scholarly journals The Potential of Fungal Isolates from Vermicompost Water Extract to Inhibit Alternaria solani in Vitro and Suppress Early Blight Disease in Tomato

2021 ◽  
Author(s):  
Noor Istifadah ◽  
Retno Anjani Putri ◽  
Fitri Widiantini ◽  
Sri Hartati
Keyword(s):  
Early Blight ◽  
Water Extract ◽  
Alternaria Solani ◽  
Fungal Isolates ◽  
Blight Disease

scholarly journals A Novel Rhizospheric Bacterium, Bacillus Velezensis NKMV-3 as a Biocontrol Agent against Alternaria Leaf Blight in Tomato

2021 ◽  
Author(s):  
Vignesh Murthy ◽  
VedhaHari BodethalaNarayanan ◽  
MubarakAli Davoodbasha ◽  
MadhanShankar ShankarRamakrishanan
Keyword(s):  
Early Blight ◽  
Plant Pathogens ◽  
Alternaria Solani ◽  
Inhibitory Effect ◽  
Rrna Gene ◽  
Bacillus Velezensis ◽  
Bacterium Bacillus ◽  
Blight Disease ◽  
Molecular Sequencing

Abstract A novel strain of Bacillus isolated from rhizosphere has shown to be excellent biocontrol agents against various plant pathogens. In this study, a first report of a Bacillus strain NKMV-3 which effectively controlling Alternaria solani, which cause the Early Blight disease in tomato. Based on the cultural and molecular sequencing of 16S rRNA gene sequence, the identity of the strain was confirmed as Bacillus velezensis NKMV-3. The presence of the lipopeptide which are antibiotic synthesis genes namely Iturin C, Surfactin A, Fengycin B and D were confirmed through gene amplification. In addition, lipopetides was also confirmed through liquid chromatography. The extract showed inhibitory effect against A.solani in-vitro and detached tomato leaf assays. Bacillus velezensis strain NKMV-3 based formulations may provide an effective solution in controlling early blight disease in tomato and other crops.

scholarly journals Curvularia Lunata as New Causal Pathogen of Tomato Early Blight Disease in Egypt

2021 ◽  
Author(s):  
Heba-Alla S. AbdElatah ◽  
Nashwa M.A. Sallam ◽  
Mohamed S. Mohamed ◽  
Hadeel M. M. Khalil Bagy
Keyword(s):  
Early Blight ◽  
Alternaria Solani ◽  
Potato Dextrose Agar ◽  
Curvularia Lunata ◽  
Tomato Cultivar ◽  
Tomato Plants ◽  
Fungal Isolates ◽  
Pathogenicity Tests ◽  
Causal Pathogen ◽  
Blight Disease

Abstract Tomato plants displaying early blight symptoms were collected from different localities in the provinces of Assiut and Sohag, Egypt. The causal pathogens were isolated on potato dextrose agar plates. Pathogenicity tests with 48 isolates were carried out under greenhouse conditions on tomato cultivar (CV 844). All tested isolates caused symptoms of early blight disease with different degrees. The highest disease severity on tomato plants was found after inoculation with isolate No. 6 followed by isolates No. 20 and No. 31. The most pathogenic isolates were identified by sequence analysis using ITS1 and ITS4 primers. The analysis of the amplified sequences from fungal isolates No. 6, 20 and 31 displayed 99 - 100% nucleotide identity with Alternaria solani, Curvularia lunata and A. alternata, respectively. To our knowledge, this is the first report of Curvularia lunata as one of the causal pathogens of early blight disease of tomato plants in Egypt.

scholarly journals In vitro antifungal evaluation of various plant extracts against early blight disease (Alternaria solani) of potato

2011 ◽  
Vol 10 (42) ◽  
pp. 8291-8295 ◽  
Author(s):  
Yanar Yusuf ◽  
Gouml kccedil e Ayhan ◽  
Kadioglu Izzet ◽  
Ccedil am Halit ◽  
Whalon Mark
Keyword(s):  
Plant Extracts ◽  
Early Blight ◽  
Alternaria Solani ◽  
Blight Disease

scholarly journals Efficacy of different fungicides for management of early blight disease of potato

2017 ◽  
Vol 9 (1) ◽  
pp. 280-285
Author(s):  
S. Murmu ◽  
S. Dey ◽  
A. Chakraborty
Keyword(s):  
Early Blight ◽  
Alternaria Solani ◽  
Control Measures ◽  
Antifungal Compounds ◽  
Germination Inhibition ◽  
Disease Reduction ◽  
Blight Disease ◽  
Chemical Fungicides

The early blight of potato may be controlled by using bio-control agents and plant extracts but not effectively and rapidly. But using of chemical fungicides the disease can be controlled easily and losses of yield will be reduced compare to above mentioned both control measures. Keeping this in mind an experiment was conducted to test the efficacy of seven different fungicides (difenoconazole, propiconazole, hexaconazole, propineb 61% + iprov-alicarb 5.25%, propineb, carbendazim and metalaxyl 8% + mancozeb 64%) for controlling early blight of potato un-der in vivo as well as in vitro condition. These antifungal compounds were applied 3 times at 7days interval after first appearance of the disease in the field. Highest percent disease reduction (57.88%) and highest yield of tuber (27.03 t/ha) was recorded in plot sprayed with propiconazole @1 ml/lit followed by propineb and propineb 61% + iprovalicarb 5.25%, with percent disease reduction 55.98%, 51.90% and yield 26.30 t/ha and 24.53 t/ha respectively. Simultaneously in vitro efficacy of these fungicides were also tested against Alternaria solani where propiconazole and propineb exhibited highest percent inhibition(100%) in radial growth and in case of spore germination inhibition in spite of these two fungicides difenoconazole, hexaconazole and propineb 61% + iprovalicarb 5.25% exhibit same result i.e. 100% inhibition compared to control as well as other treatments. Therefore it may be suggested that propiconazole and propineb can be used successfully in controlling of this disease.

scholarly journals Kemampuan Kompos dan Kompos Plus untuk Meningkatkan Ketahanan Tanaman Tomat terhadap Penyakit Bercak Coklat (Alternaria solani Sor.)

Agrikultura ◽  
2017 ◽  
Vol 28 (3) ◽  
Author(s):  
Noor Istifadah ◽  
Nasrul Hakim
Keyword(s):  
Disease Control ◽  
Plant Resistance ◽  
Trichoderma Harzianum ◽  
Early Blight ◽  
Organic Fertilizer ◽  
Water Extract ◽  
Alternaria Solani ◽  
Antagonistic Fungi ◽  
Application Methods ◽  
Blight Disease

ABSTRACTAbilities of compost and compost plus to enhance tomato resistance to early blight (Alternaria solani Sor.)Compost can be used for organic fertilizer and also for disease control. To enhance its effectiveness, compost was fortified with antagonistic microbes and called compost plus. The aims of this study were to examine the potential of compost plus and its component, compost and antagonistic fungi (Trichoderma harzianum, Papulaspora sp. and Paecylomyces sp.) to enhance tomato resistance to early blight disease (Alternaria solani) and also to obtain the effective and efficient application methods of compost plus for enhancing the plant resistance. The results showed that compos, compost plus and T. harzianum enhanced the tomato resistance so that suppressed early blight disease by 39.2%-40.1%. The application methods were not significantly influenced the effectiveness of compost plus in enhancing the plant resistance. The application of compost plus as seed dressing, in the planting site or spraying water extract onto leaves solely or in combination suppress early blight disease by 31.96%-42.08%. The separation between the treatments and pathogen inoculation indicated the increase or induction of tomato resistance to the disease.Keywords: Antagonistic microbes, Trichoderma harzianum, Application method, Induced resistanceABSTRAKKompos selain dapat berfungsi sebagai pupuk organik juga dapat dimanfaatkan untuk pengendalian penyakit. Guna meningkatkan keefektifannya, kompos ditambah dengan jamur antagonis dan disebut ‘kompos plus’. Penelitian ini bertujuan untuk menguji kemampuan kompos plus dan komponen penyusunnya yaitu kompos dan jamur antagonis (Trichoderma harzianum, Papulaspora sp. dan Paecylomyces sp.) untuk meningkatkan ketahanan tanaman tomat terhadap penyakit bercak coklat dan mendapatkan cara aplikasi kompos plus yang efektif dan efisien untuk meningkatkan ketahanan tanaman. Hasil percobaan menunjukkan bahwa kompos plus, kompos, dan T. harzianum dapat meningkatkan ketahanan tanaman tomat sehingga menekan penyakit bercak coklat sebesar 39,2%-40,1%. Cara aplikasi kompos plus tidak berpengaruh secara nyata terhadap kemampuannya dalam meningkatkan ketahanan tanaman. Aplikasi kompos plus dengan cara perendaman benih dalam air rendaman kompos plus, aplikasi pada lubang tanam atau penyemprotan dengan air rendaman kompos baik secara tunggal maupun dengan cara gabungan dapat menekan penyakit bercak coklat sebesar 31,96%-42,08%. Pemisahan tempat antara perlakuan dengan inokulasi patogen mengindikasikan adanya peningkatan atau induksi ketahanan tanaman terhadap penyakit.Kata Kunci: Mikroba antagonis, Trichoderma harzianum, Cara aplikasi, Induksi ketahanan

scholarly journals Evaluation of various plant extracts against the early blight disease of tomato plants under greenhouse and field conditions

2012 ◽  
Vol 48 (No. 2) ◽  
pp. 74-79 ◽  
Author(s):  
S.M.A. Nashwa ◽  
K.A.M. Abo-Elyousr
Keyword(s):  
Disease Severity ◽  
Plant Extracts ◽  
Mycelial Growth ◽  
Early Blight ◽  
Field Conditions ◽  
Nerium Oleander ◽  
Tomato Plants ◽  
Infected Control ◽  
Blight Disease

The antimicrobial activity of six plant extracts from Ocimum basilicum (Sweat Basil), Azadirachta indica (Neem), Eucalyptus chamadulonsis (Eucalyptus), Datura stramonium (Jimsonweed), Nerium oleander (Oleander), and Allium sativum (Garlic) was tested for controlling Alternaria solani in vitro and in vivo. In in vitro study the leaf extracts of D. stramonium, A. indica, and A. sativum at 5% concentration caused the highest reduction of mycelial growth of A. solani (44.4, 43.3 and 42.2%, respectively), while O. basilicum at 1% and 5% concentration and N. oleander at 5% concentration caused the lowest inhibition of mycelial growth of the pathogen. In greenhouse experiments the highest reduction of disease severity was achieved by the extracts of A. sativum at 5% concentration and D. stramonium at 1% and 5% concentration. The greatest reduction of disease severity was achieved by A. sativum at 5% concentration and the smallest reduction was obtained when tomato plants were treated with O. basilicum at 1% and 5% concentration (46.1 and 45.2 %, respectively). D. stramonium and A. sativum at 5% concentration increased the fruit yield by 76.2% and 66.7% compared to the infected control. All treatments with plant extracts significantly reduced the early blight disease as well as increased the yield of tomato compared to the infected control under field conditions.

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