THE ROLE OF LYSOSOMAL ENZYMES IN THE PATHOGENESIS OF SHOCK. 2, THE ORIGIN OF PLASMA LYSOSOMAL HYDROLASES IN SHOCK

Neutrophil infiltration plays a major role in the pathogenesis of myocardial injury. Oxidative injury is suggested to be a central mechanism of the cellular damage after acute myocardial infarction. This study is pertained to the prognostic role of a tetrapeptide derivative PEP1261 (BOC-Lys(BOC)-Arg-Asp-Ser(tBu)-OtBU), a peptide sequence (39–42) of lactoferrin, studied in the modulation of neutrophil functions in vitro by measuring the reactive oxygen species (ROS) generation, lysosomal enzymes release, and enhanced expression of C proteins. The groundwork experimentation was concerned with the isolation of neutrophils from the normal and acute myocardial infarct rats to find out the efficacy of PEP1261 in the presence of a powerful neutrophil stimulant, phorbol 12-myristate 13 acetate (PMA). Stimulation of neutrophils with PMA resulted in an oxidative burst of superoxide anion and enhanced release of lysosomal enzymes and expression of complement proteins. The present study further demonstrated that the free radicals increase the complement factors in the neutrophils confirming the role of ROS. PEP1261 treatment significantly reduced the levels of superoxide anion and inhibited the release of lysosomal enzymes in the stimulated control and infarct rat neutrophils. This study demonstrated that PEP1261 significantly inhibited the effect on the ROS generation as well as the mRNA synthesis and expression of the complement factors in neutrophils isolated from infarct heart.

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Fructose 1,6-Bisphosphatase: The Role of Lysosomal Enzymes in the Modification of Catalytic and Structural Properties

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.70.2.303 ◽

1973 ◽

Vol 70 (2) ◽

pp. 303-305 ◽

Cited By ~ 18

Author(s):

S. Pontremoli ◽

E. Melloni ◽

F. Balestrero ◽

A. T. Franzi ◽

A. De Flora ◽

...

Keyword(s):

Structural Properties ◽

Lysosomal Enzymes ◽

Fructose 1,6 Bisphosphatase

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The Role of Lysosomal Hydrolases in the Mechanism of Shock

Advances in Experimental Medicine and Biology - Neurohumoral and Metabolic Aspects of Injury ◽

10.1007/978-1-4684-3228-2_56 ◽

1973 ◽

pp. 535-543 ◽

Cited By ~ 3

Author(s):

James J. Smith ◽

Daniel J. Loegering ◽

Daniel J. McDermott ◽

Michael L. Bonin

Keyword(s):

Lysosomal Hydrolases

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The role of lysosomal sialidase and ?-galactosidase in processing the complex carbohydrate chains on lysosomal enzymes and possibly other glycoproteins

Annals of Human Genetics ◽

10.1111/j.1469-1809.1984.tb01017.x ◽

1984 ◽

Vol 48 (3) ◽

pp. 215-221 ◽

Cited By ~ 5

Author(s):

D. M. SWALLOW ◽

L. F. WEST ◽

A. VAN ELSEN

Keyword(s):

Lysosomal Enzymes ◽

Complex Carbohydrate ◽

Carbohydrate Chains ◽

Lysosomal Sialidase

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The emerging role of mast cell proteases in asthma

European Respiratory Journal ◽

10.1183/13993003.00685-2019 ◽

2019 ◽

Vol 54 (4) ◽

pp. 1900685 ◽

Cited By ~ 4

Author(s):

Gunnar Pejler

Keyword(s):

Mast Cell ◽

Mast Cells ◽

Current Knowledge ◽

Secretory Granules ◽

Cross Linking ◽

Lysosomal Hydrolases ◽

Ige Receptor ◽

Deficient Mice ◽

Lines Of Evidence

It is now well established that mast cells (MCs) play a crucial role in asthma. This is supported by multiple lines of evidence, including both clinical studies and studies on MC-deficient mice. However, there is still only limited knowledge of the exact effector mechanism(s) by which MCs influence asthma pathology. MCs contain large amounts of secretory granules, which are filled with a variety of bioactive compounds including histamine, cytokines, lysosomal hydrolases, serglycin proteoglycans and a number of MC-restricted proteases. When MCs are activated, e.g. in response to IgE receptor cross-linking, the contents of their granules are released to the exterior and can cause a massive inflammatory reaction. The MC-restricted proteases include tryptases, chymases and carboxypeptidase A3, and these are expressed and stored at remarkably high levels. There is now emerging evidence supporting a prominent role of these enzymes in the pathology of asthma. Interestingly, however, the role of the MC-restricted proteases is multifaceted, encompassing both protective and detrimental activities. Here, the current knowledge of how the MC-restricted proteases impact on asthma is reviewed.

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A study of kidney lysosomal acid phosphatase during compensatory renal hyperplasia in rats

Canadian Journal of Zoology ◽

10.1139/z68-069 ◽

1968 ◽

Vol 46 (3) ◽

pp. 499-502 ◽

Cited By ~ 2

Author(s):

B. M. Hegdekar

Keyword(s):

Acid Phosphatase ◽

Lysosomal Enzymes ◽

Soluble Fraction ◽

Female Rats ◽

Enzyme Release ◽

Lysosomal Fraction ◽

Probable Role ◽

Long Evans ◽

Free Activity

Female rats of the Long-Evans hooded strain, 4–6 months old and weighing 275–300 grams, were subjected to unilateral nephrectomy and the acid phosphatase activity in the remaining kidney was studied at the end of 24, 48, 72 hours, and 8 days respectively. Most of the acid phosphatase was found in the particulate fraction in normal kidneys. The enzyme activity in the soluble fraction was found to have increased the second day after the operation, but decreased to the original level by the end of 72 hours. The free activity of the lysosomal fraction also increased by the end of second postoperative day. A change in the permeability of the lysosomal membrane before the enzyme release was observed. The probable role of lysosomal enzymes in the initiation of mitotic divisions during compensatory renal hyperplasia is discussed.

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