scholarly journals SELECTION OF OPTIMAL METHOD OF THE PATHOGEN DNA ISOLATION OF GRAPE FLAYESCENE DOREE AND BOIS NOIR TO IDENTIFY PATHOGEN BY REAL TIME-PCR

2021 ◽  
Vol 1 (67) ◽  
pp. 283-293
Author(s):  
Victoria Konstantinovna Kotlyar ◽  
◽  
Marina Victorovna Makarkina ◽  
Ilya Vladimirovich Stepanov ◽  
Elena Tarasovna Ilnitskaya ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Isolation ◽  
Optimal Method ◽  
Bois Noir ◽  
Pathogen Dna ◽  
Selection Of

Real-time PCR-assisted selection of wheat plants transformed with HMW glutenin subunit genes

2005 ◽  
Vol 41 (1) ◽  
pp. 133-136 ◽  
Author(s):  
Valeria Terzi ◽  
Gabriela Pastori ◽  
Peter R. Shewry ◽  
Natale Di Fonzo ◽  
A. Michele Stanca ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Glutenin Subunit ◽  
Hmw Glutenin Subunit ◽  
Hmw Glutenin ◽  
Selection Of

A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis

2009 ◽  
Vol 129 (1-2) ◽  
pp. 115-118 ◽  
Author(s):  
Yvette M. Schlotter ◽  
Eveline Z. Veenhof ◽  
Bas Brinkhof ◽  
Victor P.M.G. Rutten ◽  
Bart Spee ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Skin Biopsies ◽  
Healthy Dogs ◽  
Selection Of

scholarly journals Comparison of different conditions for DNA extraction in sputum - a pilot study

2019 ◽  
Vol 14 ◽  
Author(s):  
Martina Oriano ◽  
Leonardo Terranova ◽  
Antonio Teri ◽  
Samantha Sottotetti ◽  
Luca Ruggiero ◽  
...  
Keyword(s):  
Real Time ◽  
16S Rdna ◽  
Dna Extraction ◽  
Real Time Pcr ◽  
Alpha Diversity ◽  
Diversity Indices ◽  
Extraction Yield ◽  
Chronic Respiratory Diseases ◽  
Dna Yield ◽  
Selection Of

Background: The analysis of microbiome in respiratory samples is a topic of great interest in chronic respiratory diseases. The method used to prepare sputum samples for microbiome analysis is very heterogeneous. The selection of the most suitable methodology for DNA extraction is fundamental to have the most representative data. The objective of this study was to compare different conditions for DNA extraction from sputum in adult patients with bronchiectasis. Methods: Five sputum samples from bronchiectasis patients were collected at the Policlinico Hospital in Milan, Italy. Eighteen conditions for DNA extraction were compared, including two enzyme-based (Roche and Zymo) and one beads-based (Mobio) technique. These techniques were tested with/without Dithiothreitol (DTT) and with/without lysostaphin (0.18 and 0.36 mg/mL) step. DNA was quantified, tested using Real-time PCR for 16S rDNA and S. aureus and, then, microbiome was evaluated. Results: Although 16S rDNA was similarly detected across all the different techniques, Roche kit gave the highest DNA yield. The lowest Ct values for Real-time PCR for S. aureus was identified when lysostaphin was added. Considering genera from microbiome, alpha diversity indices did not show any significant differences between techniques, while relative abundances were more similar in presence of DTT. Conclusions: None of the conditions emerged to be superior to the others even if enzyme-based kits seem to be needed in order to have a higher extraction yield.

Selection of suitable reference genes for quantitive real-time PCR normalization in Miscanthus lutarioriparia

2019 ◽  
Vol 46 (4) ◽  
pp. 4545-4553 ◽  
Author(s):  
Teng Cheng ◽  
Fenglin Zhu ◽  
Jiajing Sheng ◽  
Lingling Zhao ◽  
Fasong Zhou ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Suitable Reference ◽  
Selection Of

scholarly journals Selection of reference genes for quantitative real-time PCR in Casuarina equisetifolia under salt stress

2017 ◽  
Vol 61 (3) ◽  
pp. 463-472 ◽  
Author(s):  
C. Fan ◽  
Z. Qiu ◽  
B. Zeng ◽  
Y. Liu ◽  
X. Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Casuarina Equisetifolia ◽  
Quantitative Real Time Pcr ◽  
Selection Of

scholarly journals A tissue-based approach to selection of reference genes for quantitative real-time PCR in a sheep osteoporosis model

BMC Genomics ◽  
2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Felix Schulze ◽  
Deeksha Malhan ◽  
Thaqif El Khassawna ◽  
Christian Heiss ◽  
Anja Seckinger ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Selection Of

Selection of suitable reference genes for reverse transcription quantitative real-time PCR studies on different experimental systems from carrot (Daucus carota L.)

2015 ◽  
Vol 186 ◽  
pp. 115-123 ◽  
Author(s):  
Maria Doroteia Campos ◽  
António Miguel Frederico ◽  
Thomas Nothnagel ◽  
Birgit Arnholdt-Schmitt ◽  
Hélia Cardoso
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Daucus Carota ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Daucus Carota L ◽  
Experimental Systems ◽  
Suitable Reference ◽  
Selection Of

scholarly journals Selection of Suitable Reference Genes for Quantitative Real-time PCR in Sapium sebiferum

2017 ◽  
Vol 8 ◽  
Author(s):  
Xue Chen ◽  
Yingji Mao ◽  
Shengwei Huang ◽  
Jun Ni ◽  
Weili Lu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Sapium Sebiferum ◽  
Quantitative Real Time Pcr ◽  
Suitable Reference ◽  
Selection Of
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