scholarly journals Ribotyping to compare Fusobacterium necrophorum isolates from bovine liver abscesses, ruminal walls, and ruminal contents.

1997 ◽  
Vol 63 (12) ◽  
pp. 4671-4678 ◽  
Author(s):  
S Narayanan ◽  
T G Nagaraja ◽  
O Okwumabua ◽  
J Staats ◽  
M M Chengappa ◽  
...  
Keyword(s):  
Bovine Liver ◽  
Fusobacterium Necrophorum ◽  
Liver Abscesses

scholarly journals Extraction and Titration of Leukotoxins from Fusobacterium necrophorum Isolates Recovered from Bovine Liver Abscesses in Sulaimaniyah Region

2012 ◽  
Vol 36 (0E) ◽  
pp. 104-109
Author(s):  
Salameh Sh. Barhoom ◽  
Suha Ali Hussein
Keyword(s):  
Bovine Liver ◽  
Fusobacterium Necrophorum ◽  
Reduction Test ◽  
Liver Abscesses ◽  
Dye Reduction ◽  
Culture Supernatants

This study was conducted to extract and titrate the leukotoxin of Fusobacteriumnecrophorum isolates recovered from 75abscesses found in 24 livers of slaughtered cattle inSulaimaniyah region. The culture supernatants of these isolates were subjected to thetetrazolium dye reduction test which revealed that the leukotoxin titer values of 34 F.necrophorum subsp. necrophorum isolates ranged from 128 to 1024 (with a leukotoxin titermean of 516±46), whereas the leukotoxin titer values of the 11 F. necrophorum subsp.funduliforme isolates ranged from 0 to 128 (with a leukotoxin titer mean of 73±12).

scholarly journals Extraction and Titration of Leukotoxins from Fusobacterium necrophorum Isolates Recovered from Bovine Liver Abscesses in Sulaimaniyah Region

2012 ◽  
Vol 36 (0E) ◽  
pp. 110-114
Author(s):  
Suha Ali Hussein
Keyword(s):  
Bovine Liver ◽  
Fusobacterium Necrophorum ◽  
Reduction Test ◽  
Liver Abscesses ◽  
Dye Reduction ◽  
Culture Supernatants

This study was conducted to extract and titrate the leukotoxin of Fusobacterium necrophorum isolates recovered from 75abscesses found in 24 livers of slaughtered cattle in Sulaimaniyah region. The culture supernatants of these isolates were subjected to the tetrazolium dye reduction test which revealed that the leukotoxin titer values of 34 F. necrophorum subsp. necrophorum isolates ranged from 128 to 1024 (with a leukotoxin titer mean of 516±46), whereas the leukotoxin titer values of the 11 F. necrophorum subsp. funduliforme isolates ranged from 0 to 128 (with a leukotoxin titer mean of 73±12).

scholarly journals Isolation of Sphaerophorus necrophorus from Bovine Liver Abscesses

1967 ◽  
Vol 15 (6) ◽  
pp. 1492-1493
Author(s):  
H. E. Calkins ◽  
L. H. Scrivner
Keyword(s):  
Bovine Liver ◽  
Liver Abscesses

Hepatic lesions and bacterial changes in mice during infection of Fusobacterium necrophorum

1977 ◽  
Vol 23 (10) ◽  
pp. 1465-1477 ◽  
Author(s):  
M. M. Garcia ◽  
K. M. Charlton ◽  
K. A. McKay
Keyword(s):  
Mononuclear Cells ◽  
Plasma Cells ◽  
Inflammatory Cells ◽  
Fusobacterium Necrophorum ◽  
Post Inoculation ◽  
Liver Abscesses ◽  
Infected Liver ◽  
Ultrathin Sections ◽  
Hepatic Lesions ◽  
Intraperitoneal Inoculation

Liver abscesses were induced in male albino mice within 1 week after intraperitoneal inoculation of viable Fusobacterium necrophorum LA 19 culture. Fusobacteremia was transitory and reached a peak 2 h after inoculation then sharply declined until its disappearance 24 h post inoculation. By contrast, the number of fusobacteria in the liver increased rapidly during the first 4 h post inoculation and continued to do so less rapidly until the last sampling time (48 h post inoculation). There were small or large areas of necrosis, usually surrounded by inflammatory cells, small focal accumulations of lymphocytes, plasma cells, and macrophages in areas of parenchyma with no degenerations, generalized proliferation of Kupffer cells, and a few accumulations of fibrin and leukocytes on the surface. Ultrathin sections of infected liver tissues revealed both intact and partially degraded F. necrophorum cells enclosed in phagocytic and digestive vacuoles of mononuclear cells. The results indicate that macrophages play a key role in the pathogenesis of liver abscesses.

scholarly journals Characterization of bacterial DNA identified in abscessed and non-abscessed bovine hepatic tissue at the time of harvest

2021 ◽  
Author(s):  
Miranda K Stotz ◽  
Darren D Henry ◽  
Whitney L Crossland
Keyword(s):  
Liver Tissue ◽  
Bovine Liver ◽  
Fusobacterium Necrophorum ◽  
Laboratory Culture ◽  
Hepatic Tissue ◽  
Rrna Gene ◽  
Pathogenic Potential ◽  
Bacterial Dna ◽  
Cultural Methods

Abstract Bacteriological characterization of bovine liver abscesses has been accomplished by cultural methods but DNA methods are still needed, as many bacteria are not conducive to laboratory culture. In addition to this gap in research, there have been no studies which identify the bacterial presence within healthy, non-abscessed liver tissue. The objective of this study was to compare the bacteriome of both abscessed and non-abscessed bovine livers in an observational case-control study design. Fifty-six livers, obtained from Holstein steers, were scored according to a modified Elanco liver abscess score description where A- was partitioned into active abscesses or scarred where only scars were present. Parenchyma tissue was collected from non-abscessed livers (n=22), and scarred livers (n=7), and purulent material was collected from abscessed livers (n=24), and DNA was extracted for 16s rRNA gene sequence-based bacterial analysis. Across liver samples, 21 total phyla were identified with a mean of 14. Predominant phyla, accounting for > 98% of reads, were Fusobacteria (51.7%), Bacteroidetes (26.9%), Proteobacteria (8.03%), Firmicutes (5.39%), Cyanobacteria (3.85 %) and Actinobacteria (2.21%). Proteobacteria, Cyanobacteria and Firmicutes were greater in non-abscessed and scarred livers, whereas Fusobacteria and Bacteroidetes prevailed in abscessed livers. Non-abscessed livers shared 3,059 operational taxonomic units (OTU) with abscessed livers (total OTU of all livers= 4,167), but non-abscessed livers had greater richness and evenness whereas abscessed livers had greater dominance (P ≤ 0.0014). Liver score affected the relative abundance of OTU (R = 0.463; P = 0.001) but abscessed livers shared ≥ 40% similarity and were not different from each other (P ≥ 0.370). Of the predominant OTU (top 10 as a % of reads), three OTU (Fusobacteria necrophorum, Bacteroides spp., and Trueperella pyogenes) were shared across both abscessed and non-abscessed livers. Fusobacterium necrophorum was the dominant OTU regardless of liver score, and the single most abundant OTU, even among non-abscessed livers. We describe bacterial DNA detected in non-abscessed bovine liver tissue for the first time, which indicates possible presence of viable bacteria with pathogenic potential in apparently healthy liver tissue.

scholarly journals A rare case of Fusobacterium necrophorum liver abscesses

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Faris Hannoodi ◽  
Hussam Sabbagh ◽  
Zain Kulairi ◽  
Sarwan Kumar
Keyword(s):  
Rare Condition ◽  
Fusobacterium Necrophorum ◽  
Peritonsillar Abscess ◽  
Care Physician ◽  
Source Of Infection ◽  
Liver Abscesses ◽  
Uncommon Disease ◽  
Night Sweats ◽  
Right Lobe ◽  
The Right

Liver abscesses are an uncommon disease that can present with vague symptoms. <em>Fusobacterium necrophorum</em> causing liver abscesses is a rare condition and only a few cases have been reported. An 88-year-old female presented to her primary care physician with one week of fevers, night sweats, chills, fatigue and vague right upper quadrant abdominal pain. She denied nausea, vomiting, constipation, diarrhea and unintentional weight loss. A computed tomography scan of the abdomen showed two liver abscesses in the right lobe as well as extensive diverticulosis. Percutaneous drainage was performed and draining catheters were placed in the abscesses. Culture of the abscess fluid grew <em>Fusobacterium necrophorum</em>. She was treated with ceftriaxone and metronidazole as per sensitivities. Rare cases of <em>F. necrophorum</em> hepatic abscesses have been published. The source of infection described in reported cases included hematogenous spread from dental caries/peritonsillar abscess and those involving the gastrointestinal tract resulting from inflammation of the bowel wall or from inflamed diverticuli via the portal circulation. In one study, thirteen cases of liver abscess due to <em>F. necrophorum</em> were studied, and two of these cases had diverticular disease without inflammation.

Isolation ofSphaerophorus necrophorus from bovine liver abscesses in the Sudan

1974 ◽  
Vol 6 (4) ◽  
pp. 253-254 ◽  
Author(s):  
H. E. Hussein ◽  
M. T. A. Shigidi
Keyword(s):  
Bovine Liver ◽  
Liver Abscesses
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