scholarly journals A strong inhibitor of chymotrypsin/elastase is highly antimetabolic to Helicoverpa armigera larvae

2002 ◽  
Vol 55 ◽  
pp. 421-428 ◽  
Author(s):  
L.N. Gatehouse ◽  
J.T. Christeller ◽  
H.S. Gatehouse ◽  
X.Y. Zou
Keyword(s):  
Protease Inhibitors ◽  
Helicoverpa Armigera ◽  
Active Site ◽  
Artificial Diet ◽  
Rna Expression ◽  
Strong Inhibitor ◽  
Experimental Animals ◽  
Gene Group ◽  
Polyamino Acid ◽  
Helicoverpa Armígera

Chymostatin is a small nonprotein polyamino acid that specifically inhibits chymotrypsinlike enzymes by covalently binding to their active site It is therefore a model for tightbinding proteinaceous protease inhibitors of chymotrypsin/elastase specificity The Lepidopteran Helicoverpa armigera was raised on an artificial diet containing 003 chymostatin After 14 days the experimental animals were approximately twentyfold smaller than the controls although there was no increase in mortality The RNA expression levels of the main trypsin gene group were found to decrease while that of the chymotrypsin/elastase gene group increased Changes in activity of the midgut protease enzymes did not correlate to these changes in RNA expression

scholarly journals Comparative TMT Proteomic Analysis Unveils Unique Insights into Helicoverpa armigera (Hübner) Resistance in Cajanus scarabaeoides (L.) Thouars

2021 ◽  
Vol 22 (11) ◽  
pp. 5941
Author(s):  
Abigail Ngugi-Dawit ◽  
Isaac Njaci ◽  
Thomas J.V. Higgins ◽  
Brett Williams ◽  
Sita R. Ghimire ◽  
...  
Keyword(s):  
Host Plant ◽  
Helicoverpa Armigera ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Proteomic Analysis ◽  
Artificial Diet ◽  
Growth Stages ◽  
Genetic Base ◽  
Helicoverpa Armígera ◽  
Helicoverpa Armigera Hubner

Pigeonpea [Cajanus cajan (L.) Millspaugh] is an economically important legume playing a crucial role in the semi-arid tropics. Pigeonpea is susceptible to Helicoverpa armigera (Hübner), which causes devastating yield losses. This pest is developing resistance to many commercially available insecticides. Therefore, crop wild relatives of pigeonpea, are being considered as potential sources of genes to expand the genetic base of cultivated pigeonpea to improve traits such as host plant resistance to pests and pathogens. Quantitative proteomic analysis was conducted using the tandem mass tag platform to identify differentially abundant proteins between IBS 3471 and ICPL 87 tolerant accession and susceptible variety to H. armigera, respectively. Leaf proteome were analysed at the vegetative and flowering/podding growth stages. H. armigera tolerance in IBS 3471 appeared to be related to enhanced defence responses, such as changes in secondary metabolite precursors, antioxidants, and the phenylpropanoid pathway. The development of larvae fed on an artificial diet with IBS 3471 lyophilised leaves showed similar inhibition with those fed on an artificial diet with quercetin concentrations with 32 mg/25 g of artificial diet. DAB staining (3,3’-diaminobenzidine) revealed a rapid accumulation of reactive oxygen species in IBS 3471. We conclude that IBS 3471 is an ideal candidate for improving the genetic base of cultivated pigeonpea, including traits for host plant resistance.

Tripeptides derived from reactive centre loop of potato type II protease inhibitors preferentially inhibit midgut proteases of Helicoverpa armigera

2018 ◽  
Vol 95 ◽  
pp. 17-25 ◽  
Author(s):  
Nidhi S. Saikhedkar ◽  
Rakesh S. Joshi ◽  
Ashiwini S. Bhoite ◽  
Radhika Mohandasan ◽  
Amit Kumar Yadav ◽  
...  
Keyword(s):  
Protease Inhibitors ◽  
Helicoverpa Armigera ◽  
Type Ii ◽  
Reactive Centre Loop ◽  
Helicoverpa Armígera

scholarly journals Rearing the Cotton Bollworm,Helicoverpa armigera, on a Tapioca-Based Artificial Diet

2007 ◽  
Vol 7 (35) ◽  
pp. 1-7 ◽  
Author(s):  
Bilal Haider Abbasi ◽  
Khalique Ahmed ◽  
Feeroza Khalique ◽  
Najma Ayub ◽  
Hai Jun Liu ◽  
...  
Keyword(s):  
Helicoverpa Armigera ◽  
Artificial Diet ◽  
Cotton Bollworm ◽  
Helicoverpa Armígera

scholarly journals Comparative biology of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) in Eucalyptus spp.

2020 ◽  
Vol 9 (7) ◽  
pp. e729974627
Author(s):  
Franciele Muchalak ◽  
Elisângela de Souza Loureiro ◽  
Luis Gustavo Amorim Pessoa
Keyword(s):  
Helicoverpa Armigera ◽  
Artificial Diet ◽  
Statistical Difference ◽  
Plant Protection ◽  
Plant Protection Products ◽  
Mato Grosso ◽  
Forestry Sector ◽  
Helicoverpa Armígera ◽  
Forestry Plantations ◽  
Lepidoptera Noctuidae

The Brazilian forestry sector has great importance in the economy and the sustainability of the country. With the great expansion of monoculture, and many times the proximity of agricultural and forestry plantations favors the migration or establishment of pests. The Helicoverpa armigera is a pest extremely polyphagous that occurs mainly in agricultural crops, and that casually was found feeding on eucalyptus plantations in the region of Chapadão do Sul, State of Mato Grosso do Sul, Brazil. In this study the parameters of consumption, size, and longevity of caterpillars were measured. The experimental design was completely randomized with five treatments (clones I 144; 08; Urocam VM01; AEC 1528 and artificial diet, this being a witness) and six replicates of 10 caterpillar larvae. The clones were grown in greenhouse conditions to produce leaves free of chemical plant protection products and with good nutritional status from which they have removed the sheets and supplied to the caterpillars. There was no statistical difference between the clones tested in none of the evaluated parameters. Only the artificial diet showed statistical difference concerning the size. The caterpillars fed on leaves of the clones have not completed their development, suggesting that the effect of antibiosis interfering on the biology of H. armigera not reaching status of pest.

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