen tube to deliver sperm cells to the ovule for fertilization. The success of this process is an important factor that limits output. The flavonoid content increased significantly during pollen germination and pollen tube growth, which suggests it may play an important role in these processes. However, the specific mechanism of this involvement has been little researched. Our previous research found that hyperoside can prolong the flowering period of Abelmoschus esculentus (okra), but its specific mechanism is still unclear. Therefore, in this study, we focused on the effect of hyperoside in regulating the actin-depolymerizing factor (ADF), which further affects the germination and growth of pollen. We found that hyperoside can prolong the effective pollination period of okra by 2–3-fold and promote the growth of pollen tubes in the style. Then, we used Nicotiana benthamiana cells as a research system and found that hyperoside accelerates the depolymerization of intercellular microfilaments. Hyperoside can promote pollen germination and pollen tube elongation in vitro. Moreover, AeADF1 was identified out of all AeADF genes as being highly expressed in pollen tubes in response to hyperoside. In addition, hyperoside promoted AeADF1-mediated microfilament dissipation according to microfilament severing experiments in vitro. In the pollen tube, the gene expression of AeADF1 was reduced to 1/5 by oligonucleotide transfection. The decrease in the expression level of AeADF1 partially reduced the promoting effect of hyperoside on pollen germination and pollen tube growth. This research provides new research directions for flavonoids in reproductive development.

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