Ribotyping to differentiate Fusobacterium necrophorum subsp. necrophorum and F. necrophorum subsp. funduliforme isolated from bovine ruminal contents and liver abscesses.

This study was conducted to extract and titrate the leukotoxin of Fusobacteriumnecrophorum isolates recovered from 75abscesses found in 24 livers of slaughtered cattle inSulaimaniyah region. The culture supernatants of these isolates were subjected to thetetrazolium dye reduction test which revealed that the leukotoxin titer values of 34 F.necrophorum subsp. necrophorum isolates ranged from 128 to 1024 (with a leukotoxin titermean of 516±46), whereas the leukotoxin titer values of the 11 F. necrophorum subsp.funduliforme isolates ranged from 0 to 128 (with a leukotoxin titer mean of 73±12).

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Extraction and Titration of Leukotoxins from Fusobacterium necrophorum Isolates Recovered from Bovine Liver Abscesses in Sulaimaniyah Region

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v36i0e.403 ◽

2012 ◽

Vol 36 (0E) ◽

pp. 110-114

Author(s):

Suha Ali Hussein

Keyword(s):

Bovine Liver ◽

Fusobacterium Necrophorum ◽

Reduction Test ◽

Liver Abscesses ◽

Dye Reduction ◽

Culture Supernatants

This study was conducted to extract and titrate the leukotoxin of Fusobacterium necrophorum isolates recovered from 75abscesses found in 24 livers of slaughtered cattle in Sulaimaniyah region. The culture supernatants of these isolates were subjected to the tetrazolium dye reduction test which revealed that the leukotoxin titer values of 34 F. necrophorum subsp. necrophorum isolates ranged from 128 to 1024 (with a leukotoxin titer mean of 516±46), whereas the leukotoxin titer values of the 11 F. necrophorum subsp. funduliforme isolates ranged from 0 to 128 (with a leukotoxin titer mean of 73±12).

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Hepatic lesions and bacterial changes in mice during infection of Fusobacterium necrophorum

Canadian Journal of Microbiology ◽

10.1139/m77-215 ◽

1977 ◽

Vol 23 (10) ◽

pp. 1465-1477 ◽

Cited By ~ 3

Author(s):

M. M. Garcia ◽

K. M. Charlton ◽

K. A. McKay

Keyword(s):

Mononuclear Cells ◽

Plasma Cells ◽

Inflammatory Cells ◽

Fusobacterium Necrophorum ◽

Post Inoculation ◽

Liver Abscesses ◽

Infected Liver ◽

Ultrathin Sections ◽

Hepatic Lesions ◽

Intraperitoneal Inoculation

Liver abscesses were induced in male albino mice within 1 week after intraperitoneal inoculation of viable Fusobacterium necrophorum LA 19 culture. Fusobacteremia was transitory and reached a peak 2 h after inoculation then sharply declined until its disappearance 24 h post inoculation. By contrast, the number of fusobacteria in the liver increased rapidly during the first 4 h post inoculation and continued to do so less rapidly until the last sampling time (48 h post inoculation). There were small or large areas of necrosis, usually surrounded by inflammatory cells, small focal accumulations of lymphocytes, plasma cells, and macrophages in areas of parenchyma with no degenerations, generalized proliferation of Kupffer cells, and a few accumulations of fibrin and leukocytes on the surface. Ultrathin sections of infected liver tissues revealed both intact and partially degraded F. necrophorum cells enclosed in phagocytic and digestive vacuoles of mononuclear cells. The results indicate that macrophages play a key role in the pathogenesis of liver abscesses.

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A rare case of Fusobacterium necrophorum liver abscesses

Clinics and Practice ◽

10.4081/cp.2017.928 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 2

Author(s):

Faris Hannoodi ◽

Hussam Sabbagh ◽

Zain Kulairi ◽

Sarwan Kumar

Keyword(s):

Rare Condition ◽

Fusobacterium Necrophorum ◽

Peritonsillar Abscess ◽

Care Physician ◽

Source Of Infection ◽

Liver Abscesses ◽

Uncommon Disease ◽

Night Sweats ◽

Right Lobe ◽

The Right

Liver abscesses are an uncommon disease that can present with vague symptoms. Fusobacterium necrophorum causing liver abscesses is a rare condition and only a few cases have been reported. An 88-year-old female presented to her primary care physician with one week of fevers, night sweats, chills, fatigue and vague right upper quadrant abdominal pain. She denied nausea, vomiting, constipation, diarrhea and unintentional weight loss. A computed tomography scan of the abdomen showed two liver abscesses in the right lobe as well as extensive diverticulosis. Percutaneous drainage was performed and draining catheters were placed in the abscesses. Culture of the abscess fluid grew Fusobacterium necrophorum. She was treated with ceftriaxone and metronidazole as per sensitivities. Rare cases of F. necrophorum hepatic abscesses have been published. The source of infection described in reported cases included hematogenous spread from dental caries/peritonsillar abscess and those involving the gastrointestinal tract resulting from inflammation of the bowel wall or from inflamed diverticuli via the portal circulation. In one study, thirteen cases of liver abscess due to F. necrophorum were studied, and two of these cases had diverticular disease without inflammation.

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Fusobacterium necrophorum Endocarditis with Liver Abscesses: A Case Report and Review of the Literature

Internal Medicine ◽

10.2169/internalmedicine.6348-20 ◽

2021 ◽

Author(s):

Kaho Sato ◽

Taku Matsubara ◽

Shunsuke Imai ◽

Katsuharu Hatada ◽

Wataru Mitsuma ◽

...

Keyword(s):

Case Report ◽

Fusobacterium Necrophorum ◽

Review Of The Literature ◽

Liver Abscesses

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Antibiotic susceptibility of Fusobacterium necrophorum isolated from liver abscesses

Kansas Agricultural Experiment Station Research Reports ◽

10.4148/2378-5977.1895 ◽

1998 ◽

pp. 50-52

Author(s):

K.F. Lechtenberg ◽

Tiruvoor G. Nagaraja ◽

M. M. Chengappa

Keyword(s):

Antibiotic Susceptibility ◽

Fusobacterium Necrophorum ◽

Liver Abscesses

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Fusobacterium necrophorum leukotoxoid vaccine for prevention of liver abscesses

Kansas Agricultural Experiment Station Research Reports ◽

10.4148/2378-5977.1889 ◽

1998 ◽

pp. 40-43

Author(s):

S. Saginala ◽

K.F. Lechtenberg ◽

Kenneth E. Kemp ◽

P.M. Hine ◽

Tiruvoor G. Nagaraja ◽

...

Keyword(s):

Fusobacterium Necrophorum ◽

Liver Abscesses

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Ribotyping to compare Fusobacterium necrophorum isolates from bovine liver abscesses, ruminal walls, and ruminal contents.

Applied and Environmental Microbiology ◽

10.1128/aem.63.12.4671-4678.1997 ◽

1997 ◽

Vol 63 (12) ◽

pp. 4671-4678 ◽

Cited By ~ 20

Author(s):

S Narayanan ◽

T G Nagaraja ◽

O Okwumabua ◽

J Staats ◽

M M Chengappa ◽

...

Keyword(s):

Bovine Liver ◽

Fusobacterium Necrophorum ◽

Liver Abscesses

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PSIV-6 Effect of a Direct Fed Microbial Feed Additive on Finishing Beef Cattle Performance and Liver Abscess Rate

Journal of Animal Science ◽

10.1093/jas/skab054.348 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 212-212

Author(s):

Kelton Adair ◽

Samodha Fernando ◽

Galen E Erickson ◽

Alison Bartenslager ◽

Zachary E Carlson

Keyword(s):

Beef Cattle ◽

Liver Abscess ◽

Average Daily Gain ◽

Fusobacterium Necrophorum ◽

Feed Additive ◽

Bacterial Cells ◽

Liver Abscesses ◽

Significant Difference ◽

Gate System ◽

Finishing Beef Cattle

Abstract A feedlot study was conducted comparing the effects of a direct-fed microbial feed additive (DFM) to no feed additive (CON) on performance and liver abscess rates in finishing beef cattle. The study utilized 60 crossbred steers (initial BW 274 kg ± 2.23) individually fed using a Calan gate system. Steers were housed in separate pens by treatment to avoid DFM cross-contamination, with pen (barn of 30 steers) assigned randomly to each treatment. Cattle were fed a diet consisting of 15% corn silage, 36.5% high moisture corn, 24.5% dry rolled corn, 20% modified distillers grains, and 4% supplement for 189 days. The DFM counts were estimated using cell cytometry and was top dressed at a concentration of approximately 81 billion bacterial cells/head/day. The DFM additive used in this study was developed to reduce the abundance of Fusobacterium necrophorum and Streptococcus bovis in the rumen. No effect of treatment on hot carcass weight (HCW), average daily gain (ADG), dry matter intake (DMI), feed efficiency (G:F), or carcass traits (Table 1) were observed. No significant difference in the occurrence of liver abscesses between treatment groups were observed with 4 steers having abscessed livers in the CON group and 3 steers in the DFM group. Additionally, there were no differences in the severity of liver abscesses; all observed liver abscesses received the score of A. The DFM utilized in this study did not significantly affect performance, liver abscess rate, or the severity of liver abscesses in finishing beef cattle.

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