Morphological identification of fungi associated with Eichhornia crassipes (Mart.-Solms) Laubach in the Wouri River Basin, Douala, Cameroon

2019 ◽  
Vol 44 (3) ◽  
pp. 195-208 ◽  
Author(s):  
SN Kenfack Voukeng ◽  
C Coombes ◽  
P Weyl ◽  
F Djeugoue ◽  
MP Hill
Keyword(s):  
River Basin ◽  
Eichhornia Crassipes ◽  
Morphological Identification ◽  
Identification Of Fungi

scholarly journals The Water Hyacinth, Eichhornia crassipes: an invasive plant in the Guadiana River Basin (Spain)

2008 ◽  
Vol 3 (1) ◽  
pp. 42-53 ◽  
Author(s):  
Trinidad Ruiz Téllez ◽  
Elsa López ◽  
Gloria Granado ◽  
Eva Pérez ◽  
Ricardo López ◽  
...  
Keyword(s):  
River Basin ◽  
Water Hyacinth ◽  
Eichhornia Crassipes ◽  
Invasive Plant ◽  
Guadiana River Basin

scholarly journals Morphological and Molecular Identification of Fungi Causing Canker Disease on Melia Azidarch Trees in Some Regions of Mosul Provinces, Iraq

2021 ◽  
Vol 910 (1) ◽  
pp. 012116
Author(s):  
Anwer Noori Alkhero ◽  
Zainab Waadallah Rassem
Keyword(s):  
Molecular Diagnosis ◽  
Molecular Identification ◽  
First Record ◽  
Alternaria Brassicicola ◽  
Morphological Identification ◽  
University Campus ◽  
Canker Disease ◽  
Identification Of Fungi ◽  
Nattrassia Mangiferae ◽  
High Pathogenic

Abstract The aim of this study was to identify the fungi associated with canker disease on Melia azidarch trees inside Mosul University campus and the presidential palaces regions in Mosul Province, Iraq. Results of isolation showed the presenting of the fungi (Nattrassia mangiferae, Neoscylitidium dimidiatum Penz., Fusarium graminearium Schw., Alternaria brassicicola Schw., Aspergillus sp. and Penecillium sp.), which accompanied with the samples displayed cankering symptoms during the period from April to December/2020, the maximum of dominance was 85% for the fungus Neoscylitidium dimidiatum in August, while the lowest was 49% in April for the same year, followed by Fusarium graminearium with 38% in December, while the lowest percentage was 4% in October, then Alternaria brassicicola Schw. was 25% in April and the lowest value was 0% in August, followed by Aspergillus sp. and Penecillium sp. with low isolation percentages the maximum of which 25% and the lowest is 0% in August. When studying the pathogenicity of the isolated fungi, the results showed a high pathogenic effect in terms the length, diameter and the area of cankers symptom. Based on the results of the molecular diagnosis, the morphological identification was confirmed and it was clear that Fusarium austroamericanum, detection is considered the first record of this fungus in Iraq Melia azidarch trees.

Molecular and morphological identification of fungi isolated from sour cherry, plum and apple fruit leather in Iran

Gene Reports ◽  
2019 ◽  
Vol 17 ◽  
pp. 100500
Author(s):  
Atefeh Mehraban ◽  
Reza Nasr ◽  
Majid Eslami ◽  
Hadi Amrollahi
Keyword(s):  
Sour Cherry ◽  
Apple Fruit ◽  
Morphological Identification ◽  
Identification Of Fungi

scholarly journals Evaluation of Mansonia spp. Infestation on Aquatic Plants in Lentic and Lotic Environments of the Madeira River Basin in Porto Velho, Rondônia, Brazil

2021 ◽  
Vol 37 (3) ◽  
pp. 143-151
Author(s):  
Luiz H. Soares Gil ◽  
Cecilia F. Mello ◽  
Júlia Dos S. Silva ◽  
Juliana Da S. Oliveira ◽  
Shayenne Olsson Freitas Silva ◽  
...  
Keyword(s):  
River Basin ◽  
Aquatic Plants ◽  
Eichhornia Crassipes ◽  
Environmental Changes ◽  
Abiotic Factors ◽  
Larval Density ◽  
Organic Matter Content ◽  
Madeira River ◽  
Lotic Environments ◽  
Madeira River Basin

ABSTRACT The females of Mansonia are voraciously hematophagous. The spiracular apparatus of the immature, larval, and pupal forms is adapted to perforate submerged aquatic vegetation, from whose aeriferous aerenchyma they obtain the oxygen necessary for breathing. The proliferation of aquatic plants, in some cases linked to anthropic modifications that reduce water flow and/or increase organic matter content, may therefore contribute to the spread of these mosquitoes. This study aims to assess the presence of immature individuals of Mansonia in different aquatic plants of the Madeira River basin in 10 lentic and lotic environments and correlate their population density with abiotic factors such as water pH, dissolved O2, conductivity, and temperature. The sampling lasted from February 2016 to June 2018, a 29-month period during which 31,287 specimens belonging to the genus Mansonia were captured. Of the 12 species of macrophytes inspected, Eichhornia crassipes made up 70.1% of the samples. Lentic environments accounted for 58.9% of the samples and lotic environments for 41.1%. Immature individuals were most commonly found on Eichhornia crassipes, with this plant accounting for an average of 96.2% of all individuals, with a percentage ranging between 58.2% and 77.1% in different breeding areas. Only at the Foz do Igarapé Jirau site was a different distribution observed, with the number of aquatic plants more nearly equal: 83.3% of the larvae were found in Eichhornia crassipes, 9.2% in Ceratopteris pteridoides, 3.6% in E. azurea, 2.0% in Salvinia sp., and 1.9% in Pistia sp. The greatest richness was found in Iguapé do Raul. Concerning the larval/plant relationship, although less frequent, E. azurea had a higher larval density of Mansonia spp. It is important to emphasize that this finding may indicate a possible selection for this plant. Egg deposition by Mansonia spp. was more abundant in sample areas with Eichhornia crassipes and Pistia sp. The number of specimens collected was positively correlated with temperature, pH, and conductivity. These correlations showed a marked increase in the rainy season. Therefore, we were able to establish preliminary parameters of how environmental changes influence the ecology of this important genus of mosquitoes, the species of which are critical disease vectors.

scholarly journals Study of Staining Capability of Alcoholic and Aqueous Hibiscus sabdariffa Extract in Demonstration of Selected Fungal Moulds in Some Selected Tissue Section of Wistar Rat

2020 ◽  
pp. 26-33
Author(s):  
S. Y. Ma’aruf ◽  
M. O. Mohammed ◽  
O. G. Avwioro ◽  
A. T. Muhammad ◽  
R. I. Tsamiya ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Tissue Section ◽  
Glacial Acetic Acid ◽  
Ammonium Hydroxide ◽  
Wistar Rat ◽  
Morphological Identification ◽  
Hibiscus Sabdariffa ◽  
Identification Of Fungi ◽  
Formalin Fixed Paraffin Embedded ◽  
Green Background

Introduction: Hibiscus sabdariifa is a natural dye, generally called roselle. Most of Synthetic dye  have been established to cause toxicity. The aim of this research was to determine the staining effect of different concentration of Hibiscus sabdariffa extracts at various pH, duration on fungal staining. Methods: Standard Gomori’s metenaine silver stains as control and Hibiscus sabdariffa extracts (alcoholic and aqueous) were used to stain a fungal (Aspergillus spp) positive lung tissue, using various concentration (5% and 10%) of the extract, with change of pH achieved by treating the extracts with ammonium hydroxide and glacial acetic acid at various duration (30 seconds and 1 hour respectively), each was used for staining Aspergillus spp in tissue section as a primary stain replacing (4% Chromic acid, sodium metabisulphite, Hexamine working silver solution and Sodium thiosulphate). Results: All extracts after treatment were acidic, the fungi were  best demonstrated with Aqueous hibiscus stains (5% untreated, 10% untreated and 5% glacial acetic acid treated) at longer duration, staining the capsule, light to dark-brown in a light-green background when compared with the alcoholic hibiscus stain. Few of the alcoholic hibiscus stains show metachromatic property. The Hibiscus stains stained better at longer duration and the change of pH was indirectly proportional to the staining ability of the hibiscus stains. Conclusion: The results obtained indicate that hibiscus plant extract have the potential for use in the morphological identification of fungi in formalin-fixed paraffin-embedded tissue section.

An Efficient Statistic for Determining the Diameter of Thin Sectioned Uniform Spheres from Measurements of Biological Samples

1974 ◽  
Vol 32 ◽  
pp. 114-115
Author(s):  
W. R. Schucany ◽  
G. H. Kelsoe ◽  
V. F. Allison
Keyword(s):  
Biological Samples ◽  
Traditional Method ◽  
Cell Types ◽  
Accurate Estimation ◽  
Morphological Identification ◽  
Sectioned Material ◽  
Maximal Diameter ◽  
Similar Cell

Accurate estimation of the size of spheroid organelles from thin sectioned material is often necessary, as uniquely homogenous populations of organelles such as vessicles, granules, or nuclei often are critically important in the morphological identification of similar cell types. However, the difficulty in obtaining accurate diameter measurements of thin sectioned organelles is well known. This difficulty is due to the extreme tenuity of the sectioned material as compared to the size of the intact organelle. In populations where low variance is suspected the traditional method of diameter estimation has been to measure literally hundreds of profiles and to describe the “largest” as representative of the “approximate maximal diameter”.

Application of solid-phase immune electron microscopy to study physical and stability characteristics of enterically transmitted non-a, non-b hepatitis virus

1988 ◽  
Vol 46 ◽  
pp. 80-81
Author(s):  
Charles D. Humphrey ◽  
E. H. Cook ◽  
Karen A. McCaustland ◽  
Daniel W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Etiologic Agent ◽  
World Health ◽  
Health Concern ◽  
Morphological Identification ◽  
Immune Electron Microscopy

Enterically transmitted non-A, non-B hepatitis (ET-NANBH) is a type of hepatitis which is increasingly becoming a significant world health concern. As with hepatitis A virus (HAV), spread is by the fecal-oral mode of transmission. Until recently, the etiologic agent had not been isolated and identified. We have succeeded in the isolation and preliminary characterization of this virus and demonstrating that this agent can cause hepatic disease and seroconversion in experimental primates. Our characterization of this virus was facilitated by immune (IEM) and solid phase immune electron microscopic (SPIEM) methodologies.Many immune electron microscopy methodologies have been used for morphological identification and characterization of viruses. We have previously reported a highly effective solid phase immune electron microscopy procedure which facilitated identification of hepatitis A virus (HAV) in crude cell culture extracts. More recently we have reported utilization of the method for identification of an etiologic agent responsible for (ET-NANBH).

Sign in / Sign up

Export Citation Format

Share Document