scholarly journals Effects of Buprenorphine, Chlorhexidine, and Low-level Laser Therapy on Wound Healing in Mice

2021 ◽  
Author(s):  
Donna R Webb ◽  
Sheba R Churchill ◽  
Georgette D Hill ◽  
Christopher A McGee ◽  
Min Shi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Laser Therapy ◽  
Punch Biopsy ◽  
Low Level Laser Therapy ◽  
Treatment Modalities ◽  
Wound Area ◽  
Treatment Groups ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

Systemic buprenorphine and topical antiseptics such as chlorhexidine are frequently used in research animals to aid inpain control and to reduce infection, respectively. These therapeutics are controversial, especially when used in wound healing studies, due to conflicting data suggesting that they delay wound healing. Low-level laser therapy (LLLT) has been used to aid in wound healing without exerting the systemic effects of therapies such as buprenorphine. We conducted 2 studies to investigate the effects of these common treatment modalities on the rate of wound healing in mice. The first study used models of punch biopsy and dermal abrasion to assess whether buprenorphine HCl or 0.12% chlorhexidine delayed woundhealing. The second study investigated the effects of sustained-released buprenorphine, 0.05% chlorhexidine, and LLLT on excisional wound healing. The rate of wound healing was assessed by obtaining photographs on days 0, 2, 4, 7, and 9 for the punch biopsy model in study 1, days 0, 1, 2, 4, 6, 8, 11, and 13 for the dermal abrasion model in study 1, and days 0, 3, 6, and 10 for the mice in study 2. Image J software was used to analyze the photographed wounds to determine the wound area.When comparing the wound area on the above days to the original wound area, no significant differences in healing wereobserved for any of the treatment groups at any time period for either study. Given the results of these studies, we believe that systemic buprenorphine, topical chlorhexidine, and LLLT can be used without impairing or delaying wound healing in mice.

scholarly journals Healing of Skin Wounds in Rats: Influence of Low-Level Laser Therapy, Alone or Combined with Natural Products

2020 ◽  
Vol 48 ◽  
Author(s):  
Diana Milanez Ávila Dias Maciel ◽  
Sheila Canevese Rahal ◽  
Carlos Eduardo Fonseca Alves ◽  
Ivan Felismino Charas dos Santos ◽  
Alexandre Botelho De Abreu Sampaio ◽  
...  
Keyword(s):  
Wound Healing ◽  
Natural Products ◽  
Laser Therapy ◽  
Inflammatory Infiltrate ◽  
Low Level Laser Therapy ◽  
Skin Wounds ◽  
Wound Area ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

Background: A great variety of natural products have been evaluated for the skin wound healing due to anti-inflammatory, antioxidant and antibacterial activities, and procollagen synthesis, of which may be mentioned the honey and propolis. In turn, low-level laser therapy (LLLT) is considered an important tool in the treatment of skin wounds, because of ability to raise the ATP production, to stimulate the microcirculation, and formation of new blood vessels. Therefore,this study aimed to assess the influence of LLLT, alone or combined with natural products, in the healing of excisional skin wounds in rats. Materials, Methods & Results:  One hundred twenty-six male Wistar rats, aged 14 weeks, were randomly divided into seven groups (n = 18 per group) according to wound treatments: G1 (negative control): 0.9% saline solution; G2 (positive control): allantoin + zinc oxide ointment; G3: LLLT; G4: mixture of honey + 5% propolis hydroalcoholic solution; G5: LLLT + honey + hydroalcoholic solution of 5% propolis; G6: LLLT + honey in natura; G7: LLLT + 5% propolis hydroalcoholic solution. Six rats from each group were euthanized on the 7th, 14th, and 21th postoperative days. Macroscopic and histological evaluations of wound healing were performed. A decrease in wound area occurred in all groups, but in general G4 had the higher reduction and G1 and G3 had the lower reductions. These findings corresponded to the percentage of wound contraction, since the largest contraction was G4 and the lowest contractions were G1 and G3. Histological analysis showed no significant difference among groups on the 7th postoperative day. On postoperative day 14, a significant decrease in hemorrhage occurred between G1xG4, G1xG5 and G1xG7, and a significant decrease in congestion between G1xG7 and G2xG5. In addition, intensity of the inflammatory infiltrate showed a significant reduction between G1xG7, G2xG7 and G3xG7. On postoperative day 21, a significant changed from mixed to mononuclear inflammatory infiltrate was observed between G1xG3, G1xG4, G1xG6, G1xG7, G2xG3, and G2xG6. Regarding organization of fibroblasts, there were differences between G1xG6 and G1xG7. Discussion: Spite the red laser has been associated to in increase fibroblast proliferation, G3 showed inadequate decrease in wound area and percentage of contraction. Histological examination showed no differences among groups in the inflammatory phase. However, in the proliferative and remodeling phases around days 14 and 21 were found some differences among groups, which may be related to the actions of the natural products, or laser. Studies have shown that honey acts as a repair and anti-inflammatory agent in skin woundthat may be associated to lower hemorrhage in G4 and G4 compared to G1 on day 14. Propolis is related to reduction of free radicals and increasing the amount of collagen, which may have contributed for quality improvement of the inflammatory infiltrate in G4 and G7 compared to G1, and fibroblast organization in G7 at postoperative day 21. In conclusion, macroscopically the mixture of honey + 5% propolis hydroalcoholic solution was the most effective in reducing wound area and increasing wound contraction. However, based on the parameters evaluated histologically, may be highlighted the evolution of the treatments with LLLT + honey, as well as LLLT + 5% propolis hydroalcoholic solution. Keywords: honey, propolis, biomodulation, wound, healing.

Wound-healing effects of low-level laser therapy in diabetic rats involve the modulation of MMP-2 and MMP-9 and the redistribution of collagen types I and III

2013 ◽  
Vol 15 (4) ◽  
pp. 210-216 ◽  
Author(s):  
Andreia Aparecida Da Silva ◽  
Ernesto Cesar Pinto Leal-Junior ◽  
Ana Carolina Araruna Alves ◽  
Caroline Sobral Rambo ◽  
Solange Almeida Dos Santos ◽  
...  
Keyword(s):  
Wound Healing ◽  
Laser Therapy ◽  
Diabetic Rats ◽  
Low Level Laser Therapy ◽  
Collagen Types ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

scholarly journals Effect of low-level laser therapy on wound healing after depigmentation procedure: A clinical study

2016 ◽  
Vol 0 (0) ◽  
pp. 0 ◽  
Author(s):  
Kirti Chawla ◽  
ArundeepKaur Lamba ◽  
Shruti Tandon ◽  
Farrukh Faraz ◽  
Varun Gaba
Keyword(s):  
Wound Healing ◽  
Clinical Study ◽  
Laser Therapy ◽  
Low Level Laser Therapy ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

Low-Level Laser-Assisted Liposuction: A Multicenter Side-by-Side Comparison Study

2003 ◽  
Vol 20 (2) ◽  
pp. 97-102 ◽  
Author(s):  
Edward B. Lack ◽  
Kimberly J. Butterwick ◽  
Neil Sadick
Keyword(s):  
Wound Healing ◽  
Laser Therapy ◽  
Local Anesthesia ◽  
The United States ◽  
Low Level Laser Therapy ◽  
Tumescent Technique ◽  
Postoperative Course ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

Introduction: Liposuction has undergone many revisions since 1980, the most notable being the introduction of the tumescent technique of local anesthesia by Jeffery Klein, MD. Since then, in an effort to improve the aesthetics of the technique and the ease and safety of the procedure, other techniques have been introduced. These include superficial liposuction, UAL, external ultrasonic liposuction, mechanically assisted liposuction, and, most recently, laser-assisted liposuction. Materials and Methods: Four centers in the United States, operating under an 1RB protocol, performed liposuction with the tumescent technique of local anesthesia on specified cosmetic units of the body. One side was exposed to 635-nm laser therapy after tumescence was achieved, whereas the contralateral side was not. Observations were recorded during surgery and at 2 weeks postoperatively. Results: Observations of intraoperative technique did not show an advantage to laser-assisted liposuction. Observations of the postoperative course were equivocal, though there was evidence of reduction of edema in certain patients. Discussion: The addition of laser therapy to liposuction technique is based on years of experience demonstrating improved wound healing using a technique of low-level laser therapy. Although the recorded observations did not support an advantage to the addition of laser therapy, there was a paradoxical difference of opinion among patients and surgeons who participated in the study. These individuals believed that, compared with patients not treated with low-level laser therapy, there was at least a reduction in degree and duration of postoperative pain and induration. As low-level laser therapy may have systemic effects on wound healing, comparing treated and untreated contralateral sides may not be valid because both sides experienced improvements in postoperative course. Laser-assisted liposuction has a potential advantage, not supported by this study, in the intraoperative and postoperative course of liposuction surgery. New studies using double-blind techniques will need to be conducted.

scholarly journals Low-Level Laser Therapy with 810 nm Wavelength Improves Skin Wound Healing in Rats with Streptozotocin-Induced Diabetes

2014 ◽  
Vol 32 (4) ◽  
pp. 198-204 ◽  
Author(s):  
Ludmila Dancáková ◽  
Tomáš Vasilenko ◽  
Ivan Kováč ◽  
Katarína Jakubčová ◽  
Martin Hollý ◽  
...  
Keyword(s):  
Wound Healing ◽  
Laser Therapy ◽  
Skin Wound ◽  
Low Level Laser Therapy ◽  
Skin Wound Healing ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Streptozotocin Induced Diabetes

scholarly journals Effectiveness of Low-Level Laser Therapy with a 915 Nm Wavelength Diode Laser on the Healing of Intraoral Mucosal Wound: An Animal Study and a Double-Blind Randomized Clinical Trial

Medicina ◽  
2019 ◽  
Vol 55 (8) ◽  
pp. 405 ◽  
Author(s):  
Choung ◽  
Lee ◽  
Ham ◽  
Lee ◽  
Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Clinical Study ◽  
Laser Therapy ◽  
Third Molar ◽  
Low Level Laser Therapy ◽  
Low Level ◽  
Low Level Laser ◽  
Third Molar Extraction ◽  
Level Laser ◽  
Mucosal Wound

Background and objectives: Diode laser has been the most popular low-level laser therapy (LLLT) technique in dentistry due to its good tissue penetration, lower financial costs, small size for portable application, and convenience to use. A series of recent studies with 940 nm or 980 nm lasers demonstrated that LLLT showed positive effects after third molar extraction or periodontal flap surgery. However, the effects of LLLT on intraoral mucosal wound healing after surgical incision have not yet been determined in human clinical study. Materials and Methods: The present study was performed to determine the efficacy and safety of 915 nm wavelength low-level laser therapy (LLLT) in mucosal wound healing. A total of 108 Sprague–Dawley rats were used. They were divided into three groups: Abrasive wound group, immediate LLLT once group, and daily LLLT group. As a clinical study, a total of 16 patients with split-mouth design subjected to bilateral mandibular third molar extraction were allocated into the LLLT group and placebo group. The process of LLLT was performed on postoperative days 0, 1, and 7, and parameters related to wound healing were analyzed on days 1, 7, and 14. Results: Repeated laser irradiation promoted mucosal wound healing of the rats. In the clinical study, although there were no significant statistical differences between the LLLT and placebo groups in all inflammatory parameters, the early stage mucosal healing tendency of wound dehiscence was higher in the LLLT group than in the placebo group clinically on postoperative day 1. Conclusions: The present results showed that 915 nm LLLT could be applied safely as an auxiliary therapy for mucosal wound healing.

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